28 documents found, page 1 of 3

Bacterial load and dynamics: exploring their potential as biomarkers for postmo...

Background: Establishing the postmortem interval (PMI), the time since death, is vital in forensic investigations but remains challenging due to multiple factors like the cause of death, body location, and environmental conditions [1]. Traditional PMI estimation methods include Algor Mortis, Rigor Mortis, and Livor Mortis, along with complementary approaches from entomology, botany, and microbiology, though all...

Identification of linezolid-resistant Enterococcus spp. in calves: findings fro...

Background: Antimicrobial resistance (AMR) is a global health threat requiring a One Health approach, as antibiotic-resistant bacteria can spread between animals and humans. Enterococcus spp., particularly E. faecium (Efm) and E. faecalis (Efs), are key AMR indicators due to their role as gut commensals and their potential as reservoirs of resistance genes. They are also opportunistic pathogens that can cause s...

MicroMundo@IUCS_CESPU (2022-2024): uncovering soil’s hidden treasures and its i...

Background: MicroMundo@IUCS_CESPU (MMIC) is a service-learning pedagogical approach allowing university/school students to expand their knowledge on soil biodiversity, antimicrobial resistance (AMR) and experimental sciences [1]. School students experience a real research project by processing soil samples and looking for potential antibiotic-producing microorganisms [2,3]. Objective: To evaluate past MMIC edit...

Exploring antimicrobial resistance in autochthonous Portuguese hens: a study of...

Background: The widespread use of antibiotics in intensive animal farming significantly contributes to antimicrobial resistance (AMR), posing a serious global public health threat [1]. Despite this, little is known about the role of autochthonous Portuguese laying hens as reservoirs of antibiotic-resistant Escherichia coli. Raised in extensive farming systems with reduced antibiotic exposure, these indigenous b...

Diagnostic pitfalls in Staphylococcus spp. surveillance: lessons from a multina...

Background: Staphylococcus aureus is a leading cause of severe and hard-to-treat human infections, particularly when resistant to cefoxitin due to the presence of the mecA gene [1]. However, diagnostic challenges arise from the misidentification of S. aureus and related species when relying on classical identification methods (mannitol fermentation; coagulase production), as well as from the detection of strain...

Colonization and diversity of Klebsiella pneumoniae species complex (KpSC) in t...

Mapping of key bacterial species for Postmorteminterval calculation

Estimation of the PostmortemInterval (PMI), the time elapsed since death, is one of the most challenging issues in forensic sciences. Most studies focus on extensive bacterial sequencing, but culture-based experiments for higher taxonomic resolution remain scarce. We aimed to analyze total bacterial counts and map Enterococcus faecalis, Staphylococcus aureusand Escherichia coliin different organs and timepoints...

Dairy cattle and the iconic autochthonous cattle in northern Portugal are reser...

Animals destined for human consumption play a key role in potentially transmitting bacteria carrying antibiotic resistance genes. However, there is limited knowledge about the carriage of antibiotic-resistant bacteria in native breeds. We aimed to characterize the phenotypic profiles and antibiotic resistance genes in Escherichia coli isolated from bovines, including three native Portuguese bovine breeds. Forty...

Bacteriocin dynamics in Enterococcus faecium and Enterococcus lactis: implicati...

Background: Enterococcus faecium-Efm and E.lactis-Elts (former Efm-cladeB) colonize the human gut, with Efm also being a leading hospital-pathogen. Dynamics influencing strain dominance in competitive environments (e.g., infection/colonization) are not fully understood, but bacteriocins may provide competitive advantage to clinical Efm or commensal Elts strains. Objective: We explored bacteriocin content of con...

Colistin-resistant Escherichia coli in calves and adult cattle from Portuguese ...

Background: Antimicrobial resistance poses a pressing and significant challenge, driven by the intricate interplay among animals, humans, and the environment [1]. Escherichia coli, a commensal within animal and human gut microbiota, stands as one of the most ubiquitous bacteria. With a multitude of resistance genes, many E. coli strains exhibit a multidrug-resistant phenotype, diminishing the effectiveness...