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Defatted nannochloropsis oculata biomass: waste or resource?

Sousa, Sérgio; Machado, Manuela; Coscueta, Ezequiel; Ferreira, Andreia S.; Nunes, Cláudia; Coimbra, Manuel A.; Freitas, Ana C.; Carvalho, Ana P.

Microalgae are recognized as a valuable source of a panoply of compounds. In addition to the extensively investigated lipid fraction comprising polyunsaturated fatty acids (PUFA), microalgae biomass also encompasses other compounds with potentially relevant biological activities. This work innovatively repurposes the defatted biomass (DB) of Nannochloropsis oculata, highlighting its potential value beyond the l...


Combining high pressure and electric fields towards Nannochloropsis oculata eic...

Sousa, Sérgio; Carvalho, Ana P.; Pinto, Carlos A.; Amaral, Renata A.; Saraiva, Jorge A.; Pereira, Ricardo Nuno Correia; Vicente, A. A.; Freitas, Ana C.

Nannochloropsis oculata is naturally rich in eicosapentaenoic acid (EPA). To turn this microalga into an economically viable source for commercial applications, extraction efficiency must be achieved. Pursuing this goal, emerging technologies such as high hydrostatic pressure (HHP) and moderate electric fields (MEF) were tested, aiming to increase EPA accessibility and subsequent extraction yields. The innovati...


Combining high pressure and electric fields towards nannochloropsis oculata eic...

Sousa, Sérgio; Carvalho, Ana P.; Pinto, Carlos A.; Amaral, Renata A.; Saraiva, Jorge A.; Pereira, Ricardo N.; Vicente, António A.; Freitas, Ana C.

Nannochloropsis oculata is naturally rich in eicosapentaenoic acid (EPA). To turn this microalga into an economically viable source for commercial applications, extraction efficiency must be achieved. Pursuing this goal, emerging technologies such as high hydrostatic pressure (HHP) and moderate electric fields (MEF) were tested, aiming to increase EPA accessibility and subsequent extraction yields. The innovati...


Can growth of nannochloropsis oculata under modulated stress enhance its lipid-...

Sousa, Sérgio C.; Machado, Manuela; Freitas, Ana C.; Gomes, Ana M.; Carvalho, Ana P.

Nannochloropsis oculata is well-recognized as a potential microalgal source of valuable compounds such as polyunsaturated fatty acids, particularly, eicosapentaenoic acid (EPA). The content and profile of these lipids is highly dependent on the growth conditions and can, therefore, be tailored through modulation of the growth parameters, specifically, temperature. Moreover, biological activities are composition...


Antibacterial and antioxidant potential of aqueous extracts of bio-residues fro...

Sousa, Ana Sofia; Nunes, Catarina; Barros, Joana; Ferreira, Inês; Freitas, Ana C.; Morais, Alcina M. M. B.; Nunes, João; Pintado, Manuela

The production of fresh mushrooms results in a large quantity of bio-residues, which may account for more than 20% of a crop volume and contributes to production costs and environmental impact, if not used. These products consist mainly of stalks, mushrooms of irregular dimensions and shape and basal material. Thus, the main objective of MicoBioExtract project is the valorisation of these bio-residues and the d...


The role of cereals in the industry of plant-based foods

Barbosa, Joana C.; Rodrigues, Dina; Almeida, Diana; Machado, Daniela; Franco, Isabel; Inácio, Joana; Freitas, Ana C.; Gomes, Ana M.

Current trends in human feeding promote a more plant-based diet, for several reasons [1]. Ethical and environmental concerns are among the most common reasons why people chose to avoid animal-based products [2], [3]. Also, there is an increasing perception that the excessive consumption of animal-based products is associated with higher risk of developing chronic diseases [4]. Thus, the demand for innovative an...


Challenges in functional foods development based on bioactive lipids

Machado, Manuela; Freitas, Ana C.; Pintado, Manuela; Gomes, Ana Maria

Lipids have an important role in human health. Their health benefits include the reduction of cardiovascular diseases, the improvement of cognitive function, and they act as antiinflammatory and antioxidant compounds. Despite these benefits, the consumption of these compounds is very low, due to the actual lifestyles and lack of knowledge by the general consumer. On the other hand, during food production and st...


Modulated stress to balance Nannochloropsis oculata growth and eicosapentaenoic...

Sousa, Sérgio; Freitas, Ana C.; Gomes, Ana M.; Carvalho, Ana P.

Two environmental parameters, temperature and light intensity, were independently used as stress modulators to enhance eicosapentaenoic acid (EPA) production by the microalga Nannochloropsis oculata, without hindering biomass production. A sinusoidal approach was used, as environmental conditions were alternated between optimum and stress status in multi-day cycles. Low temperatures (5 and 10 °C) and light inte...


Pleurotus ostreatus bio-residues: a source of bioactives with antioxidant and a...

Sousa, Ana Sofia; Nunes, Catarina; Barros, Joana; Ferreira, Inês; Freitas, Ana C.; Morais, Alcina M. M. B.; Nunes, João; Pintado, Manuela

Mushrooms are known to be a rich source of functional and bioactive compounds. Among these, phenolic compounds and polysaccharides, mainly β-glucans, stand out due to their antioxidant and immunomodulatory effects, respectively. In the present work, the total phenolic compounds content, the content in β-glucans and bioactivity properties from the bio-residues obtained from mushroom production were evaluated in ...


Phenolics content and antioxidant activity of aqueous extracts from bio-residue...

Sousa, Ana Sofia; Marçal, Sara; Nunes, Catarina; Barros, Joana; Ferreira, Inês; Freitas, Ana C.; Morais, Alcina M. M. B.; Nunes, João; Pintado, Manuela

Mushrooms are known as a rich source of functional and bioactive compounds. Among these, phenolic compounds as important antioxidants are included. In the present work, the antioxidant potential and total phenolic compounds from bio-residues obtained from edible mushroom production were evaluated in order to develop a circular bioeconomy approach. The studied species was Agrocybe cylindraceae. The extraction wa...


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