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. FIRST MOLECULAR DETECTION OF LEISHMANIA INFANTUM IN SERGENTOMYIA MINUTA (DIPT...

Pereira, S.; de Pita Pereira, D.; Araujo-Pereira, T.; Britto, C.; Costa-Rego, T.; Ferrolho, J.; Vilhena, M.; Rangel, E.F.; Vilela, ML.; Afonso, MO.

One Sergentomyia minuta female was positive for L. infantum infection and another for human blood as a meal source.


Aspetos bioecológicos de flebotomíneos (Diptera, Psychodidae), vetores de Leish...

Pereira, S.; Pita-Pereira, D.; Araujo Pereira, T.; Britto, C.; Ferrolho, J.; Vilhena, M.; Rangel, E.F.; Vilela, M.L.; Afonso, O.

Aspectos bioecológicos de flebotomíneos no Novo e Velho mundo: Brasil e POrtugal


Rhipicephalus bursa Sialotranscriptomic Response to Blood Feeding and Babesia o...

Antunes, S.; Couto, J.; Ferrolho, J.; Rodrigues, F.; Nobre, J.; Santos, A.S.; Santos-Silva, M.M.; de la Fuente, J.; Domingos, A.

Ticks are among the most prevalent blood-feeding arthropods, and they act as vectors and reservoirs for numerous pathogens. Sialotranscriptomic characterizations of tick responses to blood feeding and pathogen infections can offer new insights into the molecular interplay occurring at the tick-host-pathogen interface. In the present study, we aimed to identify and characterize Rhipicephalus bursa salivary gland...


Molecular heterogeneity of Rhipicephalus sanguineus sensu lato and screening fo...

Santos, G.S.; Couto, J.; Silva-Perdosa, R.; Ferrolho, J.; Santos, A.S.; Santos-Silva, M.M.; Antunes, S.; Domingos, A.

The present study aimed to expand knowledge regarding the molecular characterization of R. sanguineus s.l. in Portugal and to screen for ticks naturally infected with E. canis. A total of 113 R. sanguineus s.l. were collected questing or attached to domestic and wild animals from the 18 administrative regions of mainland Portugal. All the ticks were analyzed for the 16S rRNA and the partial sequences obtained s...


Ixodídeos (Acari:Ixodida) em animais de produção no distrito de Portalegre: det...

Velez, Rita; Santos, Ana Sofia; Antunes, Sandra; Ferrolho, J.; Bacellar, Daniel; Padre, Ludovina; Domingos, A.; Santos Silva, Margarida

A pesquisa de agentes infecciosos em vectores ixodídeos removidos de vertebrados permite-nos a sinalização de possíveis focos de infecção em animais assintomáticos. A manutenção em vectores e hospedeiros de agentes associados a carraças, alguns dos quais com carácter zoonótico, têm um impacto directo quer na saúde e produção animal, quer como um potencial risco para a Saúde Pública. Entre Novembro de 2012 e Jan...


PCR screening of tick-borne agents in sensitive conservation areas, Southeast P...

Santos-Silva, M.M.; Melo, P.; Santos, N.; Antunes, S.; Duarte, L.R.; Ferrolho, J.; Milhano, N.; Santos, P.T.; Domingos, A.; Santos, A.S.

The Southeast region of Portugal, particularly the Guadiana valley, is currently the reintroduction territory of Lynx pardinus (Iberian lynx), one of the most endangered felids in the world that is only found in the Iberian Peninsula. Over the last century, populations have declined, placing L. pardinus at extremely high risk of extinction in the wild and relying on reintroduction projects. Among the aspects ta...


An insight into the transcriptome of Rhipicephalus bursa: differential gene exp...

Couto, J.; Antunes, S.; Ferrolho, J.; Santos, A.S.; Santos-Silva, M.M.; de la Fuente, J.; Domingos, A.

Understanding the paradigm of the pathogen-tick-host triad remains an intricate issue. Basic research focusing on each one of these interplays is necessary to better comprehend this dynamic system. One of such complex association is the Babesia ovis, transmitted by the Rhipicephalus bursa ticks. B. ovis is an intra-erythrocytic protozoan parasite highly pathogenic parasite, causing severe infection in sheep. Pa...


Anaplasma marginale and Theileria annulata in questing ticks from Portugal

Antunes, S.; Ferrolho, J.; Domingues, N.; Santos, A.S.; Santos-Silva, M.M.; Domingos, A.

Ticks are ubiquitous arthropods and vectors of several pathogenic agents in animals and humans. Monitoring questing ticks is of great importance to ascertain the occurrence of pathogens and the potential vector species, offering an insight into the risk of disease transmission in a given area. In this study 428 host-seeking ticks, belonging to nine species of Ixodidae and collected from 17 of the 23 Portuguese ...


Vitellogenin and Lachesin knockdown in Rhipicephalus bursa influence in feeding...

Antunes, S.; Ferrolho, J.; Nobre, J.; Couto, J.; Santos, A.S.; Santos-Silva, M.M.; de la Fuente, J.; Domingos, A.

Rhipicephalus bursa is considered as a multi-host tick being the primary hosts of this species, cattle, sheep and goats. R. bursa is widely distributed in the Mediterranean region and has been described as being involved in the transmission of agents of the genus Anaplasma, Babesia, Theileria, Rickettsia among others, with a known impact on animal health. Control of ticks by vaccination has the advantages of be...


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