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Labelling composition, methods and uses thereof

Aguiar, Tatiana Quinta; Oliveira, Juliana; Barros, Ana Rita; Bouça, Verónica; Ramada, David; Freitas, Ana Isabel; Domingues, Lucília

The present disclosure relates to a labelled article comprising a substrate selected from a list consisting of at least one of the following substrates: paper, cork, leather, yarns, threads, textiles, fabrics, a particle, or combinations thereof; a reversibly switchable fluorescent peptide; and a binder peptide, preferably more than one binder, wherein the binder binds the fluorescent peptide to the substrate, ...


Impact of genetic information on coronary disease risk in Madeira

Mendonça, Maria Isabel; Pereira, Andreia; Monteiro, Joel; Sousa, João Adriano; Santos, Marina; Temtem, Margarida; Borges, Sofia; Henriques, Eva

INTRODUCTION: Coronary artery disease (CAD), characterized by an atherogenic process in the coronary arteries, is one of the leading causes of death in Madeira. The GENEMACOR (GENEs in MAdeira and CORonary Disease) study sought to investigate the main risk factors - environmental and genetic - and estimate whether a genetic risk score (GRS) improves CAD prediction, discrimination and reclassification. METHODS: ...


Tag-mediated single-step purification and immobilization of recombinant protein...

Freitas, Ana Isabel; Domingues, Lucília; Aguiar, Tatiana Quinta

Background The potential applications of protein-engineered functional materials are so wide and exciting that the interest in these eco-friendly advanced materials will further expand in the future. Tag-mediated protein purification/immobilization technologies have emerged as green and cost-effective approaches for the fabrication of such materials. Strategies that combine the purification and immobilization o...


Sustainable identity through switchable fluorescence: smart textiles with rever...

Barros, Ana; Ramada, David; Bouça, Verónica; Oliveira, Juliana; Freitas, Ana Isabel; Domingues, Lucília; Aguiar, Tatiana Quinta

[Excerpt] Colour-changing materials with con - trollable properties obtained by low cost and biotechnological means are highly desirable for smart innovative products and applications such as anti-counterfeiting and security printing. Protein-based nanotechno - logy is an ever-growing research field due to the versatility and adap - tability of these biomolecule building blocks and the innovative functiona - li...


Solid-binding peptides as affinity tags for one-step purification and immobiliz...

Freitas, Ana Isabel; Domingues, Lucília; Aguiar, Tatiana Quinta

Solid-binding peptides (SBPs) are short peptides that show selectivity and bind with high affinity to the surfaces of specific solid materials, such as metals, metal-oxides, and polymers, via non-covalent interactions [1]. When attached to functional proteins, SBPs direct their immobilization onto solid supports without compromising their functionality. Therefore, SBPs have been fairly used in applications such...


Bare silica as an alternative matrix for affinity purification/immobilization o...

Freitas, Ana Isabel; Domingues, Lucília; Aguiar, Tatiana Quinta

Green protein purification/immobilization processes based on low-cost, earth-abundant, and eco-friendly affinity matrices are highly desirable. Unmodified silica matrices fit well these demands. Since histidine-rich silica-binding peptides are frequently isolated in biopanning experiments, this work aimed at assessing the viability of using bare silica as an alternative matrix for the purification/immobilizatio...


WITHDRAWN

Mendonça, Maria Isabel; Pereira, Andreia; Monteiro, Joel; Sousa, João Adriano; Santos, Marina; Temtem, Margarida; Borges, Sofia; Henriques, Eva

The Publisher regrets that this article is an accidental duplication of an article that has already been published, 10.1016/j.repc.2022.10.005. The duplicate article has therefore been withdrawn. The full Elsevier Policy on Article Withdrawal can be found at https://www.elsevier.com/about/our-business/policies/article-withdrawal.


Innovative eco-sustainable photocontrolable and reversibly photoswitchable fluo...

Oliveira, Juliana; Bouça, Verónica; Barros, Ana; Ramada, David; Freitas, Ana Isabel; Domingues, Lucília; Aguiar, Tatiana Quinta

Colour-changing materials with controllable properties are highly desirable for technical, smart innovative products. Current developments commonly incorporate photo/thermochromic inks based on molecules obtained by non-sustainable petroleum based sources that do not allow controlled light-driven switching between colors. To overcome these limitations, our project explores the development of new eco-sustainable...


Smart textile fabrication with photocontrolable, reversibly photoswitchable and...

Bouça, Verónica; Oliveira, Juliana; Barros, Ana; Ramada, David; Coelho, Lorena; Freitas, Ana Isabel; Romão, João; Domingues, Lucília

Color-changing materials with controllable photoswitching properties are highly desirable for technical and smart textile products. In the quest for innovative light-responsive textiles, many efforts have been made to develop materials that can be easily incorporated into fibers and/or fabrics by conventional dyeing, printing or coating processes. Current developments commonly incorporate photochromic inks that...


Low-cost and eco-friendly downstream processes for single-step purification and...

Aguiar, Tatiana Quinta; Freitas, Ana Isabel; Domingues, Lucília

Given the simplicity, versatility and high-efficiency of affinity tag-based separation and recovery systems, affinity tags have become indispensable tools for the purification of recombinant proteins produced by microbial hosts. Since downstream processing is the main contributor for the overall recombinant protein production costs, for applications such as enzyme immobilization and material functionalization, ...


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