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Unlocking the Gates

Patil, Mrunal; Munteanu, Tatiana; Brasseur, Gaël; Ferreira, Carolina; Costa, Sofia Santos; Couto, Isabel; Athar, Mohd; Asunis, Elisa

Funding Information: All authors contributed equally to this work. This project was partially funded by the A*Midex Foundation Interdisciplinarity Grant \u201CElisa.\u201D M.P. was supported by the CNRS\u2010Innovation \u201CPhenux\u201D Grant. M.A. and A.V.V. gratefully acknowledge the \u201COne Health Basic and Translational Research Actions addressing Unmet Needs on Emerging Infectious Diseases (INF\u2010ACT...


Genetic diversity and antimicrobial resistance profiles of Staphylococcus pseud...

Morais, Catarina; Costa, Sofia Santos; Leal, Marta; Ramos, Bárbara; Andrade, Mariana; Ferreira, Carolina; Abrantes, Patrícia; Pomba, Constança

Funding Information: This study was supported by Project BIOSAFE funded by FEDER through the Programa Operacional Factores de Competitividade–COMPETE, by the Fundação para a Ciência e a Tecnologia (FCT, Portugal) - Grant LISBOA-01-0145-FEDER-030713, PTDC/CAL-EST/30713/2017 and by FCT through grants UI/BD/151061/2021 (CM) and 2021.05063.BD (CF) and funds to GHTM (UID/04413/2020) and the CIISA Project (UID/CVT/00...


Assessment of chlorhexidine activity against Staphylococcus pseudintermedius fr...

Morais, Catarina; Ferreira, Carolina; Zacharias, Paula; Abrantes, Patrícia; Pomba, Constança; Costa, Sofia Santos; Couto, Isabel

Background Staphylococcus aureus and Staphylococcus pseudintermedius are responsible for skin and soft-tissue infections (SSTIs) in companion animals and are increasingly associated with antimicrobial resistance (AMR). Chlorhexidine (CHX) is one of the most used and recommended antisseptic in the treatment of SSTIs in these animals. This study analyzed the effectiveness, at different conditions, of CHX against ...


Chlorhexidine and benzalkonium chloride activity against Staphylococcus aureus ...

Ferreira, Carolina; Morais, Catarina; Zacharias, Paula; Abrantes, Patrícia; Pomba, Constança; Costa, Sofia Santos; Couto, Isabel


Occurence and variability of norA and other main multidrug efflux pump determin...

Ferreira, Carolina; Abrantes, Patrícia; Costa, Sofia Santos; Viveiros, Miguel; Couto, Isabel

Background Multidrug efflux pumps (MDR EPs) play a major role in the emergence of antimicrobial resistance (AMR). NorA is one of the main native MDR EPs of Staphylococcus aureus, contributing to reduced susceptibility towards fluoroquinolones and biocides[1,2]. In S. aureus, its gene occurs in several allelic variants[3], but less is known about the occurrence of norA and other MDR EPs across the Staphylococcus...


Occurrence and Variability of the Efflux Pump Gene norA across the Staphylococc...

Ferreira, Carolina; Abrantes, Patrícia; Costa, Sofia Santos; Viveiros, Miguel; Couto, Isabel

Funding Information: This work was supported by Project BIOSAFE funded by FEDER through the Programa Operacional Factores de Competitividade—COMPETE and by Fundação para a Ciência e a Tecnologia (FCT, Portugal)—Grant LISBOA-01-0145-FEDER-030713, PTDC/CAL-EST/30713/2017 and by FCT through grant 2021.05063.BD (C.F) and funds to GHTM (UID/04413/2020). Publisher Copyright: © 2022 by the authors.; NorA is one of the...


Staphylococcus aureus Causing Skin and Soft Tissue Infections in Companion Anim...

Costa, Sofia Santos; Ribeiro, Rute; Serrano, Maria; Oliveira, Ketlyn; Ferreira, Carolina; Leal, Marta; Pomba, Constança; Couto, Isabel

Staphylococcus aureus is a relevant agent of skin and soft tissue infections (SSTIs) in animals. Fifty-five S. aureus comprising all SSTI-related isolates in companion animals, collected between 1999 and 2018 (Lab 1) or 2017 and 2018 (Lab 2), were characterized regarding susceptibility to antibiotics and heavy metals and carriage of antimicrobial resistance determinants. Clonal lineages were established by PFGE...


Clonal lineages, antimicrobial resistance, and pvl carriage of staphylococcus a...

Ferreira, Carolina; Costa, Sofia Santos; Serrano, Maria; Oliveira, Ketlyn; Trigueiro, Graça; Pomba, Constança; Couto, Isabel

Staphylococcus aureus (S. aureus) is a leading cause of skin and soft-tissue infections (SSTIs) in the community. In this study, we characterized a collection of 34 S. aureus from SSTIs in ambulatory patients in Portugal and analyzed the presence of Panton–Valentine leucocidin (PVL)-encoding genes and antibiotic-resistance profile, which was correlated with genetic determinants, plasmid carriage, and clonal lin...


Proposal of Epidemiological Cutoff Values for Apramycin 15 μg and Florfenicol 3...

Costa, Sofia Santos; Ferreira, Carolina; Ribeiro, Rute; Feßler, Andrea T; Schink, Anne-Kathrin; Kadlec, Kristina; Kaspar, Heike; Amaro, Ana

Funding Information This study was supported by Project BIOSAFE funded by FEDER through the Programa Operacional Factores de Competitividade–COMPETE and by Fundação para a Ciência e a Tecnologia (FCT, Portugal)—Grant LISBOA-01-0145-FEDER-030713, PTDC/CAL-EST/30713/2017 and by FCT through funds to GHTM (UID/04413/2020), CIISA Project (UID/CVT/00276/2020), and Project PTDC/CVT-CVT/28469/2017. The contributions of...


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