5 documents found, page 1 of 1
Effect of glucose oxidase incorporation in chitosan edible films properties
Lopes, M. I.; Martins, J. T.; Fonseca, L. P.; Vicente, A. A.Demand for biodegradable and environmentally friendly packaging has draw attention into the development of biodegradable, polymer based edible packaging films. Edible films’ functionality can be enhanced by incorporating active substances such as biocatalysts. The main objective of this work was to evaluate the effect of GOx incorporation in the mechanical and barrier properties of chitosan edible films. Films ...
Prediction of dynamic plasmid production by recombinant escherichia coli fed-ba...
Silva, T.; Lima, P.; Roxo-Rosa, M.; Hageman, S.; Fonseca, L. P.; Calado, C. R. C.A generalized regression neural network with external feedback was used to predict plasmid production in a fed-batch cultivation of recombinant Escherichia coli. The neu ral network was built out of the experimental data obtained on a few cultivations, of which the general strategy was based on an initial batch phase followed by an exponen tial feeding phase. The different cultivation conditions used resulted i...
Effect of tween-80 on stability and secretion of hydrophobic tagged-cutinases
Calado, Cecília; Brandão, M.; Biscaia, J.; Cabral, J. M. S.; Fonseca, L. P.To significantly enhance the downstream processing, two cutinase variants were constructed by genetic fusion small hydrophobic peptides (WP)2 and (WP)4, respectively. However, the fusion of these peptides impairs cutinase secretion by the host cell Saccharomyces cerevisiae and increases cutinase inactivation in the culture broth due to cutinase aggregation, resulting in cutinase activities per biomass of 56 % o...
Prediction of dynamic plasmid production by recombinant escherichia coli fed-ba...
Silva, T.; Lima, P.; Roxo-Rosa, M.; Hageman, S.; Fonseca, L. P.; Calado, CecíliaA generalized regression neural network with external feedback was used to predict plasmid production in a fed-batch cultivation of recombinant Escherichia coli. The neural network was built out of the experimental data obtained on a few cultivations, of which the general strategy was based on an initial batch phase followed by an exponential feeding phase. The different cultivation conditions used resulted in ...
Prediction of retention time of cutinases tagged with hydrophobic peptides in h...
Lienqueo, M. E.; Salazar, O.; Henriquez, K.; Calado, Cecília; Fonseca, L. P.; Cabral, J. M. S.Hydrophobic interaction chromatography (HIC) is an important technique for protein purification, which exploits the separation of proteins based on hydrophobic interactions between the stationary phase ligands and hydrophobic regions on the protein surface. One way of enhancing the purification efficiency by HIC is the addition of short sequences of peptide tags to the target protein by genetic engineering, whi...