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Effect of the Combined Treatments with LC2017 and Trichoderma atroviride Strain...

Reis, Pedro; Mondello, Vincenzo; Diniz, Inês; Alves, Artur; Rego, Cecília; Fontaine, Florence

Grapevine trunk diseases constitute one of the major problems for viticulture worldwide, with Botryosphaeria dieback considered as one of the most important of these diseases. In this work, we aimed to (i) evaluate the effect of the combination of two products, Esquive® (a Trichoderma-based product) and LC2017 (a low-copper-based product), in the control of Lasiodiplodia theobromae, by evaluating the internal l...


Combining an HA + CU (II) site-targeted copper-based product with a pruning wou...

Reis, Pedro; Gaspar, Ana; Alves, Artur; Fontaine, Florence; Rego, Cecília

The genus Lasiodiplodia has been reported from several grape growing regions and is considered as one of the fastest wood colonizers, causing Botryosphaeria dieback. The aim of this study was to (i) evaluate the efficacy of Esquive®, a biocontrol agent, on vineyard pruning wound protection, applied single or, in a combined protection strategy with a new site-targeted copper-based treatment (LC2017), and (ii) co...


Early season symptoms of stem, inflorescences and flowers of grapevine associat...

Reis, Pedro; Gaspar, Ana; Alves, Artur; Fontaine, Florence; Lourenço, Inês; Saramago, José; Mota, Mariana; Rego, Cecília

Botryosphaeria dieback caused by several Botryosphaeriaceae species is one of the most important grapevine trunk diseases a ecting vineyards worldwide. These fungi cause wedge-shaped perennial cankers and black streaking of the wood and have also been associated with intervein leaf chlorosis, dried or mummified berries, and eventually, the death of the plant. Early season symptoms may sometimes be disregarded b...


Understand the Potential Role of Aureobasidium pullulans, a Resident Microorgan...

Pinto, Cátia; Custódio, Valéria; Nunes, Mariana; Songy, Aurélie; Rabenoelina, Fanja; Courteaux, Barbara; Clément, Christophe; Gomes, Ana Catarina

Grapevine trunk diseases (GTDs) are one of the major concern amongst grapevine diseases, responsible for the decline of vineyards and for several economical losses. Since grapevine is naturally colonized by resident microorganisms such as Aureobasidium pullulans, the present challenge is to understand their biocontrol potential and how such microorganisms can be successfully integrated in the control of GTDs. I...


Draft genome sequence of Bacillus amyloliquefaciens subsp. plantarum strain Fit...

Pinto, Cátia; Sousa, Susana; Froufe, Hugo; Egas, Conceição; Clément, Christophe; Fontaine, Florence; Gomes, Ana C.

Bacillus amyloliquefaciens subsp. plantarum strain Fito_F321 is a naturally occurring strain in vineyard, with the ability to colonise grapevine and which unveils a naturally antagonistic potential against phytopathogens of grapevine, including those responsible for the Botryosphaeria dieback, a GTD disease. Herein we report the draft genome sequence of B. amyloliquefaciens subsp. plantarum Fito_F321, isolated ...


Phytotoxic metabolites from Neofusicoccum parvum, a pathogen of Botryosphaeria ...

Abou-Mansour, Eliane; Débieux, Jean-Luc; Ramírez-Suero, Montserrat; Bénard-Gellon, Mélanie; Magnin-Robert, Maryline; Spagnolo, Alessandro; Chong, Julie

Liquid chromatography-diode array screening of the organic extract of the cultures of 13 isolates of the fungus Neofusicoccum parvum, the main causal agent of botryosphaeria dieback of grapevine, showed similar metabolites. One strain was selected for further chemical studies and led to the isolation and characterisation of 13 metabolites. Structures were elucidated through spectroscopic analyses, including one...


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