21 documents found, page 1 of 3
Liposomal formulations for efficient delivery of a novel, highly potent pyrimid...
Teixeira, Sofia; Ferreira, Débora Carina Gonçalves Abreu; Rodrigues, Ana Rita Oliveira; Rodrigues, L. R.; Castanheira, Elisabete M. S.Background/Objectives: Cancer is one of the deadliest diseases worldwide. Despite the existing treatments, the adverse side effects and the increasing drug resistance to the current therapies lead to a reduced quality of life for patients and poor prognosis. The pyrimido[5,4-d]pyrimidine compound (PP) was identified as a promising new anticancer drug due to its potent activity against colorectal and triple-nega...
Albumin/Hyaluronic acid gel nanoparticles loaded with a pyrimidine-based drug f...
Teixeira, Sofia; Ferreira, Débora Carina Gonçalves Abreu; Rodrigues, L. R.; Carvalho, M. Alice; Castanheira, Elisabete M. S.A pyrimidine-based compound (PP) was recently found to be a promising anticancer agent for colorectal and breast cancers. However, this compound exhibited low selectivity and poor water solubility. To address these challenges, albumin gel nanoparticles were used, where the gel matrix is formed by cross-linking of BSA molecules, allowing for a high concentration of this hydrophobic drug to be carried with no cyt...
Nanoformulações de um novo composto muito promissor para terapia do cancro da m...
Teixeira, Marta Sofia Vilela Barreira; Ferreira, Débora Carina Gonçalves Abreu; Rodrigues, L. R.; Castanheira, Elisabete M. S.; Carvalho, M. AliceNo presente estudo pretendeu-se desenvolver, caracterizar e avaliar o efeito antitumoral de nanoformulações carregadas com o composto Hit. As formulações carregadas com o composto hit foram caracterizadas por DLS (tamanho hidrodinâmico, índice de polidispersividade e potencial zeta), e a cinética de libertação do composto. Adicionalmente, os efeitos antitumorais das nanoformulações foram determinados na linha c...
Lactoferrin-loaded magnetic liposomes as potential photothermal agents for brea...
Andrade, Raquel G. D.; Santos-Pereira, Cátia; Ferreira, Débora Carina Gonçalves Abreu; Castanheira, Elisabete M. S.; Rodrigues, L. R.Nanoparticle-based drug delivery systems can afford an alternative treatment for breast cancer, overcoming conventional therapy’s limitations. In particular, superparamagnetic iron oxide nanoparticles entrapped in liposomes have gained significant interest in the biomedical field due to their exceptional properties, like enabling their accumulation at the intended target site when exposed to an external magneti...
Citrate-coated manganese and calcium ferrite nanoparticles as potential magneti...
Andrade, Raquel G. D.; Ferreira, Débora Carina Gonçalves Abreu; Veloso, Sérgio Rafael Silva; Santos-Pereira, Cátia; Castanheira, Elisabete M. S.In this work, manganese/calcium ferrite NPs coated with citrate, were synthesized by the sol-gel method, followed by calcination, and then characterized regarding their crystalline structure (by X-ray diffraction, XRD), size and shape (by Transmission Electron Microscopy, TEM), hydrodynamic size and zeta potential (by Dynamic Light Scattering, DLS), and heating efficiency (measuring the Specific Absorption Rate...
Glucose metabolism reprogramming in bladder cancer: hexokinase 2 (HK2) as progn...
Afonso, Julieta; Gonçalves, Céline; Costa, Marta; Ferreira, Débora Carina Gonçalves Abreu; Santos, Lúcio; Longatto-Filho, Adhemar; Baltazar, FátimaProliferating cancer cells are able to reprogram their energy metabolism, favouring glycolysis even in the presence of oxygen and fully functioning mitochondria. Research is needed to validate the glycolysis-related proteins as prognostic/predictive biomarkers in urothelial bladder carcinoma (UBC), a malignancy tagged by high recurrence rates and poor response to chemotherapy. Here, we assessed GLUT1, HK2, PFKL...
Design of new hydrolyzed collagen-modified magnetic nanoparticles to capture pa...
Sande, Maria Georgina; Roque, Lúcia; Braga, Adelaide Correia; Marques, Márcia; Ferreira, Débora Carina Gonçalves Abreu; Saragliadis, AthanasiosEnrichment and diagnosis tools for pathogens currently available are time consuming, thus the development of fast and highly sensitive alternatives is desirable. In this study, a novel approach was described that enables selective capture of bacteria expressing hydrolyzed collagen-binding adhesins with hydrolyzed collagen-coated magnetic nanoparticles (MNPs). This platform could be useful to shorten the time ne...
Exosomes modified with anti-MEK1 siRNA lead to an effective silencing of triple...
Ferreira, Débora Carina Gonçalves Abreu; Pereira, Cátia Sofia Santos; Costa, Marta; Afonso, Julieta Alexandra Pereira; Yang, Sujuan; Hensel, JanineTriple negative breast cancer (TNBC) is a highly heterogenous disease not sensitive to endocrine or HER2 therapy and standardized treatment regimens are still missing. Therefore, development of novel TNBC treatment approaches is of utmost relevance. Herein, the potential of MAPK/ERK downregulation by RNAi-based therapeutics in a panel of mesenchymal stem-like TNBC cell lines was uncovered. Our data revealed tha...
In vitro selection of DNA aptamers against human osteosarcoma
Tsogtbaatar, Khaliunsarnai; Sousa, Diana Andrade; Ferreira, Débora Carina Gonçalves Abreu; Tevlek, Atakan; Aydin, Halil Murat; Çelik, EdaBackground. Osteosarcoma is a highly malignant bone tumor, most frequently occurring in the rapid bone growth phase. Effective treatment of this disease is hindered by the lack of specific probes for early diagnosis and the fast cancer widespread. Methods. To find such probes, the cell-Systematic Evolution of Ligands by EXponential enrichment (cell-SELEX) methodology was implemented against the human osteosarco...
Lactoferrin perturbs intracellular trafficking, disrupts cholesterol-rich lipid...
Pereira, Cátia Sofia Santos; Guedes, Joana Catarina Pereira; Ferreira, Débora Carina Gonçalves Abreu; Rodrigues, L. R.; Côrte-Real, ManuelaThe milk-derived bovine lactoferrin (bLf) is an iron-binding glycoprotein with remarkable selective anticancer activity towards highly metastatic cancer cells displaying the proton pump V-ATPase at the plasma membrane. As studies aiming to dissect the bLf mechanisms of action are critical to improve its efficacy and boost its targeted clinical use, herein we sought to further uncover the molecular basis of bLf ...