11 documents found, page 1 of 2

Sobredotação e comportamento disruptivo em contexto familiar

Este estudo integra o projeto de investigação, intitulado “Giftedness and the social and emotional well-being of the gifted” e tem como principal objetivo analisar a relação entre a sobredotação e o comportamento disruptivo em contexto familiar, comparando-se um grupo de crianças sobredotadas com um grupo de crianças não sobredotadas. Foi aplicado um inquérito por questionário online a 41 pais, que continha que...

Antimicrobial synergism against different lineages of methicillin-resistant Sta...

Aim To evaluate the synergistic activity of antimicrobial drugs against lineages of methicillin-resistant Staphylococcus aureus (MRSA) carrying SCCmec IV. The biofilm production and related genes were also detected. Methods and Results Forty two MRSA isolates were tested for biofilm production and related genes. Biofilm/biomass susceptibility to gentamicin (G), linezolid (L), rifampicin (R) and vancomycin (V) w...

Isolation of clinical strains of Staphylococcus epidermidis from a Portuguese h...

Staphylococcus epidermidis has been documented as an emergent pathogen responsible for many healthcareassociated infections (HAIs). These infections are an increasing cause of major concern not only due to the high distribution of methicillin resistance, but also due to their ability to form biofilm, which increases their persistence, impairs patient’s quality of life and leads to failed treatment and extra cos...

Medium supplementation with magnesium prevented the induction of dormancy in bi...

Staphylococcus epidermidis is a commensal bacterium that colonizes the skin and mucous membranes, being the most prevalent staphylococcal species found in humans. However, S. epidermidis has the ability of colonize indwelling medical devices establishing biofilms, which makes this bacteria a common cause of bacteraemia particularly in immunocompromised individuals and neonates [1][2]. The presence of high amoun...

Genotypic and phenotypic differences between nosocomial and commensal Staphyloc...

Staphylococcus epidermidis is a common causative agent of relapsing and persistent hospital-acquired infections, not only because this bacterium belongs to the patients’ microbiota(causing autoinfection) but also because it is easily transmitted by healthcare workers (causing cross-infections). The goal of the present study was to characterize, for the first time, the biofilm-forming ability and virulence assoc...

A first new look into the interaction of Staphylococcus epidermidis biofilm-rel...

The widespread application of indwelling medical devices in the clinical setting, together with the remarkable ability of the commensal Staphylococcus epidermidis to adhere to these surfaces and form biofilms, has given to this bacterium the recognition of being a leading causative agent of nosocomial infections. Biofilms lifecycle is currently divided into 4 main steps: initial adhesion, accumulation, maturati...

Staphylococcus epidermidis biofilm lifecycle and its virulence: from plantkonic...

Staphylococcus epidermidis biofilm dispersal cells : an intermediary phenotype?

Optimizing a qPCR gene expression quantification assay for S. epidermidis biofi...

Staphylococcus epidermidis biofilm-related infections are a current concern within the medical community due to their high incidence and prevalence, particularly in patients with indwelling medical devices. Biofilm gene expression analysis by quantitative real-time PCR (qPCR) has been increasingly used to understand the role of biofilm formation in the pathogenesis of S. epidermidis infections. However, dependi...

Optimization of a protocol for gene expression using biofilm cells from S. epid...

Gene expression assays are one of the most common tools used nowadays to evaluate the importance of genes in many different life sciences areas, namely, in clinical microbiology. Since most gene expression kits for qPCR have been optimized for assays with planktonic cells it is important to also optimize protocols for this type of assays, to be used with biofilms. Biofilms are communities of bacteria that grow ...