21 documents found, page 1 of 3
Designing protein nano-construct in ionic liquid: a boost in efficacy of cytoch...
Thayallath, Sarath Kumar; Shet, Sachin M.; Bisht, Meena; Bharadwaj, Pranav; Pereira, Matheus M.; Franklin, Gregory; Nataraj, S. K.; Mondal, DibyenduHerein, we present a simple approach to fabricate protein nanoconstructs by complexing cytochrome C (Cyt C) with silk nanofibrils (SNF) and choline dihydrogen phosphate ionic liquid (IL). The peroxidase activity of the IL modified Cyt C nanoconstruct (Cyt C + SNF + IL) increased significantly (2.5 to 10-fold) over unmodified Cyt C and showed enhanced catalytic activity and stability under harsh conditions, prov...
Overexpression of polygalacturonase-inhibiting protein (PGIP) gene from Hyperic...
Hou, Weina; Singh, Rupesh Kumar; Zhao, Pan; Martins, Viviana; Aguilar, Emmanuel; Canto, Tomás; Tenllado, Francisco; Franklin, Gregory; Dias, AlbertoHypericum perforatum L is a remarkable source of high-value secondary metabolites with increasing applications in pharmaceutical industry. However, improvement in the production of secondary metabolites through genetic engineering is a demanding task, as H. perforatum is not amenable to Agrobacterium tumefaciens-mediated transformation. In this study, we identified a Polygalacturonase-inhibiting protein (PGIP) ...
PEGylated ofloxacin nanoparticles render strong antibacterial activity against ...
Marslin, Gregory; Revina, A. M.; Khandelwal, V. K. M.; Balakumar, K.; Singh, Caroline Jeya Sheeba Daniel Sunder; Franklin, GregoryThe rise of bacterial resistance against important drugs threatens their clinical utility. Fluoroquinones, one of the most important classes of contemporary antibiotics has also reported to suffer bacterial resistance. Since the general mechanism of bacterial resistance against fluoroquinone antibiotics (e.g. ofloxacin) consists of target mutations resulting in reduced membrane permeability and increased efflux...
Delivery as nanoparticles reduces imatinib mesylate-induced cardiotoxicity and ...
Marslin, Gregory; Revina, Ann Mary; Khandelwal, Vinoth Kumar Megraj; Balakumar, Krishnamoorthy; Prakash, Jose; Franklin, Gregory; Sheeba, Caroline J.Clinical effectiveness of imatinib mesylate in cancer treatment is compromised by its off-target cardiotoxicity. In the present study, we have developed physically stable imatinib mesylate-loaded poly(lactide-co-glycolide) nanoparticles (INPs) that could sustainably release the drug, and studied its efficacy by in vitro anticancer and in vivo cardiotoxicity assays. MTT (methylthiazolyldiphenyl-tetrazolium bromi...
Antimicrobial activity of cream incorporated with silver nanoparticles biosynth...
Marslin, Gregory; Selvakesavan, Rajendran K.; Franklin, Gregory; Sarmento, Bruno; Dias, Alberto Carlos PiresWe report on the antimicrobial activity of a cream formulation of silver nanoparticles (AgNPs), biosynthesized using Withania somnifera extract. Aqueous extracts of leaves promoted efficient green synthesis of AgNPs compared to fruits and root extracts of W. somnifera. Biosynthesized AgNPs were characterized for their size and shape by physical-chemical techniques such as UV-visible spectroscopy, laser Doppler ...
Delivery as nanoparticles reduces imatinib mesylate-induced cardiotoxicity and ...
Marslin, Gregory; Revina, Ann Mary; Khandelwal, Vinoth Kumar Megraj; Balakumar, Krishnamoorthy; Prakash, Jose; Franklin, Gregory; Sheeba, Caroline JeyaClinical effectiveness of imatinib mesylate in cancer treatment is compromised by its off-target cardiotoxicity. In the present study, we have developed physically stable imatinib mesylate-loaded poly(lactide-co-glycolide) nanoparticles (INPs) that could sustainably release the drug, and studied its efficacy by in vitro anticancer and in vivo cardiotoxicity assays. MTT (methylthiazolyldiphenyl-tetrazolium bromi...
Test anxiety levels of board exam going students in Tamil Nadu, India
Mary, Revina Ann; Marslin, Gregory; Franklin, Gregory; Sheeba, Caroline J.The latest report by the National Crime Records Bureau has positioned Tamil Nadu as the Indian state with highest suicide rate. At least in part, this is happening due to exam pressure among adolescents, emphasizing the imperative need to understand the pattern of anxiety and various factors contributing to it among students. The present study was conducted to analyze the level of state anxiety among board exam...
Agrobacterium-elicited Hypericum perforatum cell methanolic extract shows antib...
Singh, R. K.; Hou, Weina; Dias, Alberto Carlos Pires; Franklin, GregoryHypericum perforatum (HP), an important medicinal plant that possesses several pharmacological properties, remains recalcitrant to Agrobacterium tumefaciens (AT) mediated transformation. Upon co-cultivation, AT viability was reduced completely HP cells defense response [1]. Further, we reported that the antibacterial and antioxidant activities of HP cells were increased several fold after elicitation with AT th...
Signaling pathways influencing tumor microenvironment and their exploitation fo...
Sheeba, Caroline Jeya; Marslin, Gregory; Revina, Ann Mary; Franklin, GregoryIn the recent years, the "tumor microenvironment" has been receiving growing attention due to its involvement in neoplastic transformation, tumor growth, invasion, and protection of tumor cells from host immune response. All these events are facilitated by chemical signals produced by the tumor as well as the surrounding stromal cells. This review is divided into two main parts in which the first part discusses...
Test anxiety levels of board exam going students in Tamil Nadu, India
Mary, Revina Ann; Marslin, Gregory; Franklin, Gregory; Sheeba, Caroline JeyaThe latest report by the National Crime Records Bureau has positioned Tamil Nadu as the Indian state with highest suicide rate. At least in part, this is happening due to exam pressure among adolescents, emphasizing the imperative need to understand the pattern of anxiety and various factors contributing to it among students. The present study was conducted to analyze the level of state anxiety among board exam...