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High-solids processing of Palmaria palmata for feed applications: effects of al...

Ramos-Oliveira, Catarina; Ferreira, Marta Alexandra Lages; Belo, Isabel; Oliva-Teles, Aires; Peres, Helena

Macroalgae are increasingly recognized as a valuable source of nutrients and bioactive compounds for animal nutrition, including for aquatic species. However, the complex structure of the macroalgal cell wall limits the accessibility of intracellular components, restricting their use in feeds. To overcome this limitation, macroalgal hydrolysis using various technological treatments has been tested, often employ...


Effects of solid-state fermentation of Gelidium corneum by-product on immune st...

Ferreira, Marta Alexandra Lages; Ramos-Oliveira, Catarina; Magalhães, Rui; Martins, Nicole; Serra, Cláudia R.; Salgado, José Manuel Seara; Belo, Isabel

It was previously observed that dietary inclusion of 10 % Gelidium corneum by-product and sunflower cake mixture (1:1 ratio; Gmix), replacing corn gluten meal, reduced feed utilization efficiency in European sea bass juveniles, while the same mixture fermented with Aspergillus ibericus restored feed efficiency as well as nitrogen and energy utilization, achieving levels comparable to the control diet. The prese...


Effect of solid-fermented brewers spent grain on growth, metabolism, and oxidat...

Estevão-Rodrigues, Tássia; Fernandes, Helena; Moutinho, Sara; Ferreira, Marta Alexandra Lages; Castro, Carolina; Belo, Isabel; Salgado, José Manuel

Replacing traditional agricultural ingredients with biotechnologically improved agro-industry by-products in fish diets promotes sustainable aquaculture, reduces production costs and carbon footprint, and promotes a circular economy. Brewers spent grain (BSG) is one such by-product. Solid-state fermentation (SSF) of BSG with Aspergillus ibericus enhances its nutritional value and digestibility for European seab...


Effectiveness of high-solid loading treatments to enhance nutrient and antioxid...

Ramos-Oliveira, Catarina; Ferreira, Marta Alexandra Lages; Belo, Isabel; Oliva-Teles, Aires; Peres, Helena

Macroalgae have low nutrient bioavailability, often requiring pretreatmentsphysical, chemical, or biologicaltypically using low-solid loading hydrolysis, which produces separate liquid and solid phases. In contrast, high-solid loading hydrolysis offers a single-phase alternative, though it remains underexplored for macroalgae. This study evaluated the effectiveness of high-solid loading hydrolysis for breaking ...


Aspergillus ibericus biomass production by submerged fermentation of Codium tom...

Ferreira, Marta Alexandra Lages; Salgado, José Manuel Seara; Peres, Helena; Belo, Isabel

Seaweeds are potential feedstocks for biorefinery approaches, owing to their carbohydrate content, high biomass production and lack of lignin content, which makes saccharification more feasible. The seaweed sugar-rich hydrolysates can be used to produce alternative protein sources, such as mycoprotein, to meet the increasing global protein needs in a sustainable way. Therefore, this study aimed to produce bioma...


Novel biorefinery of seaweeds using sequential bioprocessing to obtain value-ad...

Ferreira, Marta Alexandra Lages

Para sustentar a expansão da indústria da aquacultura, a procura por ingredientes sustentáveis e económicos para incluir nas rações está a aumentar. As macroalgas são um potencial recurso para incorporar nas rações, devido à sua riqueza em nutrientes e à ausência de competição com as plantas terrestres pelos usos de terra e água doce. No entanto, o seu teor em polissacarídeos prejudica a sua inclusão nas rações...


Valorization of Gelidium corneum by-product through solid-state fermentation

Ferreira, Marta Alexandra Lages; Salgado, José Manuel Seara; Peres, Helena; Belo, Isabel

Red seaweed Gelidium is used for agar extraction, generating large quantities of by-products (red seaweed by-product RSB). Its carbohydrate and protein-rich composition makes it a suitable substrate to produce value-added compounds through solid-state fermentation (SSF). In this study, RSB was used in SSF with Aspergillus ibericus and Aspergillus niger for carbohydrases production and protein enrichment. RSB wa...


Effect of solid-state fermentation of brewer's spent grain on digestibility and...

Estevão-Rodrigues, Tássia; Fernandes, Helena; Moutinho, Sara; Filipe, Diogo; Fontinha, Filipa; Magalhães, Rui; Couto, Ana

This study assessed the effects of dietary inclusion of solid-state fermented (SSF) brewers spent grain (BSG) with Aspergillus ibericus on nutrient and energy digestibility, digestive enzyme activity, and intestinal histomorphology of juvenile European seabass (Dicentrarchus labrax). Five diets (18% crude lipids and 45% crude protein) were formulated, including a control diet (without BSG), two diets with 10% a...


Enrichment of a plant feedstuff mixture's nutritional value through solid-state...

Filipe, Diogo; Vieira, Lúcia; Ferreira, Marta Alexandra Lages; Oliva-Teles, Aires; Salgado, José; Belo, Isabel; Peres, Helena

Plant feedstuffs are the main ingredients of animal feed. Owing to food–feed competition, increasing the utilization efficiency of these feedstuffs is important for animal nutrition. This can be achieved via solid-state fermentation (SSF). SSF of a plant feedstuff mixture (PFM) (25% rapeseed meal, soybean meal, rice bran, and sunflower meal) by three fungi (Aspergillus ibericus MUM 03.29, Aspergillus niger CECT...


Valorization of Gelidium corneum by-product through solid-state fermentation

Ferreira, Marta Alexandra Lages; Salgado, José Manuel; Peres, Helena; Belo, Isabel

Industrial agar extraction of red seaweed Gelidium produces a significant amount of by- products (red seaweed by-product - RSB), which are often discarded. Nevertheless, their rich composition in carbohydrates and proteins makes them appropriate for value-added compound production through solid-state fermentation (SSF). In this study, RSB was utilized as substrate for SSF with Aspergillus ibericus MUM 03.49 and...


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