8 documents found, page 1 of 1
Evaluation of passive immunity transfer against G6P[1] rotavirus in holstein ca...
Rocha, Thaís G. [UNESP]; Silva, Ketherson R. [UNESP]; Silva, Fernanda D.F.; Alfieri, Amauri A.; Silva, Daniela G. [UNESP]Made available in DSpace on 2020-12-12T02:21:42Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-01-01; The main strategy to prevent bovine rotavirus group A (RVA) diarrhea in calves is to vaccinate late-term dams aiming to enhance passive immunity transfer of specific immunoglobulins against the virus. This study aimed to evaluate influence of parity in titers of immunoglobulin G (IgG), IgG1 and IgM in s...
Histopathological, immunohistochemical, and molecular study of BHV-5 infection ...
Cagnini, Didier Q. [UNESP]; Cunha, Paulo H. J.; Pantoja, Jose C. F. [UNESP]; Badial, Peres R. [UNESP]; Oliveira-Filho, Jose Paes de [UNESP]Made available in DSpace on 2015-10-21T13:09:46Z (GMT). No. of bitstreams: 0 Previous issue date: 2015-04-01. Added 1 bitstream(s) on 2015-10-22T09:53:11Z : No. of bitstreams: 1 S0100-736X2015000400337.pdf: 409488 bytes, checksum: 55affbb4b281ddd22ce343505f842431 (MD5); Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP); Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq); A meningoe...
Diarréia em bezerros da raça Nelore criados extensivamente: estudo clínico e et...
Oliveira Filho, José Paes de [UNESP]; Silva, Daniel P.G.; Pacheco, Marcelo D.; Mascarini, Luciene M. [UNESP]; Ribeiro, Márcio Garcia [UNESP]Submitted by Guilherme Lemeszenski (guilherme@nead.unesp.br) on 2013-08-22T18:50:40Z No. of bitstreams: 1 S0100-736X2007001000006.pdf: 102168 bytes, checksum: 3f9922581a04315b255b6bd7171fade0 (MD5); Made available in DSpace on 2013-08-22T18:50:40Z (GMT). No. of bitstreams: 1 S0100-736X2007001000006.pdf: 102168 bytes, checksum: 3f9922581a04315b255b6bd7171fade0 (MD5) Previous issue date: 2007-10-01; Made availabl...
Reproductive failures in beef cattle breeding herds with emphasis for infectiou...
Junqueira, José R. C.; Alfieri, Amauri A.The reproductive efficiency in beef cattle breeding herds is one of the aspects of greater highlight for the financial success of the investment. In the present revision the main parameters used to evaluate the reproductive performance of cows in extensive management will be approached, as well as the more important infectious causes of the reproductive failures that affect the costs of the production.; Na pecu...
Reproductive performance evaluation of a beef cattle herd naturally infected wi...
Junqueira, José Ricardo C.; Freitas, Júlio C. de; Alfieri, Alice F.; Alfieri, Amauri A.The aim of this study was the evaluation of some reproductive rates of a beef cattle herd that were naturally infected by the bovine herpesvirus 1 (BoHV-1), bovine viral diarrhea virus (BVDV) and Leptospira hardjo. In a herd with 1331 females, we randomly selected 208 animals that were distributed in three groups: i) 60 virgin heifers; ii) 60 first suckling cows; iii) 88 cows with two or more sucklings. All gro...
Comparison of the serologic tests of Indirect Immunofluorescence, Rapid Conglut...
Marana, Elizabete Regina Marangoni; Alfieri, Amauri A.; Andrade, Gisele Maria de; Freire, Roberta Lemos; Garcia, João Luís; Vidotto, OdilonThe serological techniques Rapid Conglutination Test (RCT), Indirect ELISA (iELISA) and Indirect Immunofluorescent Assay (IFA), using the competition ELISA (cELISA) as gold test, were comparatively evaluated to detect antibodies against Anaplasma marginale. A total of 453 sera from vaccinated and non vaccinated cattle and, collected from enzootic stability and instability areas were tested. iELISA, IFA and TCR ...
Comparison of different protocols for the bovine viral diarrhea virus detection...
Pilz, Daniela; Alfieri, Alice F.; Alfieri, Amauri A.The RT-PCR technique was optimized and evaluated to detect the 5’ untranslated region of bovine viral diarrhea virus (BVDV) from clinical samples that consisted of blood serum and whole blood artificially contaminated with the NADL strain of BVDV. To optimization of technique, the following conditions were evaluated: i) two pairs of primers, 103 / 372 (WEINSTOCK et al., 2001) and 324 / 326 (VILCEK et al., 1994)...
Development and evaluation of an enzyme-linked immunosorbent assay for the sero...
Ferreira, Melissa C.; Médici, Kerlei C.; Alfieri, Alice F.; Alfieri, Amauri A.An indirect enzyme-linked immunosorbent assay (ELISA) using non-purified antigen was developed for serological diagnosis of the bovine herpesvirus 1 (BoHV-1) infection. Some blocking substances to prevent nonspecific reactions were evaluated in different concentrations and combinations. The best results were obtained using 5% skim milk powder as blocking solution and the serum/conjugated buffer added with 10% M...