5 documents found, page 1 of 1
Screening and in silico characterization of prophages in Helicobacter pylori cl...
Ferreira, Rute Vanessa Novais; Pinto, Graça; Presa, Eva; Oleastro, Mónica; Silva, Catarina; Vieira, Luís; Sousa, Cláudia Sofia CunhaThe increase of antibiotic resistance calls for alternatives to control Helicobacter pylori, a Gram-negative bacterium associated with various gastric diseases. Bacteriophages (phages) can be highly effective in the treatment of pathogenic bacteria. Here, we developed a method to identify prophages in H. pylori genomes aiming at their future use in therapy. A polymerase chain reaction (PCR)-based technique test...
Helicobacter pylori infection: from standard to alternative treatment strategies
Sousa, Cláudia; Ferreira, Rute; Azevedo, Nuno F.; Oleastro, Mónica; Azeredo, Joana; Figueiredo, Ceu; Melo, Luís D.R.Helicobacter pylori is the major component of the gastric microbiome of infected individuals and one of the aetiological factors of chronic gastritis, peptic ulcer disease and gastric cancer. The increasing resistance to antibiotics worldwide has made the treatment of H. pylori infection a challenge. As a way to overhaul the efficacy of currently used H. pylori antibiotic-based eradication therapies, alternativ...
Characterization and Genomic Analysis of a New Phage Infecting Helicobacter pylori
Ferreira, Rute; Sousa, Cláudia; Gonçalves, Raquel F.S.; Pinheiro, Ana Cristina; Oleastro, Mónica; Wagemans, Jeroen; Lavigne, Rob; Figueiredo, CeuHelicobacter pylori, a significant human gastric pathogen, has been demonstrating increased antibiotic resistance, causing difficulties in infection treatment. It is therefore important to develop alternatives or complementary approaches to antibiotics to tackle H. pylori infections, and (bacterio) phages have proven to be effective antibacterial agents. In this work, prophage isolation was attempted using H. p...
Characterization and genomic analysis of a new phage infecting Helicobacter pylori
Ferreira, Rute Vanessa Novais; Sousa, Cláudia Sofia Cunha; Gonçalves, Raquel; Pinheiro, A. C.; Oleastro, Mónica; Wagemans, Jeroen; Lavigne, RobHelicobacter pylori, a significant human gastric pathogen, has been demonstrating increased antibiotic resistance, causing difficulties in infection treatment. It is therefore important to develop alternatives or complementary approaches to antibiotics to tackle H. pylori infections, and (bacterio)phages have proven to be effective antibacterial agents. In this work, prophage isolation was attempted using H. py...
Docosahexaenoic acid inhibits Helicobacter pylori growth in vitro and mice gast...
Correia, Marta; Michel, Valérie; Matos, António A.; Carvalho, Patrícia; Oliveira, Maria J.; Ferreira, Rui M.; Dillies, Marie-Agnès; Huerre, MichelH. pylori drug-resistant strains and non-compliance to therapy are the major causes of H. pylori eradication failure. For some bacterial species it has been demonstrated that fatty acids have a growth inhibitory effect. Our main aim was to assess the ability of docosahexaenoic acid (DHA) to inhibit H. pylori growth both in vitro and in a mouse model. The effectiveness of standard therapy (ST) in combination wit...