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Origin and diversification of Xanthomonas citri subsp. citri pathotypes reveale...

Patané, José Salvatore Leister; Martins Junior, Joaquim; Rangel, Luiz Thiberio; Belasque Junior, José; Digiampietri, Luciano Antonio

Xanthomonas citri subsp. citri pathotypes cause bacterial citrus canker, being responsible for severe agricultural losses worldwide. The A pathotype has a broad host spectrum, while A* and Aw are more restricted both in hosts and in geography. Two previous phylogenomic studies led to contrasting well-supported clades for sequenced genomes of these pathotypes. No extensive biogeographical or divergence dating an...

Date: 2020   |   Origin: Oasisbr

Serratia liquefaciens FG3 isolated from a metallophyte plant sheds light on the...

Caneschi, Washington Luiz; Sanchez, Angelica Bianchini; Felestrino, Érica Barbosa; Lemes, Camila Gracyelle de Carvalho; Cordeiro, Isabella Ferreira

Serratia liquefaciens strain FG3 (SlFG3), isolated from the flower of Stachytarpheta glabra in the Brazilian ferruginous fields, has distinctive genomic, adaptive, and biotechnological potential. Herein, using a combination of genomics and molecular approaches, we unlocked the evolution of the adaptive traits acquired by S1FG3, which exhibits the second largest chromosome containing the largest conjugative plas...

Date: 2020   |   Origin: Oasisbr

Proteomics-based identification of differentially abundant proteins reveals ada...

Moreira, Leandro Marcio; Silva, Marcia Regina Soares da; Facincani, Agda Paula; Ferreira, Cristiano Barbalho; Ferreira, Rafael Marini

Background: Xanthomonas citri subsp. citri (Xac) is the causal agent of citrus canker. A proteomic analysis under in planta infectious and non-infectious conditions was conducted in order to increase our knowledge about the adaptive process of Xac during infection. Results: For that, a 2D–based proteomic analysis of Xac at 1, 3 and 5 days after inoculation, in comparison to Xac growth in NB media was carried ou...

Date: 2019   |   Origin: Oasisbr

A TALE of transposition : Tn3-like transposons play a major role in the spread ...

Ferreira, Rafael Marini; Oliveira, Amanda Carolina Paulino de; Moreira, Leandro Marcio; Belasque Junior, José; Gourbeyre, Edith; Siguier, Patricia

Members of the genus Xanthomonas are among the most important phytopathogens. A key feature of Xanthomonas pathogenesis is the translocation of type III secretion system (T3SS) effector proteins (T3SEs) into the plant target cells via a T3SS. Several T3SEs and a murein lytic transglycosylase gene (mlt, required for citrus canker symptoms) are found associated with three transposition-related genes in Xanthomona...

Date: 2017   |   Origin: Oasisbr

Unravelling potential virulence factor candidates in Xanthomonas citri. subsp. ...

Ferreira, Rafael Marini; Moreira, Leandro Marcio; Ferro, Jesus Aparecido; Silva, Marcia Regina Soares da; Laia, Marcelo Luiz de

Citrus canker is a major disease affecting citrus production in Brazil. It's mainly caused by Xanthomonas citri subsp. citri strain 306 pathotype A (Xac). We analysed the differential expression of proteins secreted by wild type Xac and an asymptomatic mutant for hrpB4 (1hrpB4) grown in Nutrient Broth (NB) and a medium mimicking growth conditions in the plant (XAM1). This allowed the identification of 55 secret...

Date: 2016   |   Origin: Oasisbr

Proteome of the phytopathogen Xanthomonas citri subsp. citri : a global express...

Soares, Márcia Regina; Fancicani, Agda Paula; Ferreira, Rafael Marini; Moreira, Leandro Marcio; Oliveira, Julio Cezar Franco de; Ferro, Jesus Aparecido

Background: Citrus canker is a disease caused by Xantomonas citri subsp.citri (Xac), and has emerged as one of the major threats to the worldwide citrus crop because it affects all commercial citrus varieties, decreases the production and quality of the fruits and can spread rapidly in citrus growing areas. In this work, the first proteome of Xac was analyzed using two methodologies, two-dimensional liquid chro...

Date: 2015   |   Origin: Oasisbr

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