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Effect of coconut water and Braun-Collins solutions at different temperatures a...
Silva, J.R.V.; Lucci, Carolina Madeira; Carvalho, F.C.A.; Báo, Sônia Nair; Costa, S.H.F.; Santos, R.R.; Figueiredo, José RicardoPreservation of preantral follicles becomes very important to ensure follicle quality at the onset of cryopreservation or in vitro culture. However, for domestic animals, the ovarian donor of preantral follicles for in vitro studies is commonly encountered far away from reproduction laboratories. We investigated the effectiveness of coconut water and Braun- Collins solutions on the preservation of goat preantra...
Morphological and ultrastructural changes occurring during degeneration of goat...
Silva, José Roberto Viana; Báo, Sônia Nair; Lucci, Carolina Madeira; Carvalho, F.C.A.; Andrade, Evelyn Rabelo; Ferreira, M.A.L; Figueiredo, José RicardoThe present work has investigated the morphological and ultrastructural changes occurring during degeneration of goat preantral follicles preserved in vitro and showed quantitative data about the distribution of follicular degeneration types in the control and after preservation in coconut water solution or Braun–Collins solution at different temperatures (4, 20 or 39°C) and incubation times (4, 12 or 24 h). At...
Effect of the interval of serial sections of ovarian tissue in the tissue chopp...
Lucci, Carolina Madeira; Amorim, Christiani Andrade; Báo, Sônia Nair; Figueiredo, José Ricardo; Rodrigues, Ana Paula Ribeiro; Silva, José Roberto VianaThe present work investigated the effect of the interval of serial sections of ovarian tissue on the number of isolated preantral follicles in the goat. Goat ovaries were cut in the tissue chopper at eight different intervals. The quality of isolated follicles were evaluated by histology and transmission electron microscopy. Best results were obtained when the ovaries were cut in the tissue chopper at intervals...
Study of preantral follicle population in situ and after mechanical isolation f...
Lucci, Carolina Madeira; Amorim, Christiani Andrade; Rodrigues, Ana Paula Ribeiro; Figueiredo, José Ricardo; Báo, Sônia NairThe purposes of this study were to estimate the population of caprine preantral follicles, and to evaluate quantitatively and qualitatively the efficiency of a specific mechanical method for the isolation of preantral follicles from mixed breed goats at different reproductive stages. On average, 37,646±4277 preantral follicles were present in goat ovaries, and 13,631±2399 preantral follicles were obtained after...
Effect of Braun-Collins and Saline solutions at different temperatures and incu...
Carvalho, Fabíola Christian Almeida de; Lucci, Carolina Madeira; Silva, José Roberto Viana; Andrade, Evelyn Rabelo; Báo, Sônia NairThe present work has investigated the efficiency of Braun-Collins and Saline (0.9%) solutions in the conservation of goat preantral follicles in situ, at different temperatures and incubation times. For each animal the ovarian pair was divided into 19 fragments. One ovarian fragment was taken randomly and immediately fixed (control). The other 18 ovarian fragments were randomly distributed in tubes containing B...
Ultrastructure of sheep primordial follicles cultured in the presence of indol ...
Andrade, Evelyn Rabelo; Maddox-Hyttel, Poul; Landim-Alvarenga, Fernanda Da Cruz [UNESP]; Viana Silva, José Roberto; Alfieri, Amauri AlcindoSubmitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-27T11:26:16Z No. of bitstreams: 0Bitstream added on 2014-05-27T14:41:59Z : No. of bitstreams: 1 2-s2.0-80655124211.pdf: 2114911 bytes, checksum: a34ecd6ca76da83394634615e86068fa (MD5); Made available in DSpace on 2014-05-27T11:26:16Z (GMT). No. of bitstreams: 0 Previous issue date: 2011-12-01; The aim of this study was to inves...