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Comparative analysis of polysaccharide and nutritional composition of biologica...

Araújo-Rodrigues, Helena; Amorim, Manuela; Freitas, Victor de; Relvas, João B.; Tavaria, Freni K.; Pintado, Manuela

This study chemically characterized three Pleurotus ostreatus fruiting bodies cultivated in the Iberian Peninsula under different conditions (biological and industrial), with emphasis on polysaccharide analysis. Comprehensive comparative data on cultivation-dependent nutritional variations will potentially improve their nutritional and therapeutic applications. Industrial mushrooms (POC and POA) contained signi...


Acidochromic free-standing multilayered chitosan-pyranoflavylium/alginate membr...

Cunha, Mariana; Freitas, Victor de; Borges, João; Mano, João F.; Rodrigues, João M. M.; Cruz, Luís

Food smart packaging has emerged as a promising technology to address consumer concerns regarding food conservation and food safety. In this context, we report the rational design of azide-containing pyranoflavylium-based pH-sensitive dye for subsequent click chemistry conjugation toward a chitosan-modified alkyne. The chitosan-pyranoflavylium conjugate was characterized by infrared (ATR-FTIR), ultraviolet−visi...


Surfactin and polyglutamic acid coproduction by Bacillus velezensis P#02 using ...

Correia, Jéssica Alexandra Henriques; Gudiña, Eduardo José; Janek, Tomasz; Dias, Ricardo; Teixeira, J. A.; Freitas, Victor de

Bacillus velezensis P#02 simultaneously produced surfactin and polyglutamic acid (PGA). Among the different culture media studied, the one containing corn steep liquor (100 mL/L), glucose (10 g/L), and glutamic acid (10 g/L) as sole ingredients (CSLGGlut(10)) offered the best results regarding biosurfactant and biopolymer production. Although biosurfactant production occurred both under shaking and static condi...


First insight into lignin valorization as a promising biopolymer for the modula...

Pereira, Ana Rita; Brandão, Elsa; Pinto, Joana; Pinho, Paula Guedes de; Costa, Carina A. E.; Rodrigues, Alírio E.; Michelin, Michele; Soares, Susana

Lignosulfonate (LS), kraft lignin (KL), and organosolv lignin (OL) were evaluated as potential modulating agents of the physicochemical properties of Port wine at two different concentrations for 7 and 30 days. KL and LS demonstrated the ability to remove proteins and potentiate the anthocyanin concentration. LS reduced the tannin content and the interaction of salivary acidic proline-rich proteins with wine ph...


Type 2 Diabetes mellitus alters the cargo of (poly)phenol metabolome and the ox...

Reis, Ana; Rocha, Sara; Dias, Irundika HK.; Costa, Raquel; Soares, Raquel; Sánchez-Quesada, José Luis; Perez, Antonio; Freitas, Victor de

The incidence of diabetes on the worldwide population has tripled in the past 5 decades. While drug-based therapies are valuable strategies to treat and ease the socio-economic burden of diabetes, nutritional strategies offer valuable alternatives to prevent and manage diabetes onset and contribute to the sustainability of health budgets. Whilst, intervention studies have shown that (poly)phenol-rich diets impr...


Upcycling rocha do Oeste pear pomace as a sustainable food ingredient: composit...

Fernandes, Ana; Simões, Sara; Ferreira, Isabel M. P. L. V. O.; Alegria, Maria João; Mateus, Nuno; Raymundo, Anabela; Freitas, Victor de

This work explores the potential of Rocha do Oeste pear pomace to be used as a sustainable and healthy food ingredient. Moreover, the enrichment with yeast protein extract (YPE) may be useful to design innovative food products. The main goals of this study were to assess pear pomace concerning: (i) chemical composition and antioxidant capacity; (ii) rheology, texture, and microstruc- ture characterization (alon...


Wine astringent compounds monitored by an electrochemical biosensor

Costa, Joana J.; Moreira, Felismina; Soares, Susana; Brandão, Elsa; Mateus, Nuno; Freitas, Victor de; Sales, Goreti

An innovative approach for monitoring astringent polyphenols in beverages (wines) is described, consisting of an electrochemical biosensor constructed by adsorbing salivary α-amylase or proline-rich protein (PRP) onto amined gold screen-printed electrodes. Interaction with polyphenols was tested using pentagalloyl glucose (PGG) as a standard, an important representative element for astringency. The analytical p...


Sustainable chitosan packaging films: Green tea polyphenolic extraction strateg...

Alves, Tiago Filipe P.; Teixeira, Natércia; Vieira, J.; Vicente, A. A.; Mateus, Nuno; Freitas, Victor de; Souza, Hiléia K. S.

In this work, an alternative approach towards the use of a ternary natural deep eutectic solvent (ChCl/glycerol/lactic acid: NADES-3) as an environmentally friendly medium for the extraction of polyphenolic compounds from green tea (GT) was investigated. The ternary NADES have a higher capacity of polyphenolic extraction in comparison with the other two binary NADES (NADES-1 and NADES-2, ChCl/glycerol and ChCl/...


Identification of gallotannins and ellagitannins in aged wine spirits: a new pe...

Fernandes, Tiago; Antunes, Alexandra M. M.; Caldeira, Ilda; Anjos, Ofélia; Freitas, Victor de; Fargeton, Laurent; Boissier, Benjamin; Catarino, Sofia

This research was focused on identifying gallotannins and ellagitannins degradation pathways to better understand their behavior in complex media such as wine spirits (WS). A WS was aged with chestnut wood staves with three levels of micro-oxygenation, nitrogen, and using wooden barrels. Gallotannins and ellagitannins were identified by LC-ESI-HRMS/MS using a Q-TOF in samples collected at 8, 21, 60, 180, 270, a...


Polyphenol extraction by deep eutectic solvent for valorisation of portuguese g...

Alves, Tiago Filipe P.; Teixeira, Natércia; Vieira, Jorge; Vicente, A. A.; Freitas, Victor de; Souza, Hiléia K. S.

The current demand for antioxidant active packaging, that is achieved by introducing antioxidants into food packaging material, is increasing due to both the unquestionable advantages compared to the addition of antioxidants directly to the food and the tendency to consume healthy and fresh products. When developing active packaging materials, research has been focused on natural and biodegradable polymers (suc...


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