89 documents found, page 1 of 9
Cellular reprogramming as a tool to model human aging in a dish
Pitrez, Patrícia R.; Monteiro, Luís M.; Borgogno, Oliver; Nissan, Xavier; Mertens, Jerome; Ferreira, LinoThe design of human model systems is highly relevant to unveil the underlying mechanisms of aging and to provide insights on potential interventions to extend human health and life span. In this perspective, we explore the potential of 2D or 3D culture models comprising human induced pluripotent stem cells and transdifferentiated cells obtained from aged or age-related disorder-affected donors to enhance our un...
Near-Infrared Light Activated Formulation for the Spatially Controlled Release ...
Simões, Susana; Lino, Miguel; Barrera, Angela; Rebelo, Catarina; Tomatis, Francesca; Vilaça, Andreia; Breunig, Christopher; Neuner, Andrea; Peça, JoãoThe CRISPR/Cas9 system has emerged as a promising platform for gene editing; however, the lack of an efficient and safe delivery system to introduce it into cells continues to hinder clinical translation. Here, we report a rationally designed gene-editing nanoparticle (NP) formulation for brain applications: an sgRNA:Cas9 ribonucleoprotein complex is immobilized on the NP surface by oligonucleotides that are co...
Unveiling the antitumor mechanism of 7α-acetoxy-6β-hydroxyroyleanone from Plect...
Magalhães, Mariana; Domínguez-Martín, Eva María; Jorge, Joana; Gonçalves, Ana Cristina; Massenzio, Francesca; Spigarelli, RenatoGlioblastoma (GB) is the most aggressive and prevalent glioma within the central nervous system. Despite considerable efforts, GB continues to exhibit a dismal 5-year survival rate (∼6%). This is largely attributed to unfavorable prognosis and lack of viable treatment options. Therefore, novel therapies centered around plant-derived compounds emerge as a compelling avenue to enhance patient survival and well-be...
Blood-brain barrier dysfunction in aging is mediated by brain endothelial senes...
Novo, João P.; Gee, Lucy; Caetano, Carolina A; Tomé, Inês; Vilaça, Andreia; von Zglinicki, Thomas; Moreira, Irina S; Jurk, Diana; Rosa, SusanaBBB dysfunction during aging is characterized by an increase in its permeability and phenotypic alterations of brain endothelial cells (BECs) including dysregulation of tight junction's expression. Here we have investigated the role of BEC senescence in the dysfunction of the BBB. Our results suggest that the transition from young to aged BBB is mediated, at least in part by BEC senescence.; SR acknowledges the...
Extracellular vesicle transfer of lncRNA H19 splice variants to cardiac cells
Vilaça, Andreia; Jesus, Carlos; Lino, Miguel; Hayman, Danika; Emanueli, Costanza; Terracciano, Cesare M.; Fernandes, Hugo; de Windt, Leon J.CC BY-NC-ND 4.0; The authors would like to acknowledge Crioestaminal (www.crioestaminal.pt) for providing samples and other helpful information and Dr. Yingqun Huang for providing the lncRNA H19 plasmid. The authors would further like to acknowledge the funding by the FCT PhD Studentships (SFRH/BD/119187/2016 & SFRH/BD/ 144092/2019), Programa Operacional Competividade e Internacionalização (POCI) na sua compone...
Shape-versatile fixed cellular materials for multiple target immunomodulation
Sousa, Ana Rita; Cunha, Ana F.; Santos-Coquillat, Ana; Estrada, Beatriz Hernaez; Spiller, Kara L.; Barão, Marta; Rodrigues, Artur Filipe; Simões, SusanaTherapeutic cells are usually administered as living agents, despite the risks of undesired cell migration and acquisition of unpredictable phenotypes. Additionally, most cell-based therapies rely on the administration of single cells, often associated with rapid in vivo clearance. Three-dimensional cellular materials may be useful to prolong the effect of cellular therapies and offer the possibility of creatin...
Roadmap on nanomedicine for the central nervous system
Ciofani, Gianni; Campisi, Marco; Mattu, Clara; Kamm, Roger D.; Chiono, Valeria; Raynold, Aji Alex Moothedathu; Freitas, João S.; Redolfi Riva, EugenioIn recent years, a great deal of effort has been undertaken with regards to treatment of pathologies at the level of the central nervous system (CNS). Here, the presence of the blood-brain barrier acts as an obstacle to the delivery of potentially effective drugs and makes accessibility to, and treatment of, the CNS one of the most significant challenges in medicine. In this Roadmap article, we present the stat...
A Polymeric Nanoparticle Formulation for Targeted mRNA Delivery to Fibroblasts
Rodrigues, Artur Filipe; Rebelo, Catarina; Simões, Susana; Paulo, Cristiana; Pinho, Sónia A.; Francisco, Vitor; Ferreira, LinoMessenger RNA (mRNA)-based therapies offer enhanced control over the production of therapeutic proteins for many diseases. Their clinical implementation warrants formulations capable of delivering them safely and effectively to target sites. Owing to their chemical versatility, polymeric nanoparticles can be designed by combinatorial synthesis of different ionizable, cationic, and aromatic moieties to modulate ...
Protein-Nanoparticle Interactions Govern the Interfacial Behavior of Polymeric ...
Traldi, Federico; Liu, Pengfei; Albino, Inês; Ferreira, Lino; Zarbakhsh, Ali; Resmini, MarinaBiomedical applications of nanoparticles require a fundamental understanding of their interactions and behavior with biological interfaces. Protein corona formation can alter the morphology and properties of nanomaterials, and knowledge of the interfacial behavior of the complexes, using in situ analytical techniques, will impact the development of nanocarriers to maximize uptake and permeability at cellular in...
Reconsidering the role of blood-brain barrier in Alzheimer's disease: From deli...
Sousa, João André; Bernardes, Catarina; Bernardo-Castro, Sara; Lino, Miguel; Albino, Inês; Ferreira, Lino; Brás, José; Guerreiro, RitaThe existence of a selective blood-brain barrier (BBB) and neurovascular coupling are two unique central nervous system vasculature features that result in an intimate relationship between neurons, glia, and blood vessels. This leads to a significant pathophysiological overlap between neurodegenerative and cerebrovascular diseases. Alzheimer's disease (AD) is the most prevalent neurodegenerative disease whose p...