7 documents found, page 1 of 1
Efficacy of a gE-deleted, bovine herpesvirus 1 (BoHV-1) inactivated vaccine
Silva,Alessandra D.; Esteves,Paulo A.; Dezen,Diogenes; Oliveira,Anna P.; Spilki,Fernando R.; Campos,Fabrício S.; Franco,Ana C.; Roehe,Paulo M.Bovine herpesvirus type 1 (BoHV-1) is recognized as a major cause of economic losses in cattle. Vaccination has been widely applied to minimize losses induced by BoHV-1 infections. We have previously reported the development of a differential BoHV-1 vaccine, based on a recombinant glycoprotein E (gE)-deleted virus (265gE-). In present paper the efficacy of such recombinant was evaluated as an inactivated vaccin...
Soroprevalência de herpesvírus bovinos tipos 1 e/ou 5 no Estado do Rio Grande d...
Holz,Carine L.; Cibulski,Samuel P.; Teixeira,Thais F.; Batista,Helena B.C.R.; Campos,Fabrício S.; Silva,Juliana R.; Varela,Ana Paula M.; Cenci,AlexanderEste estudo objetivou estimar a prevalência de anticorpos contra os herpesvírus bovinos tipos 1 e 5 (BoHV-1 e BoHV-5) no Estado do Rio Grande do Sul (RS), Brasil, frente a diferentes cepas de BoHV-1 e BoHV-5. As amostras de soro utilizadas foram extraídas de uma amostragem mais ampla, desenhada para estimar a prevalência de brucelose bovina no Estado. Todos os soros foram coletados de vacas com idade igual ou s...
Caracterização antigênica e molecular de oito amostras do vírus da doença de Au...
Silva,Alessandra D'Ávila da; Sortica,Vinícius A.; Braga,Alexandre C.; Spilki,Fernando R.; Franco,Ana C.; Esteves,Paulo A.; Rijsewijk,FransA doença de Aujeszky ou pseudoraiva (DA), causada pelo vírus da pseudoraiva (PRV) é a maior preocupação na produção de suínos. No estado do Rio Grande do Sul, Brasil, a DA foi somente detectada em 1954, em bovino. Em 2003, ocorreram dois surtos de encefalite em granjas na região norte do estado, fronteira com o estado de Santa Catarina. O vírus da doença de Aujeszky (VDA) foi isolado a partir de animais coletad...
Field evaluation of safety during gestation and horizontal spread of a recombin...
Spilki,Fernando R.; Silva,Alessandra D.; Batista,Helena Beatriz C. Ruthner; Oliveira,Anna P.; Winkelmann,Evandro; Franco,Ana C.; Porciúncula,Jorge A.Bovine herpesvirus type 1 (BoHV-1) is recognized as a major cause of respiratory, reproductive disease and abortion in cattle. Vaccination is widely applied to minimize losses induced by BoHV-1 infections; however, vaccination of dams during pregnancy with modified live virus (MLV) vaccines has been occasionally associated to abortions. We have previously reported the development of a BoHV-1 recombinant virus, ...
In vitro characterization of gE negative bovine herpesvirus types 1.1 (BHV-1.1)...
Spilki,Fernando R.; Franco,Ana C.; Rijsewijk,Frans A. M.; Weiblen,Rudi; Flores,Eduardo F.; Roehe,Paulo M.This study aimed the in vitro growth characterization of a previously constructed Brazilian bovine herpesvirus 1.2a with a deletion in the glycoprotein E gene (BHV-1.2a gE-). The plaque sizes, penetration and growth kinetics of the Brazilian BHV-1.2a gE- were studied and compared with the parental virus, as well as with a BHV-1.1 gE- recombinant derived from an European BHV-1.1 strain. No statistical difference...
Bovine herpesvirus type 5 (BHV-5) in a calf with rabies
Spilki,Fernando R.; Franco,Ana C.; Teixeira,Marjorie B.; Esteves,Paulo A.; Schaefer,Rejane; Schmidt,Eduardo; Lemos,Ricardo A.; Roehe,Paulo M.The brain of an one year old male calf which died with signs of neurological disease was submitted to the laboratory for rabies diagnosis. Microscopical findings included moderate mielitis, mild meningoencephalitis with perivascular cell cuffing and Negri inclusion bodies in Purkinje cells of the cerebellum. Rabies virus infection was further confirmed by the direct fluorescent antibody test as well as by mouse...
Construction and characterization of a glycoprotein E deletion mutant of bovine...
Franco,Ana C.; Rijsewijk,Frans A.M.; Flores,Eduardo F.; Weiblen,Rudi; Roehe,Paulo M.This paper describes the construction and characterization of a Brazilian strain of bovine herpesvirus type 1.2a (BoHV-1.2a) with a deletion of the glycoprotein E (gE) gene. The deletion was introduced by co-transfection of a deletion fragment containing the 5´and 3´gE flanking regions and genomic DNA of wild type BoHV-1 into bovine cells. Isolation of gE deletion mutant was performed by immunoperoxidase staini...