22 documents found, page 1 of 3
Digestive and metabolic consequences of on-growing greater amberjack (Seriola d...
Navarro-Guillén, C.; Perera, E.; Pérez-Hilario, D.; Martos-Sitcha, J.A.; Molina-Roque, L.; Gregorio, Silvia; Fonseca, Filomena; Fuentes, J.; Yúfera, M.Aiming to elucidate the effects of temperature on different aspects of the fish digestive physiology that may affect the growth, we examined, firstly, variations in growth performance, feed intake, digestive enzyme activities, expression of transport related genes, metabolites in plasma and liver, and oxidative stress response in juveniles of greater amberjack (Seriola dumerili) growing during two months at 18,...
High plant protein diet impairs growth performance and intestinal integrity in ...
Fuentes, Juan; Fonseca, Filomena; Gregorio, Silvia; Kussaba Miguel, Larissa; Perera, Erick; Alarcón-López, Francisco J.; Martos-Sitcha, Juan A.The Mediterranean aquaculture industry that produces mainly seabream and seabass is exploring alternative plant-based protein sources for fish feeds together with the diversification of fish species. Effective plant-based formulations require diverse sources and additives to maintain fish welfare and growth, which is especially important for carnivorous species. In this scenario, the objective of the present st...
From invasion to fish fodder: inclusion of the brown algae rugulopteryx okamura...
Fonseca, Filomena; Fuentes, Juan; Vizcaíno, Antonio Jesús; Alarcón, Francisco Javier; Mancera, Juan Miguel; Martínez-Rodríguez, GonzaloIn keeping with the premises of Blue Circular Economy in the European Union, the present study explored the possibility of using the invasive brown alga Rugulopteryx okamurae in aquafeeds for European sea bass (Dicen-trarchus labrax). Assuming the raw algae biomass could negatively impact animal performance, four experi-mental formulations were prepared, by including macroalgae material at 5%, using crude (CR),...
Editorial: microbiome dynamics as biomarkers of welfare status in aquatic species
Fonseca, Filomena; Fuentes, JuanThe importance of the microbiome in aquaculture species is widely acknowledged. The fish gut microbiota is essential for host fitness, modulated by diet and environment, and affects animal physiology. The gut microbiome optimizes nutrient uptake and immunomodulation and enhances host resilience against infectious diseases. Understanding microbiome dynamics in aquaculture species is vital in promoting animal hea...
First report of curvularia trifolii causing curvularia blight in agrostis stolo...
Coelho, Luísa; Borrero, C.; Bueno-Pallero, Francisco Angel; Guerrero, Carlos; Fonseca, Filomena; Reis, Mário; Avilés, M.; Dionísio, LídiaAlgarve region (Portugal) has nearly 40 golf courses with a significant economic impact. Summer surveys on 10 golf courses detected an unknown disease on one course in 2009 and on another course in 2012 and 2013 at 29 to 30°C daily average. The second course had symptoms on about 25% of the turf of two putting greens. Diseased bentgrass (Agrostis stolonifera L.) had a green dappled pattern with irregular patche...
Impact of ocean acidification on the intestinal microbiota of the marine sea br...
Fonseca, Filomena; Cerqueira, Ricardo; Fuentes, JuanWithin a scenario of increasing atmospheric CO2 and ocean acidification (OA), it is highly relevant to investigate its impacts not only on fish performance but also on fish intestinal microbiome and how that reflects on host performance and health. The main objective of this study was to establish if the intestinal microbiota of the sea bream (Sparus aurata) was affected by high level of CO2 in line with the pr...
First molecular characterization of grapevine virus B (GVB) in portuguese grape...
Fonseca, Filomena; Fernandes Duarte, Vilma Vanessa; Santos, Margarida Teixeira; Brazão, João; Eiras-Dias, EduardoThis work describes the first molecular characterization of grapevine virus B (GVB) in Portuguese grapevine cultivars. During a routine screening of 44 accessions in the National Collection of Grapevine Varieties (CAN PRT051), 17 were found infected with GVB in DAS-ELISA assays with commercial antibodies. However, only six of the corresponding isolates were successfully amplified using primer pairs described in...
Genetic variants of Grapevine leafroll-associated virus 2 infecting Portuguese ...
Fonseca, Filomena; Esteves, Filipa; Teixeira Santos, Margarida; Brazão, João; Eiras-Dias, José EduardoGenetic variability of 19 isolates of Grapevine leafroll-associated virus 2 (GLRaV-2) from Portuguese grapevine cultivars was characterized by sequencing the entire capsid protein (CP) gene of the virus. Global phylogenetic analysis of the CP gene, which included nucleotide sequences obtained in this study and complete homologous sequences from GenBank, showed segregation of GLRaV-2 variants from Portuguese iso...
Molecular data mining to improve antibody-based detection of grapevine leafroll...
Esteves, Filipa; Teixeira Santos, Margarida; Eiras-Dias, José Eduardo; Fonseca, FilomenaTesting for Grapevine leafroll-associated virus 1 (GLRaV-1) is mandatory in certification schemes of propagation material within the EU. Accurate and reliable diagnostic assays are necessary for implementation of this measure. During routine detection of GLRaV-1, using double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) and reverse transcription (RT) followed by polymerase chain reaction (PCR...
Development of an asymmetric PCR-ELISA typing method for citrus tristeza virus ...
Nolasco, Gustavo; Santos, C.; Silva, Goncalo; Fonseca, FilomenaThe coat protein gene of isolates of citrus tristeza virus (CTV) from 20 citrus-producing regions around the world was amplified by RT-PCR, TA cloned, and characterized by SSCP. Haplotypes that produced different patterns within each geographic region were sequenced and a database of 153 accessions of CTV was assembled. Phylogenetic analysis revealed the existence of seven well-defined clusters (Coefficient of ...