19 documents found, page 1 of 2
Screening and in silico characterization of prophages in Helicobacter pylori cl...
Ferreira, Rute Vanessa Novais; Pinto, Graça; Presa, Eva; Oleastro, Mónica; Silva, Catarina; Vieira, Luís; Sousa, Cláudia Sofia CunhaThe increase of antibiotic resistance calls for alternatives to control Helicobacter pylori, a Gram-negative bacterium associated with various gastric diseases. Bacteriophages (phages) can be highly effective in the treatment of pathogenic bacteria. Here, we developed a method to identify prophages in H. pylori genomes aiming at their future use in therapy. A polymerase chain reaction (PCR)-based technique test...
Novas perspetivas sobre o papel e as aplicações dos bacteriófagos de Helicobact...
Ferreira, Rute Vanessa Novais; Oleastro, Mónica; Azeredo, Joana; Figueiredo, Céu; Melo, Luís Daniel RodriguesAs infeções por Helicobacter pylori são caracterizadas por uma elevada incidência global, constituindo uma ameaça significativa à saúde pública. Os tratamentos atuais envolvem a administração de antibióticos e inibidores da bomba de protões. No entanto, esta abordagem terapêutica apresenta uma eficácia limitada, principalmente devido ao aumento da resistência aos antibióticos em todo o mundo. Este cenário salie...
New insights into bacteriophages’ role and applications
Ferreira, Rute Vanessa NovaisHelicobacter pylori causes chronic gastric infection, affecting a significant proportion of the population worldwide. Current treatments rely on antibiotics but face reduced efficacy due to increasing antibiotic resistance, highlighting the urgent need for alternative therapies. Bacteriophages (phages) represent a promising option for the biocontrol of H. pylori. To date, only H. pylori prophages have been iden...
Advances on diagnosis of Helicobacter pylori infections
Sousa, Cláudia Sofia Cunha; Ferreira, Rute Vanessa Novais; Santos, Sílvio Roberto Branco; Azevedo, Nuno F.; Melo, Luís Daniel RodriguesThe association of Helicobacter pylori to several gastric diseases, such as chronic gastritis, peptic ulcer disease, and gastric cancer, and its high prevalence worldwide, raised the necessity to use methods for a proper and fast diagnosis and monitoring the pathogen eradication. Available diagnostic methods can be classified as invasive or non-invasive, and the selection of the best relies on the clinical cond...
Screening and in silico characterization of prophages in Helicobacter pylori ge...
Ferreira, Rute Vanessa Novais; Sousa, Cláudia Sofia Cunha; Presa, Eva; Pires, Diana Priscila Penso; Oleastro, Mónica; Azeredo, Joana; Figueiredo, CéuTemperate bacterio(phages) play an important role on the evolution of pathogenic bacteria. Nevertheless, information on their role in Helicobacter pylori (an important gastric pathogen bacterium) is scarce. The present study developed a workflow for the identification of prophages in Portuguese H. pylori clinical strains, proposing the use of a new PCR-based screening method. The genome of strains with differen...
Bacteriophages have potential to control foaming caused by Rhodococcus erythrop...
Ferreira, Rute Vanessa Novais; Santos, Sílvio Roberto Branco; Melo, Luís Daniel Rodrigues; Ferreira, Vânia; Mota, M.; Nicolau, AnaActivated-sludge is the most widely used biological process to remove pollutants from wastewater worldwide, mainly due to its economic advantages. Bacteria sum up around 95 % of the total microbial community of the activated-sludge, being responsible for most of the water depuration. Filamentous bacteria are normal components of these artificial ecosystems but their excessive growth leads to potential problems,...
H. pylori phages: from genome release to hope for use as therapy
Ferreira, Rute Vanessa Novais; Sousa, Cláudia Sofia Cunha; Presa, Eva; Pires, Diana Priscila Penso; Gonçalves, Raquel F. S.; Pinheiro, A. C.The increasing antibiotic-resistant Helicobacter pylori infections worldwide and the ineffectiveness of treatments led the World Health Organization to designate clarithromycin-resistant H. pylori as a high-priority bacterium for antibiotic research and development. (Bacterio)phages, viruses that infect bacteria, showing effectiveness in the treatment of pathogenic bacteria, could be a promising alternative str...
Helicobacter pylori infection: from standard to alternative treatment strategies
Sousa, Cláudia; Ferreira, Rute Vanessa Novais; Azevedo, Nuno F.; Oleastro, Mónica; Azeredo, Joana; Figueiredo, Céu; Melo, Luís Daniel RodriguesHelicobacter pylori is the major component of the gastric microbiome of infected individuals and one of the aetiological factors of chronic gastritis, peptic ulcer disease and gastric cancer. The increasing resistance to antibiotics worldwide has made the treatment of H. pylori infection a challenge. As a way to overhaul the efficacy of currently used H. pylori antibiotic-based eradication therapies, alternativ...
Characterization and genomic analysis of a new phage infecting Helicobacter pylori
Ferreira, Rute Vanessa Novais; Sousa, Cláudia Sofia Cunha; Gonçalves, Raquel; Pinheiro, A. C.; Oleastro, Mónica; Wagemans, Jeroen; Lavigne, RobHelicobacter pylori, a significant human gastric pathogen, has been demonstrating increased antibiotic resistance, causing difficulties in infection treatment. It is therefore important to develop alternatives or complementary approaches to antibiotics to tackle H. pylori infections, and (bacterio)phages have proven to be effective antibacterial agents. In this work, prophage isolation was attempted using H. py...
A novel Helicobacter pylori phage to combat gastric infections: first character...
Ferreira, Rute Vanessa Novais; Sousa, Cláudia Sofia Cunha; Gonçalves, Raquel F. S.; Pinheiro, A. C.; Oleastro, Mónica; Azeredo, Joana; Figueiredo, CéuHelicobacter pylori, a Gram-negative microaerophilic bacterium in a spiral shape, is recognized as an etiological agent of active chronic gastritis and contributes to peptic ulceration1. Furthermore, some studies associate it to development of gastric carcinoma2. Currently, treatment of H. pylori is performed with conventional antibiotics that in addition to the several side effects, increase the appearance of ...