8 documents found, page 1 of 1
NAD(P)H–FAD fluorescence detection using multiphoton microscopy improved with h...
Freitas, Ruben Brandão; Rodrigues, Manuel José Lima Ferreira; Cunha, Nelssom F.; Pimenta, Sara; Belsley, Michael; Correia, J. H.; Maciel, Marino J.Early detection of cancer is critical for improving patient survival rates, reducing morbidity and minimizing treatment costs. This study explores the use of multiphoton microscopy to enhance colorectal cancer detection, by using two-photon excitation fuorescence of intrinsic fuorophores. The diagnostic capability of this technique hinges on two key metabolic enzymes, nicotinamide adenine dinucleotide and favin...
The chick chorioallantoic membrane assay as an in vivo model for colon tumor an...
Pereira, Hugo Martins; Freitas, Ruben Brandão; Maciel, Marino Jesus Correia; Pimenta, Sara Filomena Ribeiro; Costa, Marta Sílvia FreitasOptical coherence tomography (OCT) has emerged as a promising imaging modality for biomedical research. In this paper, a commercial OCT system was used to differentiate two colon cell lines implanted in a chick embryo chorioallantoic membrane (CAM) model, using the attenuation coefficient of light. Malignant and non-malignant tumors were developed from RKO and NCM460 cell lines, respectively. OCT B-scan images ...
A polymorph of dipeptide halide glycyl-L-alanine hydroiodide monohydrate: cryst...
Baptista, Rosa Maria Ferreira; Gomes, Clara S. B.; Silva, Bruna Machado; Oliveira, joão; Almeida, B. G.; Castro, Maria Cidália RodriguesA polymorph of glycyl-L-alanine HI.H2O is synthesized from chiral cyclo-glycyl-L-alanine dipeptide. The dipeptide is known to show molecular flexibility in different environments, which leads to polymorphism. The crystal structure of the glycyl-L-alanine HI.H2O polymorph is determined at room temperature and indicates that the space group is polar (P21), with two molecules per unit cell and unit cell parameters...
Advancing cancer detection: Multi-photon microscopy setup for NADH and FAD fluo...
Freitas, Ruben Brandão; Rodrigues, Manuel José Lima Ferreira; Pimenta, Sara Filomena Ribeiro; Belsley, M.; Correia, J. H.; Maciel, Marino Jesus CorreiaMulti-photon microscopy (MPM) revolutionizes cancer diagnosis and can replace histopathology during colonoscopy. Based on endogenous fluorophores such as NADH and FAD, MPM offers high sensitivity. This technique enables real-time imaging and optical sectioning, potentially enhancing early-stage colon cancer detection [1,2]. The focus of this work is on the implementation of an MPM setup for NADH and FAD detecti...
Multi-photon microscopy setup for integration in colonoscopes: an overview
Freitas, Ruben Brandão; Maciel, Marino Jesus Correia; Pimenta, Sara Filomena Ribeiro; Belsley, M.; Correia, J. H.[Excerpt] Introduction Multi-photon microscopy (MPM) is considered one of the breakthrough inventions. This technique is based on the intracellular endogenous fluorescent signal, such as the two-photon excited fluorescence and second-harmonic generation (SHG) [1–3]. MPM provides optical sectioning from deep specimens with high scattering properties, restricting fluorophore excitation to the focal plane [4]. Thi...
Flexible and reduced-size neural probe using a photosensitive polyimide
Freitas, João Rui Martins; Freitas, Ruben Brandão; Esteves, Bruno Miguel Carpinteiro; Santos, Diogo Juvandes Freitas; Maciel, Marino Jesus CorreiaThe understanding of the human brain function is one of the main challenges of modern science, as it comprises the study of an extremely complex organ. The majority of neural interfaces to study the brain rely on non-flexible and relatively thick structures, which do not adapt to the plastic nature of the brain, damaging the tissue and compromising data acquisition [1, 2]. This research presents the fabrication...
Detection of defects on displays based on microscopic and optical coherence tom...
Pereira, Hugo Martins; Freitas, Ruben Brandão; Esteves, Bruno Miguel Carpinteiro; Santos, C. P.; Carvalho, Pedro; Compadrinho, Paulo; Rodrigues, Carlos[Excerpt] Introduction The use of displays in control car panels, smartphones, notebooks and LCD TVs has increased in the last decade. The large-scale manufacturing of new products emphasizes the need for fault detection at an early stage in the assembly process to detect the presence of scratches and particles. Therefore, fast, reliable and efficient displays inspection equipment is essential in order to reduc...
Cápsula insuflável para o tratamento da obesidade
Freitas, Ruben BrandãoO tratamento da obesidade quando baseado apenas em alterações do estilo de vida revela-se ineficaz a longo prazo. Existem tratamentos mais definitivos, contudo são normalmente muito invasivos, cirurgicamente complexos e acarretam um elevado risco para o paciente. O tratamento com balão insuflável é uma alternativa definitiva e com uma complexidade cirúrgica reduzida comparativamente aos restantes métodos. No en...