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Synthetic peptides as an antigenic base in an ELISA for laboratory diagnosts of...
Oliveira, Edward José de; Kanamura, HerminiaYohko; Kioko Takei; Hirata, Rosario Dominguez Crespo; Valli, Luiz Carlos Pedrosa; Nguyen, Nga YenMade available in DSpace on 2019-09-12T16:53:43Z (GMT). No. of bitstreams: 0 Previous issue date: 2008; A pool of five synthetic peptides was used as an antigenic base in an ELISA (ELISA-Pp) for laboratory diagnosis of Schistosoma mansoni. Serum samples were obtained from individuals with acute (n = 23) and chronic (n = 30) schistosomiasis, with other parasitoses (n = 39) or without parasitic infections (n = 10...
Characterization of a clone from an adult worm cDNA library selected with anti-...
Valli, Luiz Carlos Pedrosa; Kanamura, Herminia Yohko; Cotrim, Paulo Cesar; Oliveira, Guilherme Correa; Oliveira, Edward José DeMade available in DSpace on 2019-09-11T20:58:41Z (GMT). No. of bitstreams: 0 Previous issue date: 2007; Considerando a escassez de antígenos quimicamente definidos, realmente úteis e confiáveis para aplicação na soroepidemiologia da esquistossomose em larga escala, foi proposto, neste trabalho, um método alternativo para a seleção de clones de cDNA que expressam proteínas com putativo potencial diagnóstico na e...
Characterization of a clone from an adult worm cDNA library selected with anti-...
Valli, Luiz Carlos Pedrosa; Kanamura, Herminia Yohko; Cotrim, Paulo Cesar; Oliveira, Guilherme Correa; Oliveira, Edward José deConsidering the scarcity of defined antigens, actually useful and reliable for use in the field studies, we propose an alternative method for selection of cDNA clones with potential use in the diagnosis of schistosomiasis. Human antibodies specific to a protein fraction of 31/32 kDa (Sm31/32), dissociated from immune complexes, are used for screening of clones from an adult worm cDNA library. Partial sequencing...