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Production, purification and characterization of laccase from pleurottus ostrea...

Freixo, Maria do Rosário; Karmali, Amin; Arteiro, José Maria Santos

A strain of Pleurotus ostreatus was grown in tomato pomace as sole carbon source for production of laccase. The culture of P. ostreatus revealed a peak of laccase activity (147 U/L of fermentation broth) on the 4th day of culture with a specific activity of 2.8 U/mg protein. Differential chromatographic behaviour of laccase was investigated on affinity chromatographic matrices containing either urea, acetamide,...


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