5 documents found, page 1 of 1
Sequential Implantation of Micra and TriClip for Massive Tricuspid Regurgitatio...
Moreno, Nuno; Melica, Bruno; Puga, Luis; Amorim, Mario Jorge; Adão, LuisSevere tricuspid valve regurgitation (TR) is associated with high cardiovascular mortality. Safe and feasible interventional approaches to treat TR are of clinical relevance. Tricuspid transcatheter edge-to-edge repair (TEER) is a promising therapy for severe tricuspid regurgitation. Herein, we report an 81-year-old woman, with a history of double valve replacement, with recurrent symptomatic massive tricuspid ...
Severe Streptococcus suis Meningitis: A case report
Gonçalves, Joana; Rodrigues, Romina; Moreno, Nuno; Castro, Alexandra; Sá, Ana João; Lopes, Zélia; Ferraz, Graça; Lafuente, EstevãoStreptococcus suis (S. suis) is a commensal and opportunistic pathogen in pigs. Human infection is rare, occurring due to occupational exposure or consumption of pork products. The authors report an uncommon case of severe bacterial S. suis meningitis. A 42-year-old alcoholic male was admitted complaining of headache, fever, chills, vomiting and photophobia. One week prior to hospital admission the patient part...
Analysis of Protein Turnover by Quantitative SNAP-Based Pulse-Chase Imaging
Bodor, Dani L.; Rodríguez, Mariluz Gómez; Moreno, Nuno; Jansen, Lars E.T.Assessment of protein dynamics in living cells is crucial for understanding their biological properties and functions. The SNAP-tag, a self labeling suicide enzyme, presents a tool with unique features that can be adopted for determining protein dynamics in living cells. Here we present detailed protocols for the use of SNAP in fluorescent pulse-chase and quench-chase-pulse experiments. These time-slicing metho...
Comparative transcriptomics of Arabidopsis sperm cells
Borges, Filipe Filipe; Gomes, Gabriela; Gardner, Rui; Moreno, Nuno; McCormick, Sheila; Feijo, Jose A. Jose A.; Becker, Joeg D Joeg Dn flowering plants, the two sperm cells are embedded within the cytoplasm of the growing pollen tube and as such are passively transported to the embryo sac, wherein double fertilization occurs upon their release. Understanding the mechanisms and conditions by which male gametes mature and take part in fertilization are crucial goals in the study of plant reproduction. Studies of gene expression in male gametes...
Effect of fungal colonization on mechanical performance of cork
Silva Pereira, Cristina,; Soares, Giselle A. M.; Oliveira, Ana C.; Rosa, Maria Emılia; Pereira, Helena; Moreno, Nuno; San Romao, Maria VitoriaThe industrialization of traditional processes relies on the scientific ability to understand the empirical evidence associated with traditional knowledge. Cork manufacturing includes one operation known as stabilization, where humid cork slabs are extensively colonized by fungi. The implications of fungal growth on the chemical quality of cork through the analysis of putative fungal metabolites have already be...