3 documents found, page 1 of 1
Cystic adenomatoid malformations are induced by localized FGF10 overexpression ...
Gonzaga, Sílvia; Henriques-Coelho, Tiago; Davey, Marcus; Zoltick, Philip W.; Leite-Moreira, Adelino F.; Correia-Pinto, Jorge; Flake, Alan W.Fibroblast growth factor-10 (FGF10) is a mesenchymal growth factor, involved in epithelial and mesenchymal interactions during lung branching morphogenesis. In the present work, FGF10 overexpression was transiently induced in a temporally and spatially restricted manner, during the pseudoglandular or canalicular stages of rat lung development, by trans-uterine ultrasound-guided intraparenchymal microinjections ...
Targeted gene transfer to fetal rat lung interstitium by ultrasound-guided intr...
Henriques-Coelho, Tiago; Gonzaga, Sílvia; Endo, Masayuki; Zoltick, Philip W.; Davey, Marcus; Leite-Moreira, Adelino F.; Correia-Pinto, JorgeIn utero gene transfer to the developing lung may have clinical or research applications. In this study, we developed a new method for specifically targeting the fetal rat lung with adeno and lentiviral vectors encoding the enhanced green fluorescence protein (EGFP) marker gene at E15.5 using ultrasound biomicroscopy (UBM). Survival rate, morphometric parameters, viral biodistribution, and lung transduction eff...
Antenatal vitamin A administration attenuates lung hypoplasia by interfering wi...
Baptista, Maria J.; Rocha, Gustavo Filipe Melo Alves; Pedrosa, Carla; Gonzaga, Sílvia; Teles, Antónia; Estevão-Costa, José; Areias, José C.Early and late lung underdevelopment in congenital diaphragmatic hernia (CDH) is likely caused by nonmechanical (directly mediated by nitrofen) and mechanical (mediated by thoracic herniation) factors, respectively. The authors investigated if vitamin A enhances lung growth because of effects on both early and late determinants of lung hypoplasia. Background/Purpose Early and late lung underdevelopment in conge...