9 documents found, page 1 of 1
Effects of Zn-ZnO Core-Shell Nanoparticles on Antimicrobial Mechanisms and Immu...
Fialho, Luísa; Costa-Barbosa, Augusto; Sampaio, Paula; Carvalho, SandraThe deposition of zinc-zinc oxide nanoparticles (Zn-ZnO NPs) onto porous Ta2O5 surfaces enriched with calcium phosphate by DC magnetron sputtering was investigated to improve the surface antimicrobial activity without triggering an inflammatory response. Different sizes and amounts of Zn NPs obtained by two optimized different depositions and an additional thin carbon (C) layer deposited over the NPs were explo...
cEpiderm, a Canine Skin Analog Suitable for In Vivo Testing Replacement
Marques, Mariana; Nunes, João; Ustymenko, Bárbara; Fialho, Luísa; Martins, Luís; Burke, Anthony; Filho, Cesar; Craveiro, Alexandre; Costa, AnaSkin is one of the organs most tested for toxicity and safety evaluation during the process of drug research and development and in the past has usually been performed in vivo using animals. Over the last few years, non-animal alternatives have been developed and validated epidermis models for human and rat skin are already available. Our goal was to develop a histotypical canine skin analog, suitable for non-a...
Development of a canine epidermis equivalent model for evaluation of sensitization
Marques, M; Nunes, J; Ustymenko, B; Lagoa, Tânia; Fialho, Luísa; Martins, Luís; Burke, Anthony; Souza, E; Filho, César; Craveiro, Alexandre; Costa, AnaBackground Canine atopic dermatitis (cAD) is a genetic-predisposed allergic and pruritic inflammatory skin condition, associated with sensitization to environmental allergens. Keratinocytes can produce several inflammatory mediators, in response to several other host mediators, antigens, and pathogens by virtue of their wide range of surface receptors, some of them dysregulated in cAD individuals. A histotypica...
A importância da Interleucina-33 como marcador da integridade da barreira cutân...
Paixão, Ana; Fialho, Luísa; Silva, Nídia; Martins, Luísobjectivo Um défice da barreira cutânea favorece a dermatite alérgica canina (DAc) e a interleucina (IL)-33, expressa em queratinócitos e fibroblastos, ativando células naive, linfócitos Th2, mastócitos, macrófagos, eosinófilos e células natural killer, e promovendo a diferenciação celular T helper (Th) 2, representa possível alvo terapêutico. O objetivo consistiu na avaliação da expressão de IL-33, em associaç...
How much does skin barrier count for allergic dermatitis improvement?
Martins, Luís; Fialho, Luísa; Caldeira, Joana; Sliva, Nídia; Antunes, Célia; Costa, Ana; Goicoa, Ana; Bento, OféliaBackground Allergic dermatitis is a genetic-based skin condition affecting an increasing number of dogs. A more efficient treatment approach is often multimodal, including a special focus on skin barrier condition, from which many pruritic triggers depend as well as common complications. Aim: To evaluate the specific role of skin barrier-directed reestablishing treatment in allergic dermatitis clinical improvem...
Morphology and oxygen incorporation effect on antimicrobial activity of silver ...
Rebelo, Rita; Manninen, N. K.; Fialho, Luísa; Henriques, Mariana; Carvalho, SandraAg and AgxO thin films were deposited by non-reactive and reactive pulsed DC magnetron sputtering, respectively, with the final propose of functionalizing the SS316L substrate with antibacterial properties. The coatings were characterized chemically, physically and structurally. The coatings nanostructure was assessed by X-ray diffraction (XRD) and X-ray photoelectron spectroscopy (XPS), while the coatings morp...
Estudo animal de próteses de anca em compósito PEEK-Carbono de anca
Reis, Joana; Potes, José; Fialho, Luísa; Cabrita, António; Torres Marques, António; Simões, José AntónioO estudo aqui apresentado teve como objectivo a avaliação de um compósito de PEEK reforçado em 30% com fibra de carbono curta, distribuída aleatoriamente, como material com potencial para aplicação ortopédica. Para o efeito, foram concebidos e fabricados protótipos de próteses de anca do tipo press-fit, baseadas em imagens radiográficas e implantadas em seis ovelhas. Dois dos animais foram sacrificados três mes...
Estudo in vitro da citotoxicidade do compósito de PEEK/ carbono
Reis, Joana; Potes, José; Fialho, Luísa; Marques, António Torres; Simões, José AntónioA polieteretercetona tem-se revelado como um material matricial prometedor devido às suas propriedades mecânicas, resistência química e possibilidade de esterilização por diversos métodos, incluindo autoclavagem.Diversos estudos têm também atestado características favoráveis de biocompatibilidade. O presente trabalho visou a avaliação de um compósito de PEEK reforçado com 30% de fibra de carbono, distribuída al...
Citotoxicity Study of Carbon-fibre Reinforced PEEK
Reis, Joana; Potes, José; Fialho, Luísa; Marques, António; Simões, JoséThe main goal within the present work was to test a 30% carbon fibre reinforced PEEK composite, commercially designated by KETRON® PEEK-CA30. The reinforcement of this type of composite material is randomly distributed with short fibres. The test and evaluation of biomaterial and osteogenic cells interaction was assessed by using tissue culture techniques that permit the isolation and growth of cellular populat...