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In Vitro CRISPR/Cas9 Transfection and Gene-Editing Mediated by Multivalent Cati...
Sousa, Diana Andrade; Gaspar, Ricardo; Ferreira, Celso J. O.; Baltazar, Fátima; Rodrigues, L. R.; Silva, Bruno F. B.Clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated nuclease 9 (Cas9) gene-editing offers exciting new therapeutic possibilities for disease treatment with a genetic etiology such as cancer, cardiovascular, neuronal, and immune disorders. However, its clinical translation is being hampered by the lack of safe, versatile, and effective nonviral delivery systems. Herein we rep...
pH-sensitive nanoliposomes for passive and CXCR-4-mediated marine yessotoxin de...
Vieira, Ana M. G.; Silvestre, Oscar F.; Silva, Bruno F. B.; Ferreira, Celso J. O.; Lopes, Ivo Edgar Araújo; Gomes, Andreia C; Espiña, BegoñaBackground: Yessotoxin (YTX), a marine-derived drug, was encapsulated in PEGylated pH-sensitive nanoliposomes, covalently functionalized (strategy I) with SDF-1α and by nonspecific adsorption (strategy II), to actively target chemokine receptor CXCR-4. Methods: Cytotoxicity to normal human epithelial cells (HK-2) and prostate (PC-3) and breast (MCF-7) adenocarcinoma models, with different expression levels of C...
Cellular delivery of CRISPR/Cas9 plasmid using multivalent cationic liposomes
Sousa, Diana Andrade; Gaspar, Ricardo; Ferreira, Celso J. O.; Baltazar, Fátima; Silva, Bruno F. B.; Rodrigues, L. R.[Excerpt] Background: Gene therapy techniques have been aimed at mitigating disease features of recessive and dominant disorders, as well as several cancers, cardiovascular, neuronal and immune diseases [1]. In recent years, the clustered regularly interspaced short palindromic repeats (CRISPR) and Cas9 protein system has been gaining recognition as a revolutionary tool for gene therapy. However, the implementa...
Microfluidics for controlled self-assembly of cubosome nanoparticles of tunable...
Ferreira, Celso J. O.; Barros, Margarida; Botelho, Cláudia; Real Oliveira, M. Elisabete C.D.; Silva, Bruno F. B.