14 documents found, page 1 of 2
Mexican calea (Calea zacatechichi Schltdl.) interferes with cholinergic and dop...
Garcia, Maria Rita; Gil-Izquierdo, Ángel; Ferreres, Federico; Andrade, Paula B.; Mineiro, Tiago; Seabra, Vítor; Videira, Romeu A.; Silva, Diana Dias daThe use of “Mexican calea” (Calea zacatechichi Schltdl.) in ritualistic ceremonies, due to its dream-inducing effects, was until recently limited to indigenous communities in Mexico. However, the plant has recently gained popularity in Western societies being commonly used in recreational settings. Despite the traditional and recreational uses, mechanisms underlying its reported oneirogenic effects remain unkno...
Chemical characterization and in vitro studies on the impact of the 'Dream Herb...
Garcia, Maria Rita; Mineiro, Tiago; Andrade, Paula B.; Ferreres, Federico; Gil-Izquierdo, Ángel; Seabra, Vítor; Dias-da-Silva, DianaBackground: An increasing number of psychoactive plants, particularly those with long history of use on ritualistic practices, are now being progressively used in recreational context, many of which remaining unregulated [1]. Calea zacatechichi is traditionally used in divination rituals, due to its oneirogenic properties. Yet, considering the psychoactive effects of this ‘Dream Herb’ and easiness of purchase, ...
Chemical characterization and in vitro studies on the impact of the 'Dream Herb...
Garcia, Maria Rita; Mineiro, Tiago; Andrade, Paula B.; Ferreres, Federico; Gil-Izquierdo, Ángel; Seabra, Vítor; Silva, Diana; Gomes, Nelson G. M.An increasing number of psychoactive plants, particularly those with long history of use on ritualistic practices, are now being progressively used in recreational context, many of which remaining unregulated. Calea zacatechichiis traditionally used in divination rituals, due to its oneirogenic properties. Yet, considering the psychoactive effects of this ‘Dream Herb’and easiness of purchase, the potential for ...
Structural/Functional Matches and Divergences of Phytoprostanes and Phytofurans...
Medina, Sonia; Gil-Izquierdo, Angel; Durand, Thierry; Ferreres, Federico; Domínguez-Perles, RaúlStructure-activity relationship (SAR) constitutes a crucial topic to discover new bioactive molecules. This approach initiates with the comparison of a target candidate with a molecule or a collection of molecules and their attributed biological functions to shed some light in the details of one or more SARs and subsequently using that information to outline valuable application of the newly identified compound...
Brassica oleracea L. Var. costata DC and Pieris brassicae L. aqueous extracts r...
Sousa, Carla; Fernandes, Fátima; Valentão, Patrícia; Rodrigues, António Sebastião; Coelho, Marta; Teixeira, João Paulo; Silva, SusanaBrassica oleracea L. var. costata DC leaves and Pieris brassicae L. larvae aqueous extracts were assayed for their potential to prevent/induce DNA damage. None of them was mutagenic at the tested concentrations in the Ames test reversion assay using Salmonella His+ TA98 strains, with and without metabolic activation. In the hypoxanthine-guanine phosphoribosyltransferase mutation assay using mammalian V79 fibrob...
Phlorotannin Extracts from Fucales Characterized by HPLC-DAD-ESI-MSn: Approache...
Ferreres, Federico; Lopes, Graciliana; Gil-Izquierdo, Angel; Andrade, Paula; Sousa, Carla; Mouga, Teresa; Valentão, PatríciaPurified phlorotannin extracts from four brown seaweeds (Cystoseira nodicaulis (Withering) M. Roberts, Cystoseira tamariscifolia (Hudson) Papenfuss, Cystoseira usneoides (Linnaeus) M. Roberts and Fucus spiralis Linnaeus), were characterized by HPLC-DAD-ESI-MSn . Fucophloroethol, fucodiphloroethol, fucotriphloroethol, 7-phloroeckol, phlorofucofuroeckol and bieckol/dieckol were identified. The antioxidant activit...
Sterol profiles in 18 macroalgae of the Portuguese coast
Lopes, Graciliana; Sousa, Carla; Bernardo, João; Andrade, Paula B.; Valentão, Patrícia; Ferreres, Federico; Mouga, TeresaThe sterol profiles of dominant macroalgae occur-ring in the western Portuguese coast were evaluated.An analytical procedure, involving alkaline hydrolysisand extraction followed by separation by reversed-phase HPLC–diode array detection (HPLC–DAD),was optimized for the study of their sterols composi-tion. The validated methodology is short in analysistime (as the compounds are determined in <20 min),sensitive,...
HPLC-PAD-atmospheric pressure chemical ionization-MS metabolite profiling of cy...
Ferreres, Federico; Pereira, David M.; Gil-Izquierdo, Angel; Valentão, Patrícia; Botelho, João; Mouga, Teresa; Andrade, Paula B.An HPLC-PAD-atmospheric pressure chemical ionization-MS metabolite profiling analysis was conducted on the marine echinoderm Marthasterias glacialis (spiny sea-star). Bio-guided purification of the methanolic extract led to the isolation of several carotenoids, namely zeaxanthin, astaxanthin and lutein. These compounds were characterized using both UV–Vis characteristics and MS spectra interpretation. No previo...
Induction of phenolic compounds in Hypericum perforatum L. cells by colletotric...
Conceição, Luís F. R.; Ferreres, Federico; Tavares, R. M.; Dias, Alberto Carlos PiresChanges in phenolic metabolism after elicitation with Colletotrichum gloeosporioides (CG) has been studied in Hypericum perforatum L. (HP) cell suspension cultures. Soluble phenolics were analysed by HPLC–DAD and HPLC–DAD–MS/MS. HP cultures elicited with the CG elicitor showed a significant increase in xanthone accumulation. Xanthone accumulation increased twelve fold when the cells were primed with methyl-jasm...
Phytochemical and antioxidant characterization of Hypericum perforatum alcoholi...
Silva, Bruno A.; Ferreres, Federico; Malva, João O.; Dias, Alberto C. P.The antioxidant potentials of a total ethanolic extract of Hypericum perforatum (TE) and fractions were evaluated and correlated with their phenolic contents. The extracts were fully characterised by HPLC-DAD-MS-MS. Kaempferol 3-rutinoside and rutin-acetyl were identified for the first time in TE extracts. The free radical-scavenging properties of TE (EC50=21 [mu]g dwb/ml) and fractions were studied using DPPH....