9 documents found, page 1 of 1
Prevalência de conjuntivite adenoviral em clínica oftalmológica no município de...
Rosado-Filho, Euldes Nei; Cardoso, Silvia Almeida; Marçal, Lorena Nacif; Santos, Eliziária Cardoso dos; Silva, Eduardo de Almeida Marques daAvaliar a prevalência de Adenovírus como agente etiológico da conjuntivite, em clínica médica oftalmológica especializada, em Viçosa, Minas Gerais, Brasil. Amostras da secreção conjuntival de 91 pacientes clinicamente diagnosticados com conjuntivite foram submetidos à reação em cadeia da polimerase (PCR), utilizando primers degenerados para a região codificadora do gene da proteína estrutural II. Posteriormente...
The influence of ecto-nucleotidases on Leishmania amazonensis infection and imm...
Testasicca, Miriam Conceição de Souza; Assis, Elisângela Aparecida de; Gomes, Rodrigo Saar; Silva, Eduardo de Almeida Marques da; Melo, Maria NormaPrevious results from our laboratory and from the literature have implicated the expression of ectonucleotidases in the establishment of Leishmania infection. In the present study we evaluated the correlation between ecto-nucleotidasic activity and the infectivity of L. amazonensis promastigotes that were kept in culture for short or extended numbers of passages, a condition that is known to decrease parasite i...
Leishmania metacyclogenesis is promoted in the absence of purines.
Serafim, Tiago Donatelli; Figueiredo, Amanda Braga de; Costa, Pedro Augusto Carvalho; Silva, Eduardo de Almeida Marques da; Gonçalves, RicardoLeishmania parasites, the causative agent of leishmaniasis, are transmitted through the bite of an infected sand fly. Leishmania parasites present two basic forms known as promastigote and amastigote which, respectively, parasitizes the vector and the mammalian hosts. Infection of the vertebrate host is dependent on the development, in the vector, of metacyclic promastigotes, however, little is known about the ...
Antigenic extracts of Leishmania braziliensis and Leishmania amazonensis associ...
Grenfell, Rafaella Fortini Queiroz; Silva, Eduardo de Almeida Marques da; Testasicca, Miriam Conceição de Souza; Coelho, Eduardo Antônio FerrazThis study evaluated two vaccine candidates for their effectiveness in protecting BALB/c mice against Leishma¬nia chagasi infection. These immunogenic preparations were composed of Leishmania amazonensis or Leishmania braziliensis antigenic extracts in association with saponin adjuvant. Mice were given three subcutaneous doses of one of these vaccine candidates weekly for three weeks and four weeks later challe...
Evaluation of immune responses and protection induced by A2 and nucleoside hydr...
Zanin, Francisca Helena Calheiros; Coelho, Eduardo Antônio Ferraz; Tavares, Carlos Alberto Pereira; Silva, Eduardo de Almeida Marques daSeveral antigens have been tested as vaccine candidates against Leishmania infections but controversial results have been reported when different antigens are co-administered in combined vaccination protocols. Immunization with A2 or nucleoside hydrolase (NH) antigens was previously shown to induce Th1 immune responses and protection in BALB/c mice against Leishmania donovani and L. amazonensis (A2) or L. donov...
Successful vaccination against Leishmania chagasi infection in BALB/c mice with...
Vilela, Márcia de Carvalho; Gomes, Daniel Cláudio de Oliveira; Silva, Eduardo de Almeida Marques da; Serafim, Tiago DonatelliThis study evaluated the potential of a Leishmania antigen vaccine in protecting BALB/c mice against Leishmania chagasi. Mice received two subcutaneous doses of L. amazonensis vaccine with Corynebacterium parvum and subsequent boost was done without adjuvant. One week later, mice were challenged with L. chagasi. We observed that this vaccine caused a significant reduction in parasite load in liver and spleen an...
Influência do metabolismo extracelular de ATP em infecções por parasitos do gên...
Silva, Eduardo de Almeida Marques daVariações nos quadros clínicos da leishmaniose, observadas tanto na doença em humanos quanto em murinos, sugerem a existência de uma diferenciação, dependente da espécie, na capacidade de parasitos do gênero Leishmania causarem lesões no hospedeiro. A procura de fatores que diferenciam as espécies de Leishmania quanto à sua virulência se faz, então, necessária para o entendimento dos mecanismos pelos quais esse...
Extracellular nucleotide metabolism in Leishmania : influence of adenosine in t...
Silva, Eduardo de Almeida Marques da; Oliveira, Jamile Camargos de; Figueiredo, Amanda Braga de; Lima Júnior, Djalma de Souza; Carneiro, Cláudia MartinsLeishmaniasis is a parasitic disease with a variety of clinical forms, which are related to the Leishmania species involved. In the murine model, Leishmania amazonensis causes chronic non-healing lesions in Leishmania braziliensis- or Leishmania major-resistant mouse strains. In this study, we investigated the involvement of the pathway of extracellular nucleotide hydrolysis, with special focus on the role of e...
Development of chronic cardiomyopathy in canine Chagas disease correlates with ...
Guedes, Paulo Marcos da Matta; Veloso, Vanja Maria; Afonso, Luís Carlos Crocco; Caliari, Marcelo Vidigal; Carneiro, Cláudia MartinsWhen infected with Trypanosoma cruzi, Beagle dogs develop symptoms similar to those of Chagas disease in human beings, and could be an important experimental model for a better understanding of the immunopathogenic mechanisms involved in chronic chagasic infection. This study evaluates IL-10, IFN-g and TNF-a production in the sera, culture supernatant, heart and cervical lymph nodes and their correlation with c...