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Speeding up the diagnosis of multidrug-resistant tuberculosis in a high-burden ...
Brandao,Angela Pires; Pinhata,Juliana Maira Watanabe; Oliveira,Rosangela Siqueira; Galesi,Vera Maria Neder; Caiaffa-Filho,Helio HehlABSTRACT Objective: To evaluate the rapid diagnosis of multidrug-resistant tuberculosis, by using a commercial line probe assay for rifampicin and isoniazid detection (LPA-plus), in the routine workflow of a tuberculosis reference laboratory. Methods: The LPA-plus was prospectively evaluated on 341 isolates concurrently submitted to the automated liquid drug susceptibility testing system. Results: Among 303 phe...
Pulmonary nontuberculous mycobacterial infections: presumptive diagnosis based ...
Marques,Lilian Regina Macelloni; Ferrazoli,Lucilaine; Chimara,ÉricaABSTRACT Objective: Pulmonary nontuberculous mycobacterial infections are caused by nontuberculous mycobacteria (NTM), the microbiological diagnosis of which involves the isolation and identification of the same species in at least two sputum samples, one BAL fluid sample, or one lung biopsy sample. The objective of the present study was to determine the frequency at which the various NTM species are identified...
First insights into the genetic diversity of Mycobacterium tuberculosis strains...
Silva,Aída Cristina do Nascimento; Ferrazoli,Lucilaine; Simonsen,Vera; Reis,Joice Neves; Pereira,Susan Martins; Carmo,Theomira Mauadie de AzevedoThis study constitutes a first attempt to describe the genetic population structure of Mycobacterium tuberculosis circulating in Salvador, Bahia State, Brazil. A total of 56 confirmed cases of pulmonary tuberculosis, identified between March and June 2008, were analyzed using restriction fragment length polymorphism (IS6110-RFLP). The study population was characterized by a predominance of males (71.43%) over 3...
Identificação laboratorial de micobactérias em amostras respiratórias de pacien...
Zamarioli,Liliana Aparecida; Coelho,Andréa Gobetti Vieira; Pereira,Clemira Martins; Ferrazoli,Lucilaine; Bammann,Ricardo HelbertForam analisados retrospectivamente os registros (2000 a 2004) do Laboratório de Microbiologia do Instituto Adolfo Lutz de Santos, SP referentes a pacientes infectados pelo virus da imunodeficiência humana com suspeita de tuberculose pulmonar. Foram encaminhadas 1.321 amostras com finalidade de diagnóstico, correspondendo a 880 casos suspeitos de tuberculose em 693 pacientes. Cento e trinta e quatro baciloscopi...
Micobactérias não-tuberculosas: diversidade das espécies no estado de São Paulo
Ueki,Suely Yoko Mizuka; Martins,Maria Conceição; Telles,Maria Alice da Silva; Virgilio,Melissa Curcio; Giampaglia,Carmen Maria Saraiva; Chimara,EricaO gênero Mycobacterium é constituído por espécies do complexo M. tuberculosis e outras denominadas micobactérias não-tuberculosas (MNT). Até o momento, mais de cem MNT foram descritas. Os objetivos deste estudo foram avaliar a diversidade das espécies de MNT identificadas no estado de São Paulo, no período de 1991 a 1997, que antecedeu a expansão da terapia anti-retroviral, e determinar a freqüência dos casos q...