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Analysis of protein expression and a new prokaryotic expression system for goat...

Rádis-Baptista, Gandhi; Cajazeiras, João Batista; Melo, Luciana Magalhães; Albuquerque, Érica Larissa M. Souto de; Cavada, Benildo Sousa

Low purification efficiency and incomplete characterization of male goat (buck) spermadhesins (Bdhs) prompted us to develop an effective system to produce recombinant Bdhs (rBdhs). Bdh-2 cDNA was inserted into a prokaryotic expression plasmid, pTrcHis TOPO. The pTrcHis-Bdh-2 system was constructed to produce a His6 fusion protein in Escherichia coli Top10 cells. Recombinant clones were selected by growth in amp...

Date: 2021   |   Origin: Oasisbr
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