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The human follicle stimulating hormone (hFSH) keeps the normal ultrastructure o...
Costa, Sanely Lourenço da; Costa, Eduardo Paulino da; Pereira, Emílio César Martins; Benjamin, Laércio dos Anjos; Verde, Isabel Bezerra LimaO objetivo desse estudo foi investigar os efeitos do FSH humano (hFSH) no cultivo in vitro de folículos pré-antrais caprinos. Fragmentos do córtex ovariano de caprinos foram cultivados em ?-MEM+ suplementado com 0, 10, ou 50 ng/mL de hFSH por um ou sete dias. Pequenos fragmentos de tecido ovariano cultivado e não cultivados, foram processados por histologia clássica e microscopia eletrônica de transmissão. Os t...
Bone Morphogenetic Protein-6 (BMP-6) induces atresia in goat primordial follicl...
Araújo, Valdevane Rocha; Lima-Verde, Isabel Bezerra; Name, Khesller Patrícia Olázia; Báo, Sônia Nair; Campello, Claudio CabralO presente estudo investigou os efeitos da proteína morfogenética óssea-6 (BMP-6) no desenvolvimento in vitro de folículos primordiais caprinos. Amostras de córtex ovariano de cabras foram cultivados por 1 ou 7 dias em Meio Essencial Mínimo (meio controle) suplementado com diferentes concentrações de BMP-6. As taxas de sobrevivência, ativação e crescimento foram avaliadas por histologia clássica e microscopia e...
Degeneration rate of preantral follicles in the ovaries of goats
Silva, José Roberto Viana; Ferreira, Marcos Antônio Leal; Costa, Sonia Helena Furtado; Santos, R.R; Carvalho, Fabíola Christian Almeida deThe degeneration rate of ovarian preantral follicles in goats, and the distribution in the follicular classes (primordial, primary or secondary) was assessed. Ovaries from adult goats were collected at a local slaughterhouse. To evaluate the morphology of the caprine preantral follicles in situ, one fragment from each ovary was fixed individually in Carnoy for 12 h, sectioned serially at a thickness of 7 μm and...
Zebu (Bos indicus) ovarian preantral follicles : morphological characterization...
Lucci, Carolina Madeira; Rumpf, Rodolfo; Figueiredo, José Ricardo de; Báo, Sônia NairPreantral follicles are a major source of oocytes, and their utilization as an important tool to store large number of female gametes for future use in reproductive programs has been investigated. The increasing importance of studies in this subject, together with the important role of Zebu cattle in the economy of tropical and subtropical countries as well as their well-known differences from European cattle, ...
Light microscopical and ultrastructural characterization of goat preantral foll...
Lucci, Carolina Madeira; Silva, José Roberto Viana; Carvalho, Fabíola Christian Almeida de; Figueiredo, José Ricardo de; Báo, Sônia NairGoat ovarian preantral follicles were morphologically and ultrastructurally described in this work. Primordial follicles are oocytes surrounded by one layer of squamous or squamouscuboidal granulosa cells; primary follicles have a single layer of cuboidal granulosa cells, and secondary follicles are oocytes surrounded by two or more layers of cuboidal granulosa cells. At all developmental stages a thick layer o...
Ultrastructural features of agouti (Dasyprocta aguti) preantral follicles cryop...
Wanderleya, Livia Schell; Luza, Hiédely Kenia Machado; Faustino, Luciana Rocha; Lima, Isadora Machado Teixeira; Lopes, Cláudio Afonso PinhoThe objective was to develop an efficient protocol for cryopreservation of agouti (Dasyprocta aguti) ovarian tissue. Agouti ovarian fragments were placed, for 10 min, in a solution containing MEM and fetal bovine serum plus 1.5 M dimethyl sulfoxide (DMSO), ethylene glycol (EG) or propanediol (PROH); some of those fragments were subsequently cryopreserved in a programmable freezer. After exposure and/or thawing,...
Transforming growth factor-β (TGF-β) maintains follicular ultrastructure and st...
Rodrigues, G.Q.; Lima, I.M.T.; Chaves, R.N.; Rossetto, R.; Costa, S.L.; Castro, S.V.; Barros, V.R.P.; Lopes, C.A.P.; Báo, Sônia NairO objetivo desse estudo foi investigar se o TGF-β afeta a sobrevivência, ativação e crescimento de folículos primordiais caprinos inclusos no córtex ovariano após o cultivo in vitro. Ovários de cabras foram coletados em abatedouro e fragmentos de tecido ovariano foram cultivados por um e sete dias em meio essencial mínimo alfa (α-MEM+) sozinho ou suplementado com TGF-β (1, 5, 10 ou 50ng/mL). Fragmentos ovariano...
Progesterone and follicle stimulating hormone interact and promote goat preantr...
Lima-Verde, Isabel Bezerra; Matos, Maria Helena Tavares de; Celestino, Juliana Jales de Hollanda; Rossetto, Rafael; Name, Khesller Patrícia OláziaEste trabalho veri_icou os efeitos da progesterona e do hormonio foliculo-estimulante (FSH) na sobrevivencia e no crescimento de foliculos pre-antrais caprinos. Fragmentos de tecido ovariano foram cultivados por 1 ou 7 dias em Meio Essencial Minimo (MEM) sozinho ou contendo progesterona (1, 2.5, 5, 10 ou 20ng/mL), FSH (50ng/mL) ou a combinacao entre esses dois hormonios. O tecido fresco (controle nao-cultivado)...
Effect of sequential medium on in vitro culture of goat ovarian cortical tissue
Magalhães-Padilha, Deborah de Melo; Andrade, P.M.; Sales, E.T.; Araújo, Valdevane Rocha; Lima, I.M.T.; Castro, S.V.; Faustino, L.; Lopes, C.A.PA sequential medium was evaluated on the survival, activation and growth rates of caprine preantral follicles submitted to a long-term culture period, aiming to establish an ideal in vitro culture system. Ovarian fragments were cultured for 16 days in α-MEM+ alone or supplemented with hormones (GH and/or FSH) added sequentially on different days of culture. Ovarian fragments were cultured in the first (days 0–8...
Cryopreservation of caprine ovarian tissue using glycerol and ethylene glycol
Rodrigues, Ana Paula Ribeiro; Amorim, C.A; Costa, Sonia Helena Furtado; Matos, Maria Helena Tavares de; Santos, R.R.; Lucci, Carolina MadeiraCryopreservation of ovarian tissue may be a potential alternative for the conservation of genetically superior animals, including high milk- and meat-producing goat breeds. However, until now, no information was available concerning the cryopreservation of preantral follicles (PF) enclosed in caprine ovarian tissue. The objective of the present study was to evaluate the structural and ultrastructural characteri...