40 documents found, page 1 of 4
Sustainable Production of Poly(3-hydroxybutyrate) Using Eucalyptus Bark: Integr...
Matias, João; Rodrigues, Thomas; Torres, Cristiana A. V.; Marques, Susana; Ribeiro, Belina; Gírio, Francisco; Reis, Maria A.; Freitas, FilomenaABSTRACT: This study integrates the valorization of a lignocellulose material into poly(3-hydroxybutyrate), P(3HB), with biopolymer extraction from bacterial cells with the enzyme alcalase. The work focused on Burkholderia thailandensis DSM 13276 as the P(3HB) producer and on eucalyptus bark, a byproduct from the pulp industry, as the sole feedstock for bacterial cultivation. The eucalyptus bark was hydrolyzed ...
Solid Microneedles from Poly(3-hydroxybutyrate-co-3-hydroxyvalerate-co-3-hydrox...
Araújo, Diana; Santos, Francisco; Igreja, Rui; Freitas, FilomenaBackground: Solid microneedles (MNs) are effective transdermal delivery devices but are commonly fabricated from metallic or non-biodegradable materials, raising concerns related to sustainability, waste management, and processing constraints. This study aimed to evaluate the suitability of the biodegradable biopolyester poly(3-hydroxybutyrate-co-3-hydroxyvalerate-co-3-hydroxyhexanoate) (PHBHVHHx) as a structur...
Biopolymers Derived from Forest Biomass for the Sustainable Textile Industry
Dias, J. C.; Marques, Susana; Branco, Pedro C.; Rodrigues, Thomas; Torres, Cristiana A.V.; Freitas, Filomena; Evtuguin, Dmitry; Silva, CarlaABSTRACT: In line with environmental awareness movements and social concerns, the textile industry is prioritizing sustainability in its strategic planning, product decisions, and brand initiatives. The use of non-biodegradable materials, obtained from non-renewable sources, contributes heavily to environmental pollution throughout the textile production chain. As sustainable alternatives, considerable efforts ...
Bioremediation of Synthetic Wastewater with Contaminants of Emerging Concern by...
Santos, Bruna; Araújo, Juliana; Carvalho, Beatriz; Cotrim, Carolina; Bernardino, Raul; Freitas, Filomena; Sobral, Abílio J. F. N.; Encarnação, TelmaContaminants of emerging concern (CECs) pose a potential risk to human and environmental health. Microalgae bioremediation is a promising approach for transforming or removing contaminants from the environment, while contributing to the circular economy. In this study, Nannochloropsis sp. was effectively used for the simultaneous removal of six CECs: paracetamol, ibuprofen, imidacloprid, methylparaben and bisph...
Bioprocess model-predictive control with physics-informed neural networks
Catalão, Marta; Pinto, José; Torres, Criatiana A. V.; Freitas, Filomena; Reis, Maria; Costa, Rafael S.; Oliveira, RuiMany previous studies have investigated the economic production of polyhydroxyalkanoates (PHA) by natural microbiomes. A key underlying strategy is the feast and famine (F/F) feeding regimen for bacteria selection. For this purpose, a sequencing batch reactor (SBR) is commonly operated in a sequence of F/F cycles until an evolved microbiome is attained with high PHA storage capacity. The effectiveness of this p...
Heterotrophic cultivation of Chlorella vulgaris yellow mutant on sidestreams: M...
Trovão, Mafalda; Barros, Ana; Machado, Adriana; Reis, Ana; Pedroso, Humberto; Espírito Santo, Gonçalo; Correia, Nádia; Costa, Monya; Ferreira, SaraMicroalgal protein is a promising feedstock to complement and/or replace other protein sources. Besides requiring less land and water usage, microalgae production is a more sustainable process, especially if industrial sidestreams are used as nutrient sources. Additionally, the heterotrophic cultivation of microalgae, such as Chlorella vulgaris, enables the achievement of much higher biomass productivity and lo...
Improving the heterotrophic media of three chlorella vulgaris mutants toward op...
Trovão dos Santos, Mafalda; Cunha, Miguel; Santo, Gonçalo Espírito; Pedroso, Humberto; Reis, Ana; Barros, Ana; Correia, Nádia; Schüler, LisaThe high production costs and unappealing sensory properties still limit the widespread commercialization of microalgae feedstocks. Therefore, this work focused on fine-tuning the heterotrophic medium composition to cultivate novel green, yellow, and white Chlorella vulgaris mutant strains. Screening assays were carried out to select the most significant factors, and different nutrient concentrations were optim...
A Validated RP-HPLC Method for Monitoring Pollutants Removal during Microalgae ...
Santos, Bruna; Araújo, Juliana; Freitas, Filomena; Sobral, Abílio J.F.N.; Encarnação, TelmaA Reversed-Phase High-Performance Liquid Chromatography (RP-HPLC) method was developed and validated to simultaneously quantify six contaminants of emerging concern (CECs) in the context of microalgae bioremediation. RP-HPLC was chosen for its ability to separate substances of differing polarities, its use of cost-effective and low-toxicity mobile phases, and the capacity to selectively adjust pH for enhanced s...
Polyhydroxyalkanoate Production from Eucalyptus Bark's Enzymatic Hydrolysate
Rodrigues, Thomas; Torres, Cristiana A. V.; Marques, Susana; Gírio, Francisco; Freitas, Filomena; Reis, Maria A.ABSTRACT: In recent years, polyhydroxyalkanoates (PHAs) have gained notoriety because of their desirable properties that include proven biodegradability, biocompatibility, and thermal stability, which make them suitable alternatives to fossil-based polymers. However, the widespread use of PHAs is still challenging because of their production costs, which are greatly associated with the cultivation medium used f...
Oxyfluorfen: a novel metabolic inhibitor to select microalgal chlorophyll-defic...
Trovão dos Santos, Mafalda; Cardoso, Lucas; Schüler, Lisa; Machado, Adriana; Santo, Gonçalo Espírito; Pedroso, Humberto; Reis, Ana; Barros, AnaNowadays, there is an increasing demand for novel feedstocks and alternative protein sources to meet global needs. Because of their rich nutritional profiles and high protein contents, microalgae-based food products and supplements are being developed. Nonetheless, these products present organoleptic characteristics such as taste, smell and colour that are often considered unpleasant by human and animal consume...