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Optimized in-solution enrichment over a million ancient human SNPs

Davidson, Roberta; Roca-Rada, Xavier; Ravinshakar, Shyamsundar; Taufik, Leonard; Haarkötter, Christian; Collen, Evelyn; Williams, Matthew P.

Background In-solution hybridization enrichment of genetic markers is a method of choice in paleogenomic studies, where the DNA of interest is generally heavily fragmented and contaminated with environmental DNA, and where the retrieval of genetic data comparable between individuals is challenging. Here, we benchmark the commercial “Twist Ancient DNA” reagent from Twist Biosciences using sequencing libraries fr...


Variability of lesions and lesion pattern in possible treponematosis cases: Ins...

Curto, Ana; Fernandes, Teresa; Relvado, Cláudia; Ferro, Sónia; Lopes, Célia

Objective: This study aims to describe various types of skeletal lesions and lesion patterns observed in five potential cases of treponematosis from the same archaeological site. Material and methods: The study examines five adult skeletons recovered from Santa Maria do Olival, Tomar, Portugal. Macroscopic observations were conducted on all remains, supplemented by relevant radiographic investigations. Results:...


Physical Impairment and Care Estimation in Medieval Estremoz (Portugal): A Bioa...

Curto, Ana; Lopes, Célia; Maurer, Anne-France; Fernandes, Teresa

This study aims to compare diet and skeletal health indicators between individuals with varying degrees of physical impairment and the general adult population from medieval Estremoz, Portugal, to assess their health status and their survival outcomes. Skeletal remains from 41 male adult individuals, including 8 with physical impairments and 33 from the general population, were analyzed. The impairment of these...





Comparing morphological (os coxae) and metric (long bone length) sex estimation...

Curto, Ana; Ferro, Sónia; Santos, Cláudia; Lourenço, Marina; Fahy, Geraldine E.; Anselmo, Daniela; Fernandes, Teresa

Objectives: The aim of this study is to evaluate the internal consistency of sex estimation using metric (long bone length) and morphological (os coxae) methodologies from different bones and across different archaeological populations from different regions. Materials and Methods: Sex was estimated using characteristics of the pelvis and compared with sex estimations using long bone length. Portuguese (659 fem...


Abstinência alimentar: uma opção ou uma regra?

Ribeiro, Ana; Maurer, Anne-France; Relvado, Claudia; Barrocas Dias, cristina; Fernandes, Teresa; Detry, Cleia; Umbelino, Claudia

A dieta das clarissas do Mosteiro de Santa Clara-a-Velha de Coimbra (séculos XIV a XVII) foi caracterizada através da realização de análises de isótopos estáveis de carbono (δ13C) e de azoto (δ15N) no colagénio ósseo de 28 esqueletos humanos. Confrontaram-se os resultados obtidos com dados históricos, carpológicos e zooarqueológicos. Os valores observados de δ13C (entre −18,3 ‰ e −16,5 ‰) e de δ15N (entre 11,6 ...


Comparing morphological (os coxae) and metric (long bone length) sex estimation...

Curto, Ana; Ferro, Sónia; Santos, Cláudia; Lourenço, Marina; Fahy, Geraldine E.; Anselmo, Daniela; Fernandes, Teresa

Objectives: The aim of this study is to evaluate the internal consistency of sex estimation using metric (long bone length) and morphological (os coxae) methodologies from different bones and across different archaeological populations from different regions. Materials and Methods: Sex was estimated using characteristics of the pelvis and compared with sex estimations using long bone length. Portuguese (659 fem...


Clubfoot and its implications for the locomotion of a medieval skeleton from Es...

Curto, Ana; Fernandes, Teresa

Objective This study describes foot bone anomalies and the degenerative changes associated with locomotion adaptations. Materials An adult male (approximately 29–46 years old) from Estremoz, Portugal (13th–15th century). Methods The skeletal anomalies observed in this skeleton were described and measurements were compared between both sides of the individual and with the mean of the adult males from the same co...


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