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Study of the association between genotypic potential and linoleic acid toleranc...

Fontes, Ana Luiza; Pimentel, Lígia Leão; Calzada, Javier; Salsinha, Ana Sofia; Rodríguez, Juan Miguel; Gomes, Ana Maria; Arqués, Juan L.

Probiotic bacteria have been shown to produce the bioactive conjugated linoleic acid (CLA) from linoleic acid (LA), this being associated with a detoxifying mechanism. Primers employed in genotypic selection are generally species-specific. Therefore, this work aimed to identify CLA producers by previously screening genes encoding enzymes involved in LA isomerization with primers designed based on conserved moti...


Perspectives on the development of CLA/CLNA-enriched milk through in situ micro...

Fontes, Ana Luiza; Pimentel, Lígia; Calzada, Javier; Salsinha, Ana Sofia; Rodríguez, Juan Miguel; Arqués, Juan; Domingues, Maria do Rosário

Over the past decade several food-derived lipids with potential bioactive properties to be used in the development of innovative functional foods have been identified. These include conjugated linoleic (CLA) and conjugated linolenic (CLNA) acids with well described anti- inflammatory, anti-obesity and anticarcinogenic properties. Limited availability in their natural sources (e.g. ruminants’ milk and meat or ve...


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