8 documents found, page 1 of 1
Gliding motility protein LIMP promotes optimal mosquito midgut traversal and in...
Egarter, Saskia; Santos, Jorge M.; Kehrer, Jessica; Sattler, Julia; Frischknecht, Friedrich; Mair, GunnarSubstrate-dependent gliding motility is key to malaria transmission. It mediates host cell traversal, invasion and infection by Plasmodium and related apicomplexan parasites. The 110 amino acid-long cell surface protein LIMP is essential for P. berghei sporozoites where it is required for the invasion of the mosquito’s salivary glands and the liver cells of the rodent host. Here we define an additional role for...
Intravital imaging of host–parasite interactions in skin and adipose tissues
De Niz, Mariana; Meehan, Gavin R.; Brancucci, Nicolas M. B.; Marti, Matthias; Rotureau, Brice; Figueiredo, Luisa M.; Frischknecht, FriedrichIntravital microscopy allows the visualisation of how pathogens interact with host cells and tissues in living animals in real time. This method has enabled key advances in our understanding of host-parasite interactions under physiological conditions. A combination of genetics, microscopy techniques, and image analysis have recently facilitated the understanding of biological phenomena in living animals at cel...
Toolbox for In Vivo imaging of host–parasite interactions at multiple scales
De Niz, Mariana; Spadin, Florentin; Marti, Matthias; Stein, Jens V.; Frenz, Martin; Frischknecht, FriedrichAnimal models have for long been pivotal for parasitology research. Over the last few years, techniques such as intravital, optoacoustic and magnetic resonance imaging, optical projection tomography, and selective plane illumination microscopy developed promising potential for gaining insights into host-pathogen interactions by allowing different visualization forms in vivo and ex vivo. Advances including incre...
Functional genetic evaluation of DNA house-cleaning enzymes in the malaria para...
Kumar, Hirdesh; Kehrer, Jessica; Singer, Mirko; Reinig, Miriam; Santos, Jorge M.; Mair, Gunnar R.; Frischknecht, FriedrichBackground: Cellular metabolism generates reactive oxygen species. The oxidation and deamination of the deoxynucleoside triphosphate (dNTP) pool results in the formation of non-canonical, toxic dNTPs that can cause mutations, genome instability, and cell death. House-cleaning or sanitation enzymes that break down and detoxify non-canonical nucleotides play major protective roles in nucleotide metabolism and con...
The Plasmodium palmitoyl-S-acyl-transferase DHHC2 is essential for ookinete mor...
Santos, Jorge M.; Kehrer, Jessica; Franke-Fayard, Blandine; Frischknecht, Friedrich; Janse, Chris J.; Mair, GunnarThe post-translational addition of C-16 long chain fatty acids to protein cysteine residues is catalysed by palmitoyl-S-acyl-transferases (PAT) and affects the affinity of a modified protein for membranes and therefore its subcellular localisation. In apicomplexan parasites this reversible protein modification regulates numerous biological processes and specifically affects cell motility, and invasion of host c...
Highly dynamic host actin reorganization around developing Plasmodium inside he...
Gomes-Santos, Carina S. S.; Itoe, Maurice A.; Afonso, Cristina; Henriques, Ricardo; Gardner, Rui; Sepúlveda, Nuno; Simões, Pedro D.; Raquel, HelenaPlasmodium sporozoites are transmitted by Anopheles mosquitoes and infect hepatocytes, where a single sporozoite replicates into thousands of merozoites inside a parasitophorous vacuole. The nature of the Plasmodium-host cell interface, as well as the interactions occurring between these two organisms, remains largely unknown. Here we show that highly dynamic hepatocyte actin reorganization events occur around ...
Highly dynamic host actin reorganization around developing Plasmodium inside he...
Gomes-Santos, Carina S S; Itoe, Maurice A; Afonso, Cristina; Henriques, Ricardo; Gardner, Rui; Sepúlveda, Nuno; Simões, Pedro D; Raquel, HelenaPlasmodium sporozoites are transmitted by Anopheles mosquitoes and infect hepatocytes, where a single sporozoite replicates into thousands of merozoites inside a parasitophorous vacuole. The nature of the Plasmodium-host cell interface, as well as the interactions occurring between these two organisms, remains largely unknown. Here we show that highly dynamic hepatocyte actin reorganization events occur around ...
Highly dynamic host actin reorganization around developing Plasmodium inside he...
Gomes Santos, Carina S. S.; Itoe, Maurice; Afonso, Cristina; Henriques, Ricardo; Gardner, Rui; Sepulveda, Nuno; Simões, Pedro D.; Raquel, Maria HelenaPlasmodium sporozoites are transmitted by Anopheles mosquitoes and infect hepatocytes, where a single sporozoite replicates into thousands of merozoites inside a parasitophorous vacuole. The nature of the Plasmodium-host cell interface, as well as the interactions occurring between these two organisms, remains largely unknown. Here we show that highly dynamic hepatocyte actin reorganization events occur around ...