8 documents found, page 1 of 1
DNA methylation patterns and karyotype analysis of off-type and normal phenotyp...
Cristofolini, Caroline; Vieira, Leila do Nascimento; Fraga, Hugo Pacheco de Freitas; Costa, Itayguara Ribeiro da; Guerra, Miguel Pedro; Pescador, RoseteAcca sellowiana is a native species to the southern region of Brazil, with secondary dispersion in Uruguay northeast. The potentially economic interest of this species motivated many fundamental and applied researches, such as micropropagation protocols development, including somatic embryogenesis (SE). Previous studies have reported the high frequency of off-type somatic embryos in A. sellowiana SE and low con...
Phylogenetic inference and SSR characterization of tropical woody bamboos tribe...
Rogalski, Marcelo; Vieira, Leila do Nascimento; Anjo, Karina Goulart dos; Faoro, Helisson; Fraga, Hugo Pacheco de Freitas; Greco, Thiago MachadoThe complete plastome sequencing is an efficient option for increasing phylogenetic resolution and evolutionary studies, as well as may greatly facilitate the use of plastid DNA markers in plant population genetic studies. Merostachys and Guadua stand out as the most common and the highest potential utilization bamboos indigenous of Brazil. Here, we sequenced the complete plastome sequences of the Brazilian Gua...
Effects of glutathione supplementation and carbon source during somatic embryog...
Pavei, Ana Flávia; Fraga, Hugo Pacheco de Freitas; Vieira, Leila do Nascimento; Guerra, Miguel PedroThe present study intended to investigate the effects of different glutathione (GSH) levels (0, 0.1, 0.5 and 1 mM) on the somatic embryogenesis (SE) induction of Acca sellowiana. Besides, we evaluated the effect of different carbon sources (sucrose and maltose) on the somatic embryos conversion. GSH-supplemented treatments resulted in improved SE induction rates (~70%) as compared to the control GSH-free (~35%...
The complete chloroplast genome sequence of Podocarpus lambertii: genome struct...
Vieira, Leila do Nascimento; Faoro, Helisson; Rogalski, Marcelo; Fraga, Hugo Pacheco de Freitas; Cardoso, Rodrigo Luis Alves; Souza, Emanuel Maltempi deNúmero de páginas não informado.; Podocarpus lambertii (Podocarpaceae) is a native conifer from the Brazilian Atlantic Forest Biome, which is considered one of the 25 biodiversity hotspots in the world. The advancement of next-generation sequencing technologies has enabled the rapid acquisition of whole chloroplast (cp) genome sequences at low cost. Several studies have proven the potential of cp genomes as too...
An improved protocol for intact chloroplasts and cpDNA isolation in conifers
Vieira, Leila do Nascimento; Faoro, Helisson; Fraga, Hugo Pacheco de Freitas; Rogalski, Marcelo; Souza, Emanuel Maltempi de; Pedrosa, Fábio de OliveiraPerforming chloroplast DNA (cpDNA) isolation is considered a major challenge among different plant groups,especially conifers. Isolating chloroplasts in conifers by such conventional methods as sucrose gradient and high salt has not been successful. So far, plastid genome sequencing protocols for conifer species have been based mainly on long-range PCR, which is known to be time-consuming and difficult to imple...
Dynamics in global DNA methylation and endogenous polyamine levels during proto...
Almeida, Vivian; Fraga, Hugo Pacheco de Freitas; Navarro, Bruno Bachiega; Guerra, Miguel Pedro; Pescador, RoseteCattleya tigrina is endemic to the Atlantic forest biome and classified as vulnerable in the Red Book of Brazilian Flora. In vitro techniques comprise valuable tools for the conservation of endangered plant species. The aim of the present study was to evaluate the morphological features, global DNA methylation levels and free polyamines during protocorm-like bodies (PLBs) induction of C. tigrina. Along with tha...
Biotecnologias para o uso e conservação de Araucaria angustifolia (Bertol.) Kun...
Fraga, Hugo Pacheco de FreitasTese (doutorado) - Universidade Federal de Santa Catarina, Centro de Ciências Agrárias, Programa de Pós-Graduação em Recursos Genéticos Vegetais, Florianópolis, 2015.; A conífera brasileira Araucaria angustifolia encontra-se dispersa no bioma Mata Atlântica e é conhecida popularmente como araucária ou pinheiro-brasileiro. Segundo a IUCN, a A. angustifolia encontra-se em perigo crítico de extinção, reforçando a ...
Efeitos da 5-azacitidina na embriogênese somática de Acca sellowiana (O. Berg.)...
Fraga, Hugo Pacheco de FreitasDissertação (mestrado) - Universidade Federal de Santa Catarina, Centro de Ciências Agrárias. Programa de Pós-Graduação em Recursos Genéticos Vegetais.; O presente trabalho teve como objetivo estudar o efeito do inibidor da metilação do DNA 5-Azacitidina (AzaC) na embriogênese somática em Acca sellowiana por meio de análises morfológicas e microscopia óptica, além de estudar os padrões de metilação do DNA globa...