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Raw meat-based diet for pets: a neglected source of human exposure to Salmonell...

Ribeiro-Almeida, M.; Mourao, J.; Magalhães, Mafalda; Freitas, A.R.; Novais, Carla; Peixe, Luísa; Antunes, Patrícia

Background: The pet industry is expanding worldwide, particularly raw meat -based diets (RMBDs). There are concerns regarding the safety of RMBDs, especially their potential to spread clinically relevant antibioticresistant bacteria or zoonotic pathogens. Aim: We aimed to investigate whether dog food, including RMBD, commercially available in Portugal can be a source of Salmonella and/or other Enterobacteriacea...


Pet food safety: emerging bacterial hazards and implications for public health

Antunes, Patrícia; Novais, Carla; Peixe, L.; Freitas, A.R.

Bacterial hazards in pet food, especially in raw diets, are a current public health issue to both pets and humans. The most substantial body of evidence and consequent risk stems from food-borne pathogens such as Salmonella and bacteria resistant to last-resort antibiotics (e.g. colistin, third generation of cephalosporins, linezolid). State-of-the-art methods, particularly whole-genome sequencing, have been fu...


Optimization of DNA Extraction Methods for Metagenomic Analysis in Surface Wate...

Matos, C.; Duarte, B.; Freitas, A.R.; Valente, M.; Tavares, C.; Rodrigues, J.; Peixe, Luísa; Novais, Carla; Antunes, Patrícia


Current insights into the effects of cationic biocides exposure on Enterococcus...

Pereira, A.P.; Antunes, Patrícia; Peixe, L.; Freitas, A.R.; Novais, Carla

<jats:p>Cationic biocides (CBs), such as quaternary ammonium compounds and biguanides, are critical for controlling the spread of bacterial pathogens like <jats:italic>Enterococcus</jats:italic> spp., a leading cause of multidrug-resistant healthcare-associated infections. The widespread use of CBs in recent decades has prompted concerns about the potential emergence of <jats:italic>Enterococcus</jats:italic> s...


High occurrence of multidrug-resistant Esche-richia colifrom Holstein-Friesian ...

Quinteira, S.; Dias, A.B.; Pinho, L.; Brito, N.V.; Campos, Carla; Freitas, A.R.; Miranda, C.

Escherichia coli is currently a leading pathogen for deaths associated with antimicrobial resistance (AMR). AMR related to food-producing animals is a public health risk requiring a One Health approach. Animals are major reservoirs of antibiotic resistant E. coli which can easily reach humans through the food chain, direct contact, or the environment. To analyze the AMR profiles of E. coli in fecal samples from...


Uncovering Seasonal Variations in Antimicrobial-Resistant Enterococcus Diversit...

Matos, C.; Magalhães, A.; Pereira, F.; Duarte, B.; Freitas, A.R.; Valente, M.; Tavares, C.; Rodrigues, J.; Peixe, L.; Antunes, Patrícia; Novais, C.


Enterococcus spp. from chicken meat collected 20 years apart overcome multiple ...

Rebelo, A.; Duarte, B.; Ferreira, C.; Mourão, J.; Ribeiro, S.; Freitas, A.R.; Coque, T.M.; Willems, R.; Corander, J.; Peixe, L.; Antunes, Patrícia


Enterococcus spp. are susceptible to Quaternary Ammonium Compounds used in disi...

Pereira, A.P.; Antunes, Patrícia; Willems, R.; Corander, J.; Peixe, L.; Freitas, A.R.; Novais, Carla


Evolution of chlorhexidine susceptibility and of the EfrEF operon among Enteroc...

Pereira, AP; Antunes, Patrícia; Willems, R; Corander, J; Coque, TM; Peixe, L.; Freitas, A.R.; Novais, Carla

ABSTRACTChlorhexidine (CHX) is widely used to control the spread of pathogens (e.g. human/animal clinical settings, ambulatory care, food industry). E. faecalis, a major nosocomial pathogen, is broadly distributed in diverse hosts and environments facilitating its exposure to CHX over the years. Nevertheless, CHX activity against E. faecalisis understudied. Our goal was to assess CHX activity and the variabilit...


Chicken meat associated Enterococcus spp from 20 years apart overcomes multiple...

Rebelo, A.; Duarte, B.; Ferreira, C.; Mourão, J.; Ribeiro, S.; Freitas, A.R.; Coque, T.M.; Willems, R.; Corander, J.; Peixe, L.; Antunes, Patrícia


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