5 documents found, page 1 of 1
Neurodifferentiation and neuroprotection potential of mesenchymal stromal cell-...
Marques, Cláudia Raquel; Fuzeta, Miguel de Almeida; Cunha, Raquel Medina dos Santos; Pereira-Sousa, Joana; Silva, Deolinda; Campos, Jonas OliveiraParkinson’s disease (PD) is the second most common neurodegenerative disorder and is characterized by the degeneration of the dopamine (DA) neurons in the substantia nigra pars compacta, leading to a loss of DA in the basal ganglia. The presence of aggregates of alpha-synuclein (α-synuclein) is seen as the main contributor to the pathogenesis and progression of PD. Evidence suggests that the secretome of mesenc...
Scalable production of human mesenchymal stromal cell-derived extracellular ves...
Fuzeta, Miguel de Almeida; Bernardes, Nuno; Oliveira, Filipa D.; Costa, Ana Catarina; Fernandes-Platzgummer, Ana; Farinha, José PauloMesenchymal stromal cells (MSC) hold great promise for tissue engineering and cell-based therapies due to their multilineage differentiation potential and intrinsic immunomodulatory and trophic activities. Over the past years, increasing evidence has proposed extracellular vesicles (EVs) as mediators of many of the MSC-associated therapeutic features. EVs have emerged as mediators of intercellular communication...
Mesenchymal stromal cells induce regulatory T cells via epigenetic conversion o...
Azevedo, Rita I.; Minskaia, Ekaterina; Fernandes-Platzgummer, Ana; Vieira, Ana I. S.; da Silva, Cláudia Lobato; Cabral, Joaquim M.S.; Lacerda, JoãoRegulatory T cells (Treg) play a critical role in immune tolerance. The scarcity of Treg therapy clinical trials in humans has been largely due to the difficulty in obtaining sufficient Treg numbers. We performed a preclinical investigation on the potential of mesenchymal stromal cells (MSCs) to expand Treg in vitro to support future clinical trials. Human peripheral blood mononuclear cells from healthy donors ...
Mid-infrared spectroscopy: a groundbreaking tool for monitoring mammalian cells...
Rosa, Filipa O.P.; Ribeiro Da Cunha, Bernardo; Carmelo, Joana G.; Fernandes-Platzgummer, Ana; da Silva, Claudia; Calado, CecíliaMammalian cells are extensively used in cell biology studies, e.g. as a model system of human pathologies, or as a major source of very high-value biopharmaceuticals (that can be the cells itself or their products e.g. heterologous proteins). As such, it is highly pertinent to develop monitoring methods for mammalian cultivations capable of delivering detailed bioprocess information in a rapid and economic way....
Monitoring the ex-vivo expansion of human mesenchymal stem/stromal cells in xen...
Rosa, Filipa; Sales, Kevin C.; Carmelo, Joana G.; Fernandes-Platzgummer, Ana; Silva, Cláudia L. da; Lopes, Marta B.; Calado, CecíliaHuman mesenchymal stem/stromal cells (MSCs) have received considerable attention in the field of cell-based therapies due to their high differentiation potential and ability to modulate immune responses. However, since these cells can only be isolated in very low quantities, successful realization of these therapies requires MSCs ex-vivo expansion to achieve relevant cell doses. The metabolic activity is one of...