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Ionic-liquid-processed keratin-based biocomposite films with cellulose and chit...

Polesca, C; Passos, H; Nakasu, PYS; Coutinho, JAP; Freire, MG; Hallett, JP

Poultry is a widely consumed meat worldwide; however, its industrial processing generates a significant amount of feather waste. Since the major component of chicken feathers is keratin (90 wt%), this study focused on using acetate-based ionic liquids (ILs) to fully dissolve chicken feathers and recover keratin, using a sustainable and cost-effective approach, ultimately allowing waste valorisation. The recover...


Improved L-Asparaginase Properties and Reusability by Immobilization onto Funct...

Barros, RAM; Raquel O. Cristóvão; Carneiro, IG; Barros, MA; Pereira, MM; Carabineiro, SAC; Freire, MG; Joaquim Luís Faria; Santos Ebinuma, VC

Enzyme immobilization can offer a range of significant advantages, including reusability, and increased selectivity, stability, and activity. In this work, a central composite design (CCD) of experiments and response surface methodology (RSM) were used to study, for the first time, the L-asparaginase (ASNase) immobilization onto functionalized carbon xerogels (CXs). The best results were achieved using CXs obta...


Valorisation of red beet waste: one-step extraction and separation of betalains...

Rosa, ME; Ferreira, AM; Neves, CMSS; Almeida, MT; Barros, R; Cristovão, AC; Sousa, Ana Catarina; Reis, PM; Rebelo, LPN; Esperança, JMSS; Coutinho, JAP

Globally, up to 50% of root crops, fruits and vegetables produced is wasted. Beetroot stems and leaves fit into this scenario, with only a small fraction being used in cattle food. One way of approaching this problem is through their valorisation, by extracting and recovering valuable compounds present in this type of waste that could be used in other applications, while contributing towards a circular economy....


Unveiling the Influence of Carbon Nanotube Diameter and Surface Modification on...

Raquel O. Cristóvão; Barros, RAM; Pinho, JG; Teixeira, LS; Neves, MC; Freire, MG; Joaquim Luís Faria; Santos Ebinuma, VC; Tavares, APM; Claudia G Silva

L-asparaginase (ASNase, EC 3.5.1.1) is an amidohydrolase enzyme known for its anti-cancer properties, with an ever-increasing commercial value. Immobilization has been studied to improve the enzyme's efficiency, enabling its recovery and reuse, enhancing its stability and half-life time. In this work, the effect of pH, contact time and enzyme concentration during the ASNase physical adsorption onto pristine and...


Recent Strategies and Applications for l-Asparaginase Confinement

Nunes, JCF; Raquel O. Cristóvão; Freire, MG; Santos Ebinuma, VC; Joaquim Luís Faria; Claudia G Silva; Tavares, APM

l-asparaginase (ASNase, EC 3.5.1.1) is an aminohydrolase enzyme with important uses in the therapeutic/pharmaceutical and food industries. Its main applications are as an anticancer drug, mostly for acute lymphoblastic leukaemia (ALL) treatment, and in acrylamide reduction when starch-rich foods are cooked at temperatures above 100 degrees C. Its use as a biosensor for asparagine in both industries has also bee...



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