27 documents found, page 1 of 3
Anti-Toxoplasma gondii and anti-Neospora caninum antibodies in sheep from Paran...
Romanelli,Paulo Roberto; Matos,Andressa Maria Rorato Nascimento de; Pinto-Ferreira,Fernanda; Caldart,Eloiza Teles; Carmo,João Lucas Moura doAbstract The aim of this study was to evaluate the seroprevalence and factors associated with the presence of anti-Toxoplasma gondii and anti-Neospora caninum antibodies in sheep from Paraná state. The detection of antibodies for T. gondii and N. caninum was performed by homemade and commercial indirect ELISA, respectively. Multiple logistic regression analysis was used to verify the factors associated with the...
Techniques for inactivating Toxoplasma gondii oocysts: a systematic review
Pinto-Ferreira,Fernanda; Paschoal,Aline Ticiani Pereira; Pasquali,Aline Kuhn Sbruzzi; Bernardes,Juliana Correa; Caldart,Eloiza TelesAbstract The oocyst, a resistant form of Toxoplasma gondii, plays an important role in the transmission of this protozoan. The objective of this review was to report the methods capable of inactivating oocysts through a systematic review of the literature carried out in the Scientific Electronic Library Online, Web of Science, Science Direct, PubMed and Scopus databases. The keywords searched were (((effects OR...
Identification, molecular characterization and factors associated with occurren...
Oliveira,Juliana Silva de; Martins,Felippe Danyel Cardoso; Ladeia,Winni Alves; Cortela,Isadora de Britto; Valadares,Manuela FerrazAbstract Cattle are an important source of zoonotic species of Cryptosporidium for humans. The aim of this study was to investigate the presence of Cryptosporidium, identify the species and determine the risk factors relating to environment, animals and management among dairy calves in eight Brazilian states. A total of 408 fecal samples from calves aged 1-60 days were analyzed. An epidemiological questionnaire...
Autochthonous canine visceral leishmaniasis cases occur in Paraná state since 2...
Dias,Renata Cristina Ferreira; Pasquali,Aline Kuhn Sbruzzi; Thomaz-Soccol,Vanete; Pozzolo,Eliane Maria; Chiyo,Luciana; Alban,Silvana MariaAbstract The aim of this study was to confirm the emergence of canine visceral leishmaniasis among dogs in Foz do Iguaçu. The disease was diagnosed through the isolation and molecular identification of Leishmania infantum. In the first sample collection stage (2012), three lymph node aspirates and 46 buffy coat samples were obtained mostly from the dogs that were seroreagents for leishmaniasis. In the second sa...
Toxoplasma gondii and Neospora caninum infections and factors associated in goa...
Romanelli,Paulo Roberto; Matos,Andressa Maria Rorato Nascimento de; Pinto-Ferreira,Fernanda; Caldart,Eloiza Teles; Oliveira,Juliana Silva deAbstract The aim of this study was to determine the occurrence of anti-Toxoplasma gondii and anti-Neospora caninum antibodies and their associated factors among goats in farms from the Paraná state, South Brazil. The serological analysis was performed by indirect ELISA on 629 goat serum samples collected from 32 farms distributed in five mesorregions from the state. Seropositivity was observed in 30.7% of the a...
Surveillance of Giardia and Cryptosporidium in sewage from an urban area in Brazil
Martins,Felippe Danyel Cardoso; Ladeia,Winni Alves; Toledo,Roberta dos Santos; Garcia,João Luis; Navarro,Italmar Teodorico; Freire,Roberta LemosAbstract Cryptosporidium and Giardia are protozoan parasites that cause diarrhea in humans and animals. Molecular characterization of these pathogens in sewage may provide insight on their occurrence and prevalence in Brazil. This study aimed to investigate the presence of Giardia and Cryptosporidium in raw and treated sewage from Londrina, Paraná, Brazil. Samples were collected every two weeks during a year. S...
Investigation and environmental analysis of samples from outbreak of toxoplasmo...
Pinto-Ferreira,Fernanda; Mitsuka-Breganó,Regina; Monica,Thais Cabral; Martins,Felippe Danyel Cardoso; Matos,Ricardo Luís Nascimento de; Mareze,MarcelleAbstract The objective of this study was to report an outbreak of human toxoplasmosis at a research institution in Londrina, Paraná, from December 2015 to February 2016. Blood samples from 26 symptomatic individuals were collected and the microparticle chemiluminescence immunoassay was performed to detect IgM, IgG and specific IgG avidity test in the official laboratory. A total of 20 people with symptoms and s...
An outbreak of caprine toxoplasmosis - investigation and case report
Ferreira Neto,José Mauricio; Ferreira,Fernanda Pinto; Miura,Ana Carolina; Almeida,Jonatas Campos de; Martins,Felippe Danyel Cardoso; Souza,Marielen deABSTRACT: The present study aimed to investigate an abortion outbreak in a dairy goat herd in the municipality of Arapoti, Parana, Brazil. At the beginning of the outbreak, blood samples were collected from 33 goats with clinical signs; later, of the whole goat herd, two cats and two dogs. Milk samples were collected from 78 lactating goats. Four environmental soil samples and four samples of feed residue from ...
Antibodies anti-trypanosomatides in domestic cats in Paraná: who is at highest ...
Matos,Andressa Maria Rorato Nascimento de; Caldart,Eloiza Teles; Ferreira,Fernanda Pinto; Monteiro,Keila Clarine; Souza,Marielen deAbstract The aim of this study were to detect antibodies anti-Leishmania spp. and anti-Trypanosoma cruzi in two different populations of domestic cats (Felis catus domesticus) from North Paraná referred for surgical castration and to determine which characteristics of the animals studied may be associated with seropositivity. Serum samples from 679 cats were analyzed using enzyme-linked immunosorbent assay (ELI...
The effect of water source and soil supplementation on parasite contamination i...
Ferreira,Fernanda Pinto; Caldart,Eloiza Teles; Freire,Roberta Lemos; Mitsuka-Breganó,Regina; Freitas,Felipe Machado de; Miura,Ana CarolinaAbstract The objective of this study was to determine factors associated with vegetable contamination with zoonotic protozoan. Samples of water, soil and vegetables were collected from July/2014 to May/2016, totaling 83 samples, 21 properties of Londrina region, Paraná, Brazil. DNA amplification of Toxoplasma gondii, Cryptosporidium spp. and Giardia intestinalis in the samples was conducted using polymerase cha...