27 documents found, page 1 of 3
Identification and in silico analysis of the Citrus HSP70 molecular chaperone g...
Fietto, Luciano G.; Costa, Maximiller D. L.; Cruz, Cosme D.; Souza, Alessandra A.; Machado, Marcos A.; Fontes, Elizabeth P. B.The completion of the genome sequencing of the Arabidopsis thaliana model system provided a powerful molecular tool for comparative analysis of gene families present in the genome of economically relevant plant species. In this investigation, we used the sequences of the Arabidopsis Hsp70 gene family to identify and annotate the Citrus Hsp70 genes represented in the CitEST database. Based on sequence comparison...
Revisiting the soybean GmNAC superfamily
Melo, Bruno P.; Fraga, Otto T.; Silva, José Cleydson F.; Ferreira, Dalton O.; Brustolini, Otávio J. B.; Carpinetti, Paola A.; Machado, Joao Paulo B.The NAC (NAM, ATAF, and CUC) genes encode transcription factors involved with the control of plant morph-physiology and stress responses. The release of the last soybean (Glycine max) genome assembly (Wm82.a2.v1) raised the possibility that new NAC genes would be present in the soybean genome. Here, we interrogated the last version of the soybean genome against a conserved NAC domain structure. Our analysis ide...
Repeat variants for the SbMATE transporter protect sorghum roots from aluminum ...
Martins, Laura G. C.; Pimenta, Maiana R.; Duarte, Christiane E. M.; Fontes, Elizabeth P. B.; Melo, Janaina O.; Barros, Beatriz A.; Lana, Ubiraci G. P.Acidic soils, where aluminum (Al) toxicity is a major agricultural constraint, are globally widespread and are prevalent in developing countries. In sorghum, the root citrate transporter SbMATE confers Al tolerance by protecting root apices from toxic Al3+, but can exhibit reduced expression when introgressed into different lines. We show that allele-specific SbMATE transactivation occurs and is caused by facto...
A WW domain-containing protein forms immune nuclear bodies against begomoviruses
Calil, Iara P.; Quadros, Iana P. S.; Araújo, Thais C.; Duarte, Christiane E. M.; Gouveia-Mageste, Bianca C.; Silva, José Cleydson F.The bipartite begomoviruses (Geminiviridae family), which are DNA viruses that replicate in the nucleus of infected cells, encode the nuclear shuttle protein (NSP) to facilitate the translocation of viral DNA from the nucleus to the cytoplasm via nuclear pores. This intracellular trafficking of NSP–DNA complexes is accessorized by the NSP-interacting guanosine triphosphatase (NIG) at the cytosolic side. Here, w...
Genetic diversity of begomovirus infecting tomato and associated weeds in south...
Ambrozevicius, Luciana P.; Calegario, Renata F.; Fontes, Elizabeth P. B.; Carvalho, Murilo G. de; Zerbini, F. MuriloA análise da variabilidade genética de geminivírus infetando tomateiros (Lycopersicon esculentum) na região Sudeste do Brasil foi realizada por meio do seqüenciamento direto de fragmentos de PCR amplificados com oligonucleotídeos universais para o componente A de begomovírus, seguido de análise filogenética. Amostras de tomateiro e de algumas plantas daninhas associadas, apresentando sintomas típicos de infecçã...
NIK1, a host factor specialized in antiviral defense or a novel general regulat...
Machado, Joao P. B.; Brustolini, Otavio J. B.; Mendes, Giselle C.; Santos, Anésia A.; Fontes, Elizabeth P. B.NIK1 is a receptor‐like kinase involved in plant antiviral immunity. Although NIK1 is structurally similar to the plant immune factor BAK1, which is a key regulator in plant immunity to bacterial pathogens, the NIK1‐mediated defenses do not resemble BAK1 signaling cascades. The underlying mechanism for NIK1 antiviral immunity has recently been uncovered. NIK1 activation mediates the translocation of RPL10 to th...
Distinct repressing modules on the distal region of the SBP2 promoter contribut...
Freitas, Rejane L.; Carvalho, Claudine M.; Fietto, Luciano G.; Loureiro, Marcelo E.; Almeida, Andrea M.; Fontes, Elizabeth P. B.The Glycine max sucrose binding protein (GmSBP2) promoter directs vascular tissue-specific expression of reporter genes in transgenic tobacco. Here we showed that an SBP2-GFP fusion protein under the control of the GmSBP2 promoter accumulates in the vascular tissues of vegetative organs, which is consistent with the proposed involvement of SBP in sucrose transport-dependent physiological processes. Through gain...
A PERK-Like receptor Kinase interacts with the geminivirus nuclear shuttle prot...
Florentino, Lilian H.; Santos, Anésia A.; Fontenelle, Mariana R.; Pinheiro, Guilherme L.; Zerbini, Francisco M.; Baracat-Pereira, Maria C.The nuclear shuttle protein (NSP) from bipartite geminiviruses facilitates the intracellular transport of viral DNA from the nucleus to the cytoplasm and acts in concert with the movement protein (MP) to promote the cell-to-cell spread of the viral DNA. A proline-rich extensin-like receptor protein kinase (PERK) was found to interact specifically with NSP of Cabbage leaf curl virus (CaLCuV) and of tomato-infect...
NIK1-mediated translation suppression functions as a plant antiviral immunity m...
Zorzatto, Cristiane; Machado, João Paulo B.; Lopes, Kênia V. G.; Nascimento, Kelly J. T.; Pereira, Welison A.; Brustolini, Otávio J. B.Plants and plant pathogens are subject to continuous co-evolutionary pressure for dominance, and the outcomes of these interactions can substantially impact agriculture and food security^ 1–3 . In virus– plant interactions, one of the major mechanisms for plant antiviral immunity relies on RNA silencing, which is often suppressed by co-evolving virus suppressors, thus enhancing viral pathogenicity in susceptibl...
The phosphorylation state and expression of soybean BiP isoforms are differenti...
Cascardo, Júlio Cézar M.; Almeida, Raul S.; Buzeli, Reginaldo A. A.; Carolino, Sônia M. B.; Otoni, Wagner C.; Fontes, Elizabeth P. B.The mammalian BiP is regulated by phosphorylation, and it is generally accepted that its unmodified form constitutes the biologically active species. In fact, the glycosylation inhibitor tunicamycin induces dephosphorylation of mammalian BiP. The stress-induced phosphorylation state of plant BiP has not been examined. Here, we demonstrated that soybean BiP exists in interconvertible phosphorylated and nonphosph...