29 documents found, page 1 of 3
Insights towards the impact of subinhibitory chlorhexidine on antimicrobial sus...
Pereira, P; Almeida Santos, C; Duarte, B; Antunes, Patrícia; Peixe, L; Freitas, R; Novais, CarlaEnterococcus faecium, a human and animal commensal broadly distributed in the environment, is currently one of the most challenging multidrug-resistant (MDR) healthcare-associated pathogens worldwide. It is often exposed to chlorhexidine (CHX), a broad-spectrum antiseptic, extensively used in healthcare, domestic, and food production settings, and a diffused polluter. However, the impact of gradients of CHX con...
"Mannose glycans as key players in trained immunity: A novel anti-tumoral catal...
Almeida, P; Alves, I; Fernandes, Â; Lima, C; Freitas, R; Braga, I; Correia, J; Jerónimo, C; Pinho, SSCell wall glycans isolated from microorganisms are long known to provoke strong immune responses piloted by innate immune cell populations, including monocytes, in the context of Trained Immunity (TI). However, the contribution of yeast-derived mannan in the reprogramming of monocytes remains ill-defined. Here, we demonstrated that TI is often accompanied by an altered gene expression profile of selected glycan...
Complete blood count parameters as biomarkers of retinopathy of prematurity: a ...
Fevereiro-Martins, M; Santos, AC; Marques-Neves, C; Guimarães, H; Bicho, M; Afonso, C; Ferreira, J; Espírito Santo, R; Teixeira, F; Rosa, RPurpose: To evaluate complete blood count (CBC) parameters in the first week of life as predictive biomarkers for the development of retinopathy of prematurity (ROP). Methods: Multicenter, prospective, observational study of a cohort of preterm infants born with gestational age (GA) < 32 weeks or birth weight < 1500 g in eight Portuguese neonatal intensive care units. All demographic, clinical, and laboratory d...
Predictive Factors of Relapse After Methotrexate Discontinuation in Juvenile Id...
Azevedo, S; Tavares-Costa, J; Melo, AT; Freitas, R; Cabral, M; Conde, M; Aguiar, F; Neto, A; Mourão, AF; Oliveira-Ramos, F; Santos, MJ; Peixoto, DObjective: To identify predictive factors of relapse after discontinuation of Methotrexate (MTX) in Juvenile Idiopathic Arthritis (JIA) patients with inactive disease. Methods: We conducted a prospective multicenter cohort study of patients diagnosed with JIA using real world data from the Portuguese national register database, Reuma.pt. Patients with JIA who have reached JADAS27 inactive disease and discontinu...
Glycoproteomics identifies HOMER3 as a potentially targetable biomarker trigger...
Peixoto, A; Ferreira, D; Azevedo, R; Freitas, R; Fernandes, E; Relvas-Santos, M; Gaiteiro, C; Soares, J; Cotton, S; Teixeira, B; Paulo, P; Lima, LBackground: Muscle invasive bladder cancer (MIBC) remains amongst the deadliest genitourinary malignancies due to treatment failure and extensive molecular heterogeneity, delaying effective targeted therapeutics. Hypoxia and nutrient deprivation, oversialylation and O-glycans shortening are salient features of aggressive tumours, creating cell surface glycoproteome fingerprints with theranostics potential. Meth...
Epigenetic regulation of cdh1 is altered after hoxb7-silencing in mda-mb-468 tr...
Paço, A; Leitão-Castro, J; Freitas, RHOXB7 is often overexpressed in breast cancer cells and found to relate to poor prognosis. The search for the HOXB7 targets, as a transcription factor, has led to molecules involved in regulating cell proliferation, migration, invasion, and processes such as angiogenesis and therapy resistance. However, the specific targets affected by the deregulation of HOXB7 in breast cancer remain largely unknown in most mo...
Hoxb7 overexpression leads triple-negative breast cancer cells to a less aggres...
Garcia, SAB; Araújo, M; Pereira, T; Freitas, RHOX genes appear to play a role in breast cancer progression in a molecular subtype-dependent way. The altered expression of HOXB7, for example, was reported to promote breast cancer progression in specific subtypes. Here we induced HOXB7 overexpression in MDA-MB-231 cells, a cellular model of the Triple-Negative breast cancer molecular subtype, and evaluated the phenotypic changes in cell viability, morphogene...
Roles of the hox proteins in cancer invasion and metastasis
Paço, A; Garcia, SAB; Leitão-Castro, J; Costa-Pinto, AR; Freitas, RInvasion and metastasis correspond to the foremost cause of cancer-related death, and the molecular networks behind these two processes are extremely complex and dependent on the intra-and extracellular conditions along with the prime of the premetastatic niche. Currently, several studies suggest an association between the levels of HOX genes expression and cancer cell invasion and metastasis, which favour the ...
Hoxd13/Bmp2-mediated mechanism involved in zebrafish finfold design
Castro, J; Beviano, V; Paço, A; Leitão-Castro, J; Cadete, F; Francisco, M; Freitas, RThe overexpression of hoxd13a during zebrafish fin development causes distal endochondral expansion and simultaneous reduction of the finfold, mimicking the major events thought to have happened during the fin-to-limb transition in Vertebrates. We investigated the effect of hoxd13a overexpression on putative downstream targets and found it to cause downregulation of proximal fin identity markers (meis1 and emx2...
Knowledge and Use of PEP and PrEP Among Key Populations Tested in Community Cen...
Simões, D; Meireles, P; Rocha, M; Freitas, R; Aguiar, A; Barros, HBackground: Pre-exposure prophylaxis (PrEP) and post-exposure prophylaxis (PEP) have been increasingly available in Europe. Due to the high burden of HIV in key populations, these could benefit from their use. In 2016, in Portugal, an open, non-interval, prospective cohort study was established in a network of 26 community-based voluntary HIV/STI counseling and testing centers. Data collected included questions...