60 documents found, page 1 of 6
In vitro activity of six antiviral drugs against equid alphaherpesvirus type 1 ...
Oliveira,Amanda Lovato de; Cargnelutti,Juliana Felipetto; Mortari,Ana Paula Gnocato; Flores,Eduardo Furtado; Weiblen,RudiABSTRACT: Equid alphaherpesvirus type 1 (EHV-1) is distributed worldwide and is a major agent of abortion, respiratory and neurological disease in horses. No specific treatment is available for EHV-1 infection, yet the potential of antiviral therapy has been explored. In this study we investigated the in vitro activity of Acyclovir, Ganciclovir, Foscarnet, Famciclovir, Vidarabina and Cidofovir against EHV-1. Fo...
Serological response to rabies virus induced by commercial vaccines in cattle
Martins,Mathias; Quadros,João Motta de; Flores,Eduardo Furtado; Weiblen,RudiABSTRACT: The antibody response to rabies virus (RABV) induced by commercial vaccines in heifers was investigated. For this, 84 heifers were vaccinated twice (30 days interval) with each of four vaccines (G1 = 14 animals; G2 = 24; G3 = 22 and G4 = 24) and received a booster vaccination 360 days later. Serum samples collected at different intervals after vaccination and 30 days after booster were submitted to a ...
Antibodies against rabies virus in dogs with and without history of vaccination...
Fernandes,Karina Gonzalez; Martins,Mathias; Amaral,Bruna Portolan; Cargnelutti,Juliana Felipetto; Weiblen,Rudi; Flores,Eduardo FurtadoABSTRACT: The present study investigated the frequency and magnitude of neutralizing antibodies to rabies virus (RABV) in dogs with and without historic of vaccination in Santa Maria/RS. Group A included serum samples from 440 dogs with recent historic of vaccination against rabies, obtained during the 2015 rabies vaccination campaign. Group B included 300 serum samples from dogs submitted to the Veterinary Hos...
Experimental infection of horses with Vaccinia virus
Barbosa,Claudio Henrique Gonçalves; Sant'Ana,Fabiano José Ferreira de; Cargnelutti,Juliana Felipetto; Flores,Eduardo FurtadoABSTRACT: Vaccinia virus (VACV) is the etiologic agent of bovine vaccinia, an emerging zoonotic disease with potential health issues for dairy herds and humans. VACV may occasionally infect other species, including horses. In this sense, an outbreak of VACV disease in horses was described in Pelotas, RS, in 2008, where a co-infection with two VACV strains (named Pelotas Virus 1 [P1V] and Pelotas Virus 2 [P2V]) ...
Antibodies against vesicular stomatitis virus in horses from southern, midweste...
Lunkes,Vinícius Leobet; Tonin,Alexandre Alberto; Machado,Gustavo; Corbellini,Luis Gustavo; Diehl,Gustavo Nogueira; Santos,Lucila Carboneiro dosABSTRACT: Vesicular stomatitis virus (VSV) is the agent of a vesicular disease that affects many animal species and may be clinically confounded with foot-and-mouth disease in ruminant and swine. Horses are especially susceptible to VSV and may serve as sentinels for virus circulation. The present study investigated the presence of neutralizing antibodies against VSV Indiana III (VSIV-3) in serum samples of 3,6...
Detection of respiratory viruses in shelter dogs maintained under varying envir...
Monteiro,Francielle Liz; Cargnelutti,Juliana Felipetto; Martins,Mathias; Anziliero,Deniz; Erhardt,Magnólia Martins; Weiblen,Rudi; Flores,Eduardo FurtadoAbstract Three dog shelters in Rio Grande do Sul were investigated for associations between the occurrence of respiratory viruses and shelter environmental conditions. Nasal secretions randomly collected during the cold season were tested via PCR, and this data collection was followed by nucleotide sequencing of the amplicons. In shelter #1 (poor sanitary and nutritional conditions, high animal density and cons...
Use of homologous recombination in yeast to create chimeric bovine viral diarrh...
Arenhart,Sandra; Silva Junior,José Valter Joaquim; Flores,Eduardo Furtado; Weiblen,Rudi; Gil,Laura Helena Vega GonzalesAbstract The open reading frame of a Brazilian bovine viral diarrhea virus (BVDV) strain, IBSP4ncp, was recombined with the untranslated regions of the reference NADL strain by homologous recombination in Saccharomyces cerevisiae, resulting in chimeric full-length cDNA clones of BVDV (chi-NADL/IBSP4ncp#2 and chi-NADL/IBSP4ncp#3). The recombinant clones were successfully recovered, resulting in viable viruses, h...
Safety and immunogenicity of a glycoprotein E gene-deleted bovine herpesvirus 1...
Weiss,Marcelo; Anziliero,Deniz; Martins,Mathias; Weiblen,Rudi; Flores,Eduardo FurtadoABSTRACT: A glycoprotein E-deleted Brazilian bovine herpesvirus 1 (BoHV-1gEΔ) was tested regarding to safety and immunogenicity. Intramuscular inoculation of young calves with a high virus dose did not result in clinical signs or virus shedding during acute infection or after dexamethasone administration. Calves vaccinated once IM (group I) or subcutaneously (group II) with live BoHV-1gEΔ or twice with inactiva...
Resposta sorológica aos herpesvirus bovino tipos 1 e 5 e vírus da diarreia vira...
Anziliero,Deniz; Martins,Mathias; Weiss,Marcelo; Monteiro,Francielle Liz; Ataide,Cássio Frustenal; Weiblen,Rudi; Flores,Eduardo FurtadoEste trabalho avaliou a imunogenicidade de vacinas para os herpesvírus bovino 1 e 5 (BoHV-1, BoHV-5) e vírus da diarreia viral bovina 1 e 2 (BVDV-1, BVDV-2), disponíveis no mercado brasileiro. Para isso, novilhos de raças de corte foram alocados em grupos de 10-12 animais e vacinados duas vezes, com intervalo de 30 dias, com cada uma das oito vacinas disponíveis. Amostras de soro coletadas 30 dias após a segund...
Virus isolation in cell culture for confirmatory diagnostic of rabies in bovine...
Kanitz,Fabio Adriano; Cargnelutti,Juliana Felipetto; Weiblen,Rudi; Batista,Helena Beatriz de Carvalho Ruthner; Flores,Eduardo FurtadoABSTRACT: This study investigated the suitability of virus isolation (VI) in mouse neuroblastoma cells (N2A) and baby hamster kidney cells (BHK-21) as a confirmatory test for diagnosis of bovine rabies. Fourty-eight brain samples from cattle suspected of rabies were initially submitted to fluorescent antibody test (FAT) and mouse inoculation test (MIT) for routine diagnostic. Subsequently, these specimens were ...