353 documents found, page 1 of 36
A new approach for extracellular RNA recovery from Rhodovulum sulfidophilum
Riscado, Micaela; Carapito, Rita; Maia, Cláudio J.; Pichon, Chantal; Freire, Mara G.; Sponchioni, Mattia; Sousa, FaniThe development of RNA-based drugs is highly pursued due to the possibility of creating viable and effective therapies. However, their translation to clinical practice strongly depends on efficient technologies to produce substantial levels of these biomolecules, with high purity and high quality. RNAs are commonly produced by chemical or enzymatic methods, displaying these limitations. In this sense, recombina...
One-step purification of L-asparaginase from cell extracts using carbon xerogels
Cristóvão, Raquel O.; Barros, Rita A. M.; Marramaque, Teresa P.; Aguiar, Gonçalo G.; Almeida, Mafalda R.; Carabineiro, Sónia A. C.L-asparaginase (ASNase, EC 3.5.1.1) is an enzyme with wide applications in the pharmaceutical sector and food processing industries. It is mainly used as a biotherapeutic for treating Acute Lymphoblastic Leukemia (ALL) and to reduce acrylamide formation in starchy compounds. Despite its relevance, current purification methods for microbial enzymes involve complex and expensive techniques. To overcome this drawb...
Laccase-mediated dopamine polymerization: a bioinspired and sustainable coating...
Magalhães, Flávia F.; Alfieri, Maria Laura; Romanyuk, Konstantin; Kholkin, Andrei; Freire, Mara G.; Napolitano, Alessandra; Tavares, Ana Paula M.Polydopamine (PDA) has emerged as a widely used coating for various materials due to its unique properties such as robustness, biocompatibility, and antioxidant and photothermal activities. PDA coatings are usually prepared through autoxidation of dopamine under alkaline conditions, but this approach is generally timeconsuming and requires high concentrations of the starting monomer. To overcome these drawbacks...
Effective separation of nucleic acids from complex bacterial lysates using thre...
Pereira, Ana F.; Pedro, Augusto Q.; Castro, Leonor S.; Quental, Maria J.; Tavares, Ana P. M.; Branco, Luís C.; Coutinho, João A. P.; Sousa, FaniNo abstract available.
Enhanced Antimalarial Activity of Extracts of Artemisia annua L. Achieved with ...
Ferreira, Ana M.; Sales, Isabela; Santos, Sónia A.O.; Santos, Tiago; Nogueira, Fátima; Mattedi, Silvana; Pinho, Simão; Coutinho, João A.P.Artemisinin, a drug used to treat malaria, can be chemically synthesized or extracted from Artemisia annua L. However, the extraction method for artemisinin from biomass needs to be more sustainable while maintaining or enhancing its bioactivity. This work investigates the use of aqueous solutions of salts and ionic liquids with hydrotropic properties as alternative solvents for artemisinin extraction from Arte...
Enhancing biomarker detection in human serum for lung cancer diagnosis: aqueous...
Rosa, Marguerita E.; Mendes, Maria S. M.; Belchior, Diana C. V.; Coutinho, João A. P.; Silva, Francisca A. e; Freire, Mara G.Analysing biomarkers in human serum could be used as an effective and less invasive approach for the diagnosis of lung cancer; however, biomarker detection reliability is highly limited due to matrix effects. Herein, aqueous biphasic systems (ABS) are studied as platforms for human serum pretreatment, allowing the simultaneous depletion of high abundant proteins and biomarker extraction. By using ABS varying th...
Human platelet lysate-derived nanofibrils as building blocks to produce free-st...
Monteiro, Cátia F.; Gomes, Maria C.; Bharmoria, Pankaj; Freire, Mara G.; Coutinho, João A. P.; Custódio, Catarina A.; Mano, João F.Amyloid-like fibrils are garnering keen interest in biotechnology as supramolecular nanofunctional units to be used as biomimetic platforms to control cell behavior. Recent insights into fibril functionality have highlighted their importance in tissue structure, mechanical properties, and improved cell adhesion, emphasizing the need for scalable and high-kinetics fibril synthesis. In this study, we present the ...
The use of thermoreversible ionic liquid-based aqueous biphasic systems as an i...
Silva, Luís C. V.; Sousa, Maria I.; Correia, Andreia; Freire, Mara G.; Silva, Francisca A. e; Pedro, Augusto Q.COVID-19 reinforced the potential of messenger RNA (mRNA) vaccines as effective platforms to control disease outbreaks, given their rapid development, safety and efficacy. Nevertheless, mRNA manufacturing can be optimized by combining production with initial clarification steps. This work aims to integrate mRNA production and initial clarification steps, using thermoreversible ionic liquid-based aqueous biphasi...
Supported ionic liquids to purify microbial L-Asparaginase
Almeida, Mafalda R.; Nunes, João C. F.; Pereira, Matheus M.; Bento, Heitor B. S.; Pedrolli, Danielle B.; Santos-Ebinuma, Valéria C.; Neves, Márcia C.L-Asparaginase (ASNase) is a versatile enzyme that converts L-asparagine into ammonia and aspartic acid. This enzyme has applications in the food industry and health sector. However, high purity ASNase is required, resulting in high production costs. Therefore, in this work, two supported ionic liquids (SILs), specifically silica modified with dimethylbutylpropylammonium chloride ([Si][N3114]Cl) or triethylprop...
Portable colorimetric sensors for L-asparagine detection
Nunes, João C. F.; Neves, Márcia C.; Freire, Mara G.; Tavares, Ana P. M.L-asparaginase (ASNase) is an amidohydrolase enzyme used in the pharmaceutical industry as an anticancer agent in lymphoproliferative disorders treatment. ASNase is also applied in biosensors for L-asparagine detection in blood serum samples of acute lymphoblastic leukemia (ALL) patients. Nevertheless, the high cost of non-portable and sensitive L-asparagine detection methods, mainly performed by chromatography...