34 documents found, page 1 of 4
Labelling composition, methods and uses thereof
Aguiar, Tatiana Quinta; Oliveira, Juliana; Barros, Ana Rita; Bouça, Verónica; Ramada, David; Freitas, Ana Isabel; Domingues, LucíliaThe present disclosure relates to a labelled article comprising a substrate selected from a list consisting of at least one of the following substrates: paper, cork, leather, yarns, threads, textiles, fabrics, a particle, or combinations thereof; a reversibly switchable fluorescent peptide; and a binder peptide, preferably more than one binder, wherein the binder binds the fluorescent peptide to the substrate, ...
Tag-mediated single-step purification and immobilization of recombinant protein...
Freitas, Ana Isabel; Domingues, Lucília; Aguiar, Tatiana QuintaBackground The potential applications of protein-engineered functional materials are so wide and exciting that the interest in these eco-friendly advanced materials will further expand in the future. Tag-mediated protein purification/immobilization technologies have emerged as green and cost-effective approaches for the fabrication of such materials. Strategies that combine the purification and immobilization o...
Sustainable identity through switchable fluorescence: smart textiles with rever...
Barros, Ana; Ramada, David; Bouça, Verónica; Oliveira, Juliana; Freitas, Ana Isabel; Domingues, Lucília; Aguiar, Tatiana Quinta[Excerpt] Colour-changing materials with con - trollable properties obtained by low cost and biotechnological means are highly desirable for smart innovative products and applications such as anti-counterfeiting and security printing. Protein-based nanotechno - logy is an ever-growing research field due to the versatility and adap - tability of these biomolecule building blocks and the innovative functiona - li...
Solid-binding peptides as affinity tags for one-step purification and immobiliz...
Freitas, Ana Isabel; Domingues, Lucília; Aguiar, Tatiana QuintaSolid-binding peptides (SBPs) are short peptides that show selectivity and bind with high affinity to the surfaces of specific solid materials, such as metals, metal-oxides, and polymers, via non-covalent interactions [1]. When attached to functional proteins, SBPs direct their immobilization onto solid supports without compromising their functionality. Therefore, SBPs have been fairly used in applications such...
Bare silica as an alternative matrix for affinity purification/immobilization o...
Freitas, Ana Isabel; Domingues, Lucília; Aguiar, Tatiana QuintaGreen protein purification/immobilization processes based on low-cost, earth-abundant, and eco-friendly affinity matrices are highly desirable. Unmodified silica matrices fit well these demands. Since histidine-rich silica-binding peptides are frequently isolated in biopanning experiments, this work aimed at assessing the viability of using bare silica as an alternative matrix for the purification/immobilizatio...
Cytotoxicity of frutalin on distinct cancer cells is independent of its glycosy...
Oliveira, Carla; Freitas, Ana Isabel; Campos, Nair; Saraiva, Lucília; Domingues, LucíliaFrutalin is a plant lectin with beneficial immunobiological action, although the access to its active form is still restricted. Moreover, there is a knowledge gap on isoform activity and glycosylation impact on its bioactivity, and recombinant production protocols were seen as ineffective. Here, a simpler and faster production and purification protocol was developed, attaining a yield of purified frutalin 3.3-f...
Innovative eco-sustainable photocontrolable and reversibly photoswitchable fluo...
Oliveira, Juliana; Bouça, Verónica; Barros, Ana; Ramada, David; Freitas, Ana Isabel; Domingues, Lucília; Aguiar, Tatiana QuintaColour-changing materials with controllable properties are highly desirable for technical, smart innovative products. Current developments commonly incorporate photo/thermochromic inks based on molecules obtained by non-sustainable petroleum based sources that do not allow controlled light-driven switching between colors. To overcome these limitations, our project explores the development of new eco-sustainable...
Smart textile fabrication with photocontrolable, reversibly photoswitchable and...
Bouça, Verónica; Oliveira, Juliana; Barros, Ana; Ramada, David; Coelho, Lorena; Freitas, Ana Isabel; Romão, João; Domingues, LucíliaColor-changing materials with controllable photoswitching properties are highly desirable for technical and smart textile products. In the quest for innovative light-responsive textiles, many efforts have been made to develop materials that can be easily incorporated into fibers and/or fabrics by conventional dyeing, printing or coating processes. Current developments commonly incorporate photochromic inks that...
Low-cost and eco-friendly downstream processes for single-step purification and...
Aguiar, Tatiana Quinta; Freitas, Ana Isabel; Domingues, LucíliaGiven the simplicity, versatility and high-efficiency of affinity tag-based separation and recovery systems, affinity tags have become indispensable tools for the purification of recombinant proteins produced by microbial hosts. Since downstream processing is the main contributor for the overall recombinant protein production costs, for applications such as enzyme immobilization and material functionalization, ...
Affinity purification/immobilization of His-tagged proteins using unmodified si...
Freitas, Ana Isabel; Domingues, Lucília; Aguiar, Tatiana QuintaThe development of affinity-based purification/immobilization schemes relying on low-cost, earth-abundant and environment-friendly matrices is highly desirable. Silica fits well this purpose. Protein purification/immobilization schemes using unmodified silica matrices have been developed based on silica-binding affinity peptides such as the Si-tag, CotB1/CotB1p and Car9. These positively charged peptides (rich ...