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First description of a fatal equine infection with Halicephalobus gingivalis in...

Noiva, Rute; Ruivo, Pedro; Madeira de Carvalho, Luís; Fonseca, Constança; Fevereiro, Miguel; Carvalho, Paulo; Orge, Leonor; Monteiro, Madalena

ABSTRACT - Halicephalobus gingivalisis a small saprophytic rhabditid nematode, represented only by females with a typicalrhabditoid oesophagus and one egg in the uterus, capable of infecting vertebrates. This opportunistic parasitepresent in the soil, manure and decaying humus, is thought to penetrate through previous injuries to the mouth,eyes and skin of horses and migrate to various organs. The brain is one ...


Emergence of rabbit haemorrhagic disease virus 2 in the archipelago of Madeira,...

Carvalho, Carina Luísa; Silva, Sara; Gouveia, Paz; Costa, Margarida; Duarte, Elsa Leclerc; Henriques, Ana Margarida; Barros, Sílvia Santos; Luís, Tiago

Emergence of rabbit haemorrhagic disease virus 2 in the archipelago of Madeira, Portugal (2016–2017)


Tracking the Origin of a Rabbit Haemorrhagic Virus 2 Outbreak in a Wild Rabbit ...

Carvalho, CL; Rodeia, J; Branco, Sandra; Monteiro, M; Duarte, EL; Melo, Pedro; Santos, Patricia Tavares; Henriques, Ana Margarida; Santos Barros, Silvia

As key prey, the wild rabbit downsize constitutes a major drawback on the endangered Iberian lynx (Lynx pardinus) re-introduction in the Iberia. Several captive breeding units mostly located in Alentejo, endeavour the wild rabbit repopulation of depleted areas assigned for the lynx re-introduction. Here we report an RHDV2 outbreak that occurred in early 2016 in a wild rabbit captive breeding unit located in Bar...


Tracking the Origin of a Rabbit Haemorrhagic Virus 2 Outbreak in a Wild Rabbit ...

Carvalho, CL; Rodeia, Joana; Branco, Sandra; Monteiro, Madalena; Duarte, Elsa Leclerc; Melo, Pedro; Tavares Santos, Patricia; Henriques, Ana Margarida

As key prey, the wild rabbit downsize constitutes a major drawback on the endangered Iberian lynx (Lynx pardinus) re-introduction in the Iberia. Several captive breeding units mostly located in Alentejo, endeavour the wild rabbit repopulation of depleted areas assigned for the lynx re-introduction. Here we report an RHDV2 outbreak that occurred in early 2016 in a wild rabbit captive breeding unit located in Bar...


Detection of virulence-associated genes in pathogenic and commensal avian Esche...

Paixão, Ana; Ferreira, A; Fontes, Miguel; Themudo, Patrícia; Albuquerque, Teresa; Chaveiro Soares, Manuel; Fevereiro, Miguel; Martins, Luís

Poultry colibacillosis due to Avian Pathogenic Escherichia coli (APEC) is responsible for several extra-intestinal pathological conditions, leading to serious economic damage in poultry production. The most commonly associated pathologies are airsacculitis, colisepticemia, and cellulitis in broiler chickens, and salpingitis and peritonitis in broiler breeders. In this work a total of 66 strains isolated from de...


A real time Taqman RT-PCR for the detection of rabbit hemorrhagic disease virus...

Duarte, Margarida Dias; Carvalho, Carina L.; Barros, Silvia C.; Henriques, Ana M.; Ramos, Fernanda; Fagulha, Teresa; Luís, Tiago; Duarte, Elsa L.

As key prey, the wild rabbit downsize constitutes a major drawback on the endangered Iberian lynx (Lynx pardinus) re-introduction in the Iberia. Several captive breeding units mostly located in Alentejo, endeavour the wild rabbit repopulation of depleted areas assigned for the lynx re-introduction. Here we report an RHDV2 outbreak that occurred in early 2016 in a wild rabbit captive breeding unit located in Bar...


A real time Taqman RT-PCR for the detection of rabbit hemorrhagic disease virus...

Duarte, Margarida Dias; Carvalho, Carina Luísa; Barros, Sílvia; Henriques, Ana M.; Ramos, Fernanda; Fagulha, Teresa; Luís, Tiago; Duarte, Elsa Leclerc

A specific real time RT-PCR for the detection of RHDV2 was developed and validated using RHDV and RHDV2 RNA preparations from positive field samples. The system was designed to amplify a 127 nucleotide-long RNA region located within the vp60 gene, based on the alignment of six sequences originated in Portugal, obtained in our laboratory, and 11 sequences from France and Italy. The primers and probe target seque...


Snapshot of viral infections in wild carnivores reveals ubiquity of parvovirus ...

Duarte, Margarida D.; Henriques, Ana Margarida; Barros, Sílvia Carla; Fagulha, Teresa; Mendonça, Paula; Carvalho, Paulo; Monteiro, Madalena

The exposure of wild carnivores to viral pathogens, with emphasis on parvovirus (CPV/FPLV), was assessed based on the molecular screening of tissue samples from 128 hunted or accidentally road-killed animals collected in Portugal from 2008 to 2011, including Egyptian mongoose (Herpestes ichneumon, n = 99), red fox (Vulpes vulpes, n = 19), stone marten (Martes foina, n = 3), common genet (Genetta genetta, n = 3)...


Serological evidence of West Nile virus circulation in Portugal

Barros C. S., Sílvia; Fevereiro, Miguel

The circulation of West Nile virus in Portugal was assessed by serological surveys conducted during 2004–2010 in horses and birds. The detection of WNV antibodies in both species in all the years covered by the study as well as the presence of anti-WNV IgM in symptomatic horses that had not traveled outside the country, support the notion that WNV circulates in Portugal.


Multiyear surveillance of Influenza A virus in wild birds in Portugal

Henriques, Ana Margarida; Fevereiro, Miguel

This report presents the results of a multiyear (2005 to 2009) study of avian influenza virus (AIV) occurrence in wild birds in Portugal. A total of 5691 samples from wild birds belonging to 13 different orders were examined. Ninety-three samples tested positive for AIV by matrix reverse transcriptase-polymerase chain reaction, giving a total prevalence of 1.63%. Twenty-one viruses were successfully cultured in...


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