24 documents found, page 1 of 3
Superhydrophobic materials for oil/water separation based on supported ionic li...
Nunes, J. C. F.; Freire, M. G.; Tavares, A. P. M.; Neves, M. C.Materials comprising a water contact angle (WCA) above 150˚ and a roll-off angle below 10˚ are designated as superhydrophobic. These materials have high potential to several relevant applications, e.g., anti-bacterial and oil/water separation. Oil spill accidents and industrial oily wastewater have emerged as serious threats to the environment and public health. Membrane separation is a widely used, easy, and e...
Production and purification of non-viral vectors for gene therapy applications
Valente, A. I.; Castro, L. S.; Rios, I. D.; Pedro, A. Q.; Tavares, A. P. M.; Sousa, F.; Freire, M. G.Cancer is the second cause of death worldwide with relevant societal and economic burdens. Approximately 19.3 million new cases and 10 million cancer deaths were registered in 2020, being projected an increase in cancer incidence in the coming years.1 To counteract this trend and mitigate consequences, multiple innovations have been achieved in oncology, from which the manufacturing of biopharmaceuticals as eff...
Silica-based supported ionic liquid-like phase materials for the flow-through d...
Nunes, J. C. F.; Almeida, M. R.; Paiva, G. B. de; Pedrolli, D. B.; Santos-Ebinuma, V. C.; Neves, M. C.; Freire, M. G.; Tavares, A. P. M.L-asparaginase (ASNase) is an amidohydrolase enzyme widely distributed in nature, but microorganisms are their preferential source. Nevertheless, high enzyme purity is required by the pharmaceutical industry. Silica-based supported ionic liquid-like phase (SSILLP) materials with different alkyl chain lengths at the cation source and Cl− as the anion source were investigated to purify ASNase. Silica functionaliz...
Development of an integrated process for the production and purification of lac...
Magalhães, F. F.; Alves, Carolina; Pedro, A. Q.; Freire, M. G.; Tavares, A. P.Extractive fermentation is an integrated process that uses aqueous biphasic systems to combine the production and primary purification procedures into a single step. With this concept, the main goal of this work was to design a bioprocess that integrates laccase production and purification using a genetically engineered yeast (Pichia pastoris), creating a competitive and sustainable process. Successful expressi...
Supported Ionic Liquids materials to remove insecticides
Francisco, R.; Almeida, C.; Sousa, A. C. A.; Neves, M.C.; Freire, M. G.Neonicotinoids insecticides are generally used to control pests in agriculture and for veterinary applications.1,2 Due to their extensive use, these compounds can be found in various environmental compartments, such as water, soils, and biota, which can be a problem of concern due to their toxicity against non-target organisms.2 Given this, it is of utmost priority to develop innovative and effective strategies...
Enzymatic production of polydopamine in aqueous biphasic systems
Magalhães, F. F.; Pereira, A. F.; Tavares, A. P.; Freire, M. G.No abstract available.
Novel systems for the production of polydopamine by biocatalysis
Pereira, A. F.; Magalhães, F. F.; Freire, M. G.; Tavares, A. P.Based on the principles of green chemistry, enzymatic biocatalysis contributes to a more eco-friendly and sustainable industrial chemistry. Therefore, its wide use in industrial and biotechnological processes has increasingly become more evident. One promising application is the enzymatic polymerization of various compounds. Laccase, a multicopper oxidase with high catalytic efficiency and low substrate specifi...
Supported ionic liquid materials for L-asparaginase immobilization
Nunes, J. C. F.; Almeida, M. R.; Santos-Ebinuma, V. C.; Neves, M. C.; Freire, M. G.; Tavares, A. P. M.L-asparaginase (ASNase) (L-asparagine amidohydrolase EC 3.5.1.1) has been widely used as a therapeutic agent in the treatment of acute lymphoblastic leukemia (ALL) and in the food industry for the removal of toxic acrylamide (formed in foods cooked at high temperatures). Accordingly, ASNase is also used in biosensors for leukemia diagnosis. To improve the performance of ASNase and overcome the limitations of fr...
Pristine carbon nanotubes for an efficient L-asparaginase immobilization
Cristóvão, R. O.; Almeida, M. R.; Barros, M. A.; Nunes, J. C. F.; Boaventura, R. A.R.; Loureiro, J. M.; Faria, J. L.; Neves, M. C.; Freire, M. G.The enzyme L-asparaginase (ASNase) presents effective antineoplastic properties for acute lymphoblastic leukemia treatment, besides their potential use in the food sector to decrease acrylamide formation. Considering their applications, the improvement of these enzyme properties by efficient immobilization techniques is in high demand. Carbon nanotubes are promising enzyme immobilization supports since these ma...
Supported ionic liquids as efficient strategies to reduce environmental contami...
Francisco, R.; Neves, M. C.; Pereira, M. M.; Sousa, A. C. A.; Freire, M. G.Cytostatic drugs are one of the most used therapeutic options to treat cancer. Considering that cancer prevalence is increasing at a fast rate, the consumption of these drugs is expected to increase in the future. Upon administration, cytostatics are mainly excreted trough urine. This urinary excretion is responsible for the contamination of sewage, wastewater treatment plants (WWTPs) effluents, and ultimately ...