9 documents found, page 1 of 1
Neuroprotective effects on microglia and insights into the structure–activity r...
Plácido, Alexandra; Amaral, Constança Pais Do; Teixeira, Cátia; Nogueira, Ariane; Brango‐Vanegas, José; Alves Barbosa, Eder; C. Moreira, DanielTryptophyllins constitute a heterogeneous group of peptides that are one of the first classes of peptides identified from amphibian's skin secretions. Here, we report the structural characterization and antioxidant properties of a novel tryptophyllin-like peptide, named PpT-2, isolated from the Iberian green frog Pelophylax perezi. The skin secretion of P. perezi was obtained by electrical stimulation and fract...
Neuroprotective effects on microglia and insights into the structure–activity r...
Plácido, Alexandra; Amaral, Constança do Pais do; Teixeira, Cátia; Nogueira, Ariane; Brango-Vanegas, José; Barbosa, Eder Alves; Moreira, Daniel C.Tryptophyllins constitute a heterogeneous group of peptides that are one of the first classes of peptides identified from amphibian’s skin secretions. Here, we report the structural characterization and antioxidant properties of a novel tryptophyllin-like peptide, named PpT-2, isolated from the Iberian green frog Pelophylax perezi. The skin secretion of P. perezi was obtained by electrical stimulation and fract...
TNF-alpha-induced microglia activation requires miR-342: impact on NF-kB signal...
Brás, João Paulo; Bravo, Joana; Freitas, Jaime; Barbosa, Mário Adolfo; Santos, Susana Gomes; Summavielle, TeresaGrowing evidences suggest that sustained neuroinflammation, caused by microglia overactivation, is implicated in the development and aggravation of several neurological and psychiatric disorders. In some pathological conditions, microglia produce increased levels of cytotoxic and inflammatory mediators, such as tumor necrosis factor alpha (TNF-α), which can reactivate microglia in a positive feedback mechanism....
The antioxidant peptide salamandrin-i: First bioactive peptide identified from ...
Plácido, Alexandra; Bueno, João; Barbosa, Eder A.; Moreira, Daniel C.; Dias, Jhones Do Nascimento; Cabral, Wanessa Felix; Albuquerque, PatríciaMade available in DSpace on 2020-12-12T02:01:06Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-04-01; Universidade Estadual Paulista; Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES); Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq); Fundação para a Ciência e a Tecnologia; Amphibian skin is a multifunctional organ that plays key roles in defense, breathing, and wat...
Neutral PEGylated liposomal formulation for efficient folate-mediated delivery ...
Nogueira, E.; Freitas, Jaime; Loureiro, Ana Isabel Sá; Nogueira, Patrícia; Gomes, Andreia C; Preto, Ana; Carmo, Alexandre M.; Moreira, AlexandraCationic liposomes are efficient vectors for systemic delivery of therapeutic small interfering RNA (siRNA), taking advantage of RNA interference (RNAi), a naturally occurring gene-silencing mechanism in mammalian cells. However, toxicity at high concentrations, short circulating half-lives and lack of specificity restrict their successful application in a wider scale. The purpose of this study was to evaluate ...
Assessment of liposome disruption to quantify drug delivery in vitro
Nogueira, E.; Cruz, Célia F.; Loureiro, A.; Nogueira, Patrícia; Freitas, Jaime; Moreira, Alexandra; Carmo, Alexandre M.; Gomes, Andreia C; Preto, AnaEfficient liposome disruption inside the cells is a key for success with any type of drug delivery system. The efficacy of drug delivery is currently evaluated by direct visualization of labeled liposomes internalized by cells, not addressing objectively the release and distribution of the drug. Here, we propose a novel method to easily assess liposome disruption and drug release into the cytoplasm. We propose ...
Peptide anchor for folate-targeted liposomal delivery
Nogueira, E.; Mangialavori, Irene C.; Loureiro, Ana; Azóia, Nuno G.; Sarria, Marisa P.; Nogueira, Patrícia; Freitas, Jaime; Harmark, JohanSpecific folate receptors are abundantly overexpressed in chronically activated macrophages and in most cancer cells. Directed folate receptor targeting using liposomes is usually achieved using folate linked to a phospholipid or cholesterol anchor. This link is formed using a large spacer like polyethylene glycol. Here, we report an innovative strategy for targeted liposome delivery that uses a hydrophobic fra...
Enhancing methotrexate tolerance with folate tagged liposomes in arthritic mice
Nogueira, Eugénia Sofia Costa; Lager, Franck; Le Roux, Delphine; Nogueira, Patrícia; Freitas, Jaime; Charvet, Celine; Renault, Gilles; Loureiro, AnaMethotrexate is the first line of treatment of rheumatoid arthritis. Since many patients become unresponsive to methotrexate treatment, only very expensive biological therapies are effective and increased methotrexate tolerance strategies need to be identified. Here we propose the encapsulation of methotrexate in a new liposomal formulation using a hydrophobic fragment of surfactant protein conjugated to a link...
Liposome and protein based stealth nanoparticles
Nogueira, Eugénia Sofia Costa; Loureiro, Ana; Nogueira, Patrícia; Freitas, Jaime; Almeida, Catarina R.; Harmark, Johan; Hebert, Hans; Moreira, AlexandraLiposomes and protein based nanoparticles were tuned with different polymers and glycolipids to improve stealth and thus decrease their clearance by macrophages. Liposomes were coated with polyethylene glycol (PEG) and brain-tissue-derived monosialoganglioside (GM1). Bovine serum albumin (BSA) nanoparticles were produced incorporating a PEGylated surfactant (PEG-surfactant). All obtained nanoparticles were mono...