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Yarrowia lipolytica growth, lipids, and protease production in medium with high...
Miranda, Sílvia M.; Belo, Isabel; Lopes, MarleneTwo strains of Yarrowia lipolytica (CBS 2075 and DSM 8218) were first studied in bioreactor batch cultures, under different controlled dissolved oxygen concentrations (DOC), to assess their ability to assimilate aliphatic hydrocarbons (HC) as a carbon source in a mixture containing 2 g·L1 of each alkane (dodecane and hexadecane), and 2 g·L1 hexadecene. Both strains grew in the HC mixture without a lag phase, an...
Unraveling the potential of Yarrowia lipolytica to utilize wastemotor oil as a ...
Miranda, Sílvia M.; Belo, Isabel; Lopes, MarleneThis study evaluated the potential of Y. lipolytica (CBS 2075 and DSM 8218) to grow in waste motor oil (WMO) and produce valuable compounds, laying the foundation for a sustainable approach to WMO management. Firstly, yeast strains were screened for their growth on WMO (210 g·L1) in microplate cultures. Despite limited growth, the CBS 2075 strain exhibited comparable growth to control conditions (without WMO), ...
Yarrowia lipolytica is a promising oleaginous yeast for bio-oils production fro...
Pereira, Ana S.; Miranda, Sílvia M.; Belo, Isabel; Lopes, MarleneEvery year, approximately 931 million tons of food waste (FW) are generated worldwide. The discharge of FW in the landfill has a negative environmental impact due to water and soil pollution and GHG emission. Volatile fatty acids (VFAs), produced by anaerobic fermentation of FW, can be used as a substrate by oleaginous yeasts to produce bio-oils. The bioconversion of pure VFAs into Y. lipolytica biomass and bio...
Microbial valorization of waste cooking oils for valuable compounds production ...
Lopes, Marlene; Miranda, Sílvia M.; Belo, IsabelWaste cooking oils (WCO) are vegetable oils discarded after food frying and great amounts are produced worldwide. Its management is a challenge, due to the environmental risk of illegally disposal into rivers and landfills. The main approaches for WCO valorization included their incorporation as component of animal feed and biodiesel manufacturing. Yet, the development of new feasible approaches is attractive f...
Dissolved oxygen effect on microbial lipids production by Yarrowia lipolytica f...
Miranda, Sílvia M.; Pereira, Ana Sofia; Lopes, Marlene; Belo, IsabelVolatile fatty acids (VFAs) can be obtained from organic wastes through acidogenic fermentation and the yeast Yarrowia lipolytica can use those VFAs for the biosynthesis of lipids. Oxygen is an important factor of the bioconversion process of VFAs into microbial lipids by Y. lipolytica, thus the effect of oxygenation was evaluated in batch cultures of the yeast for two different strains, varying the stirring an...
Waste cooking oils as feedstock for lipase and lipid-rich biomass production
Lopes, Marlene; Miranda, Sílvia M.; Alves, Joana M.; Pereira, Ana Sofia; Belo, IsabelApproximately 1 million tons of waste cooking oils (WCO) are generated in European Union per year. Oftentimes, these oily wastes are discharged through public sewerage systems, increasing the water treatment costs in wastewater treatment plants. The oleaginous yeast Yarrowia lipolytica W29 was used to simultaneously degrade WCO and to produce lipase (market demand is increasing due to its application in the fie...
Selection of yeast species for hydrocarbons and phenolic compounds degradation
Lopes, Marlene; Ramôa, Renata; Miranda, Sílvia M.; Belo, Isabel[Excerpt] 1. Introduction. Large amounts of pollutant compounds are present in industrial effluents and, often, they are not totally degraded by physical and/or chemical methods before the discharge into the environment. Hydrocarbons and phenolic compounds are two examples of pollutants present in agro-industrial effluents, respectively in petroleum refinery effluents and olive mill wastewater (OMW). Biodegrada...
Simultaneous degradation of hydrocarbons and production of valuable compounds b...
Lopes, Marlene; Rama, Renata; Miranda, Sílvia M.; Belo, IsabelHydrocarbons are dangerous pollutants and great amounts of these compounds are released to the environment, due to the inadequate handling in the petroleum extraction or in the effluents of industries that use petroleum or its derivatives. The ability of Yarrowia lipolytica to efficiently degrade hydrocarbons (herein demonstrated with hexadecane and hexadecene) and use them as carbon source to grow and produce ...
Waste cooking oils: Low-cost substrate for co-production of lipase and microbia...
Miranda, Sílvia M.; Pereira, Ana Sofia; Belo, Isabel; Lopes, MarleneWaste Cooking Oils (WCO), generated from vegetable oils used at high temperatures in food frying, represent a highly dangerous threat to the environment. The ability of Y. lipolytica to grow and simultaneously produce lipase and accumulate lipids on this cheap and renewable carbon source was evaluated. A statistical experimental design based on Taguchi method was employed to assess the individual and combined e...
Bioconversion of volatile fatty acids into microbial lipids by Yarrowia lipolytica
Pereira, Ana Sofia; Miranda, Sílvia M.; Lopes, Marlene; Belo, IsabelVolatile fatty acids (VFAs) are short chain fatty acids that can be obtained from organic wastes through acidogenic fermentation and can be used as carbon source for microbial lipids production. The bioconversion of acetate, propionate and butyrate into microbial lipids by Yarrowia lipolytica W29 was evaluated, and the yeast shown the ability to grow using VFA as carbon source and accumulate lipids intracellula...