4 documents found, page 1 of 1
The Aspergillus fumigatus Mismatch Repair MSH2 Homolog Is Important for Virulen...
Dos Reis, Thaila Fernanda; Silva, Lilian Pereira; de Castro, Patrícia Alves; do Carmo, Rafaela Andrade; Marini, Marjorie MendesThe genetic stability of every living organism depends on accurate DNA replication and repair systems. Here, we investigated the Aspergillus fumigatusMSH2 mismatch repair (MMR) gene MshA and how it impacts virulence and the evolution of azole resistance. We examined mshA gene variation in 62 environmental and clinical A. fumigatus strains. We have observed 12 strains with variants (18.2%), and 8 strains among t...
Synthetic and minimalist vectors for Agrobacterium tumefaciens-mediated transfo...
Nora, Luísa Czamanski; Gonçales, Relber Aguiar; Martins-Santana, Leonardo; Ferreira, Beatriz Henriques; Rodrigues, Fernando José dos SantosWe present a collection of minimalist binary vectors for transformation through ATMT applicable to several fungi species. pLUO plasmid binary vectors consist of a reporter module containing fluorescent proteins, mCherry or eGFP, flanked by a multiple cloning site and a transcription terminator site. They also present a synthetic gene allowing resistance to Hygromicin B flanked by alternate promoters, one for ye...
The influence of genetic stability on Aspergillus fumigatus virulence and azole...
Reis, Thaila Fernanda dos; Silva, Lilian Pereira; Castro, Patrícia Alves; Lima, Pollyne Borborema Almeida de; Carmo, Rafaela Andrade doGenetic stability is extremely important for the survival of every living organism, and a very complex set of genes has evolved to cope with DNA repair upon DNA damage. Here, we investigated the Aspergillus fumigatus AtmA (Ataxia-telangiectasia mutated, ATM) and AtrA kinases, and how they impact virulence and the evolution of azole resistance. We demonstrated that A. fumigatus atmA and atrA null mutants are hap...
Sporothrix brasiliensis – from the genome to the phenotype
Ferreira, Beatriz HenriquesSporotrichosis is the most prevalent subcutaneous mycosis worldwide and increasing number of cases is being reported in the last decade. It is caused by thermodimorphic fungi of the Sporothrix schenckii complex and affects immunosuppressed and immunocompetent individuals. S. brasiliensis is the main causing agent of this mycosis in Brazil, where sporotrichosis is hyperendemic. This organism presents an increase...